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Correlation of Individual Heterozygosity of microsatellite marker Loci with Heterosis of Growth Traits in Pig Populations

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Agricultural Sciences in China 2006年第8期5卷 635-642页

作者： ZHANG Jing-hu XIONG Yuan-zhu DENG Chang-yan JIANG Si-wen LEI Ming-gang LI Jia-lian LI Feng-e ZHENG Rong Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/College of Animal Science and Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R.China Department of Biology Science ＆ Technology Zhangzhou Teachers College Zhangzhou 363000 P.R.China

To investigate the correlation of individual heterozygosity and heterosis of three traits in crossbred F1 pig populations, the F1 populations were built by random mating Yorkshire x Meishan （YM, n = 82）, and its reciprocal （MY, n =47） and two straightbred populations （Yorkshire = 34, Meishan = 55） were used as control groups. The heterosis of birth weight （BWT）, average daily gain （ADG）, and feed conversion ratio （FCR） were acquired as well. In the research, the significant marker loci for the heterosis of the three traits were observed by one-way ANOVA （P〈0.01） in a total of 39 marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13, and the numbers of the significant marker loci were 12 （BWT）, 18 （ADG）, and 17 （FCR）, respectively, based on which the general heterozygosity （GH） was divided into significant marker loci heterozygosity （SH） and insignificant marker loci heterozygosity （IH）. Furthermore, the trends of alteration in heterosis with the stepwise increase in heterozygosity by 0.05 were explored. This was done by the regression analysis of the three kinds of heterozygosity against heterosis of the three traits. The results showed that, for BWT, the heterosis increased with the increase in GH （r=0.9337, P=0.0021） and SH （r=0.9165, P=0.0102）; for ADG, the heterosis increased with the increase in IH （r=0.7012, P=0.0353） and GH （r=0.7470, P=0.0537, near significant）; for FCR, the heterosis of feed efficiency increased with the increase in IH （r=0.8721, P=0.0022）. The results indicated that the correlation was not always higher or more significant for SH with heterosis than it was for IH or GH with heterosis, and it might be because of the reciprocal cancellation of the positive effect and negative effect of QTL linked to the significant marker loci.

关键词： pig heterosis microsatellite marker individual heterozygosity

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Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella)

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Journal of Genetics and Genomics 2008年第4期35卷 201-206页

作者： Cheng Yang Xinping Zhu Xiaowen Sun College of Aqua-Life Science and Technology Shanghai Fisheries University Shanghai 200090 China Heilongjiang River Fisheries Research Institute Chinese Academy of Fishery Sciences Harbin 150076 China Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou 510380 China

microsatellite markers have been increasingly used in genetic studies on fishery species because of their high applicability in selective breeding programs. Here we reported the development of microsatellite markers and their utilization in mud carp （Cirrhina molitorella）. An （CA）15 enriched library has been constructed for mud carp, using the magnetic beads enrichment procedure. Sequence analysis of 60 randomly picked positive colonies indicate that 56 （93.3%） of the colonies contain microsatellites. microsatellite polymorphism was assessed using 10 mud carp individuals, and 12 microsatellite loci turned out to be polymorphic. We utilized these loci to study the genetic diversity of a wild population （WM） and a cultured population （CM） of the mud carp. A total of 109 alleles were detected with an average of 9.08 alleles per locus. The mean value of the observed heterozygosity of WM and CM was 0.6361 and 0.6417, respectively, and significant decrease of genetic diversity in CM was not observed. The genetic distance between the two populations was 0.1546 and the value of Gsr was 0.0473. This showed that there existed a slight genetic differentiation between WM and CM.

关键词： microsatellite marker genetic diversity mud carp Cirrhina molitorella

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Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers

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Acta Oceanologica Sinica 2011年第3期30卷 76-83页

作者： LIU Yunguo GUO Yinghui LIU Shuaishuai LI Junfeng YE Naihao Inspection ＆ Quarantine Technology Center Shandong Entry-Exit Inspection ＆ Quarantine Bureau Qingdao 266002 China Ocean University of China Qingdao 266003 China College of Basic Medical Sciences Shandong University of Traditional Chinese Medicine Jinan 250355 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China College of Chemical Engineering Qingdao University of Science ＆ Technology Qingdao 266042 China

Ten highly variable microsatellite loci were performed in order to evaluate the genetic variability of wild and hatchery samples of half-smooth tongue sole Cynoglossu＇s semilaevis. A group of 200 genotypes belonging to four wild samples, Laizhou （LZ）, Weihai （WH）, Qingdao （QD）, Rizhao （RZ） and one hatchery sample, Mingbo （MB）, were screened. All of the： ten microsatellite loci screened in this study showed marked polymorphism. A total of 70 different alleles were observed over all loci. The number of alleles per locus ranged from 4.2 to 1006 the average of expected and observed heterozygosity ranged from 0.67 to 0.82, and from 0：77 to0.87 respectively. A total of 10, 16, 10, 11 and 5 unique alleles each were found in LZ, WH~ QD：RZ and MB populations. The effective number of alleles varied from 2.87 for HSTS_7 to 6.83 for HSTS_h. The number of average genotypes ranged from 6.0 for HSTS a to 15.4 for HSTS_h： As compared with the wild populations, the hatchery population, MB, showed significant genetic changes such as fewer alleles per locus （P〈0.05）, a smaller number of low frequency alleles （P〈0.05）, a small number of unique alleles and a small number of genotypes （P〈0.05）, all indicative of a reduction in genetic diversity.

关键词： Cynoglossus semilaevis microsatellite marker population genetic variability

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Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers

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Acta Oceanologica Sinica 2008年第4期27卷 156-164页

作者： CHANG Yumei DING Lei LI Mingyun XUE Liangyi LIANG Liqun HE Jianguo LEI Qingquan Heilongjiang River Fisheries Research Institute Chinese Academy of Fishery Sciences Harbin 150070China College of Life Sciences Zhongshan University Guangzhou 510275 China Life Science of Technology Institute Dalian Fisheries University Dalian 116023 China Life Science and Biotechnology Faculty Ningbo University Ningbo 315211 China College of Electrical ＆ Electronic Engineering Harbin University of Science and Technology Harbin 150080 China

The germplasm of breeding large yellow croaker （Pseudosciaena crocea Richardson） was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min - Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pair- wise differentiation coefficient（ FST ） was only 13. 1% between Daiqu and Min -Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min - Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences （P 〉 0. 05）.

关键词： Pseudosciaena crocea microsatellite marker bred stock germplasm admix

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Evaluation of genetic diversity and relationships within and between two breeds of duck based on microsatellite markers

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Progress in Natural Science:Materials International 2009年第11期19卷 1581-1586页

作者： Fei Wu~a,Yinghua Huang~a,Ying Ma~b,Shengqiang Hu~c,Jinping Hao~c,Ning Li~a a State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100193,China b College of Animal Science and Veterinary Science,Inner Mongolia Agricultural University,Hohhot 010018,China c Gold Star Duck Production Ltd.,Beijing 100076,China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China College of Animal Science and Veterinary Science Inner Mongolia Agricultural University Hohhot 010018 China Gold Star Duck Production Ltd. Beijing 100076 China

The genetic diversity of two natural populations(M,N) of Beijing duck(Anas platyrhynchos) and 11 artificially selected lines of Beijing duck(A,B,E-L,O) from China Gold Star Duck Production Ltd.,along with two Cherry Valley duck lines(C and D) from the British Cherry Valley Livestock Division,was evaluated using 18 microsatellite markers covering 16 linkage groups.A phylogenetic tree of the 15 populations of duck,formed of four main branches,was constructed from Nei’s DAgenetic distance.The mean genetic differentiation index(FST) in all loci,Nei’s standard genetic distance(Ds),and the genetic distance DAbetween the Beijing duck and the Cherry Valley duck were 0.075,0.143 and 0.142,respectively.These results demonstrated a high degree of genetic similarity between the two breeds and supported the hypothesis that the Cherry Valley duck was derived from the Beijing duck.The FSTmatrix of seven clusters of Beijing duck suggested that the efficiency of selection was not significant to some extent and should be supplemented by marker -assisted selection.

关键词： Duck microsatellite marker Genetic diversity Genetic distance

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Identification of quantitative trait loci for the dead leaf rate and the seedling dead rate under alkaline stress in rice

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Journal of Genetics and Genomics 2008年第5期35卷 299-305页

作者： Dongling Qi Guizhen Guo Myung-chul Lee Junguo Zhang Guilan Cao Sanyuan Zhang Seok-cheol Suh Qingyang Zhou Longzhi Han Institute of Crop Science Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement NFCR1/Key Laboratory of Crop Germplasm Resources and Biotechnology Ministry of Agriculture Beijing 100081 China Rubber Research Institute of China Chinese Academy of Tropical Agricultural Sciences/Key laboratory of Physiology for Tropical Crops Ministry of Agriculture Hainan 571737 China Institute of Rice Research Jilin Academy of Agricultural Sciences Gongzhuling 136100 China National Institute of Agricultural Biotechnology Rural Development Administration Suwon 441-707 Korea Horticultural Department Sichuan Agricultural University Ya＇an 625014 China

The quantitative trait loci （QTLs） for the dead leaf rate （DLR） and the dead seedling rate （DSR） at the different rice growing periods after transplanting under alkaline stress were identified using an F2：3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The DLR detected at 20 days to 62 days after transplanting under alkaline stress showed continuous normal or near normal distributions in F3 lines, which was the quantitative trait controlled by multiple genes. The DSR showed a continuous distribution with 3 or 4 peaks and was the quantitative trait controlled by main and multiple genes when rice was grown for 62 days after transplanting under alkaline stress. Thirteen QTLs associated with DLR were detected at 20 days to 62 days after transplanting under alkaline stress. Among these, qDLR9-2 located in RM5786-RM160 on chromosome 9 was detected at 34 days, 41 days, 48 days, 55 days, and 62 days, respectively; qDLR4 located in RM3524-RM3866 on chromosome 4 was detected at 34 days, 41 days, and 48 days, respectively; qDLR7-1 located in RM3859-RM320 on chromosome 7 was detected at 20 days and 27 days; and qDLR6-2 in RM1340-RM5957 on chromosome 6 was detected at 55 days and 62 days, respectively. The alleles of both qDLR9-2 and qDLR4 were derived from alkaline sensitive parent ＂Gaochanl06＂. The alleles of both qDLR7-1 and qDLR6-2 were from alkaline tolerant parent Changbai 9. These gene actions showed dominance and over dominance primarily. Six QTLs associated with DSR were detected at 62 days after transplanting under alkaline stress. Among these, qDSR6-2 and qDSR8 were located in RM1340-RM5957 on chromosome 6 and in RM3752-RM404 on chromosome 8, respectively, which were associated with DSR and accounted for 20.32% and 18.86% of the observed phenotypic variation, respectively; qDSR11-2 and qDSR11-3 were located in RM536-RM479 and RM2596-RM286 on chromosome 11, respectively, which were associated with

关键词： rice alkaline stress dead leaf rate dead seedling rate microsatellite marker quantitative trait locus （QTL）

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Development and Characterization of microsatellite markers in Brassica rapa ssp.chinensis and Transferability Among Related Species

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Agricultural Sciences in China 2008年第1期7卷 19-31页

作者： CUI Xiu-min DONG Yu-xiu HOU Xi-lin CHENG-Yan ZHANG Jing-yi JIN Min-feng National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R.China College of Resource and Environment Science Shandong Agricultural University Tai＇an 271018 P.R. China College of Life Science Shandong Agricultural University Tai＇an 271018 P.R.China

Simple sequence repeat （SSR） or microsatellite marker is a valuable tool for several purposes, such as mapping, fingerprinting, and breeding. In the present study, an intersimple sequence repeat （ISSR）-PCR technique was applied for developing SSR markers in non-heading Chinese cabbage （Brassica rapa）. A total of 190 SSRs were obtained. Among these, AG or CT （54.7%） was the most frequent repeat, followed by AC or GT （31.6%） of the microsatellites. The average number of the SSRs length array was 16 and 10 times, respectively. Based on the determined SSR sequences, 143 SSR primer pairs were designed to evaluate their transferabilities among the related species of Brassica. The number of alleles produced per marker averaged 2.91, and the polymorphism information content （PIC） value ranged from 0 to 0.863 with an average of 0.540. Monomorphism was observed in 16 primer pairs. The transferability percentage in CC genome was higher than in BB genome. More loci occurred in the BBCC genome. This result supported the hypothesis that BB genome was divergent from A and C genomes, and AA and CC genomes were relatively close. The polymorphic primers can be exploited for further evolution, fingerprinting, and variety identification.

关键词： Brassica rapa inter-simple sequence repeats （ISSR） microsatellite marker polymorphism transferability

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Analysis of expressed sequence tags （ESTs） from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis

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Insect Science 2015年第6期22卷 761-767页

作者： Hui Li Kun-Ling Lang Hai-Bin Fu Chang-Peng Shen Fang-Hao Wan Dong Chu Key Lab of Integrated Crop Pest Management of Shandong Province College of Agronomy and Plant Protection Qingdao AgriculturalUniversity Qingdao Shandong Province China Shenyang Entry-Exit Inspection and Quarantine Bureau Shenyang China StateKey Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences(CAAS) Beijing China

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags （ESTs） were analyzed from a normalized complementary DNA library of/？. solenopsis. In addition, EST-derived microsatellite loci （also known as simple sequence repeats or SSRs） were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.

关键词： expressed sequence tags microsatellite marker normalized cDNA library Phenacoccus solenopsis

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Kinship analysis reveals reproductive success skewed toward overwintered Brandt’s voles in semi-natural enclosures

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Integrative Zoology 2019年第5期14卷 435-445页

作者： Yan CHEN Dawei WANG Ning LI Xiangfa HU Fei REN Weili HAO Ying SONG Xiaohui LIU State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina College of Life Sciences Sichuan UniversityChengduChina

Age structure and seasonality influence the population fluctuations of small rodents.Age determines body weight and social experience,while seasonality regulates the duration of the breeding season and onset of sexual maturity in newborn offspring.Therefore,reproductive success and skew usually occur in different age groups.Brandt’s vole(Lasiopodomys brandtii)is a social,short-lived and seasonal breeding small rodent with a dramatic seasonal population fluctuation,but reproductive skew is not fully understood in this species.In the present study,we determined kinship in semi-natural enclosure populations using microsatellite markers based on genotyping,analyzed the reproductive skew between sexes and between overwintered and newborn voles,and monitored variation in male reproductive activity by testing fecal testosterone levels throughout the year.Overwintered voles had the most reproductive success along with a striking increase in the population size in the enclosures,with all biological fathers and 77.8%of biological mothers,which had 100%and 87%of the total offspring,respectively.Compared to overwintered voles,reproductive skews were significantly higher in potential overwintered and newborn parents,implying the possible reproductive suppression of newborn voles by dominant overwintered voles.Moreover,both heavier body weight and higher testosterone levels in overwintered males supported their potential social status in the population.Our study provided new evidence for reproductive skew and differentiation of postnatal gonadal development patterns of different age groups in Brandt’s vole.

关键词： Brandt’s vole microsatellite marker reproductive skew social dominance testosterone

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Performance and genetic diversity of 23 provenances of northern red oak(Quercus rubra L.)after 25 years of growth in South Korea

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Journal of Forestry Research 2021年第5期32卷 2181-2188页

作者： Ki-Won Kim Kyoung-Mi Lee Soon-Ho Kwon Ji-Min Park Yang-Gil Kim Hye-In Kang Da-Bin Yum Kyu-Suk Kang Department of Agriculture Forestry and BioresourcesSeoul National UniversitySeoul 08826Republic of Korea Department of Forest Bioresources National Institute of Forest ScienceSuwon 16631Republic of Korea Department of Plant Resource Research Korea National ArboretumPocheon 11186Republic of Korea Research Institute of Agriculture and Life Sciences CALS_SNUSeoul 08826Republic of Korea

Growth traits and genetic diversity of 23 provenances of Quercus rubra introduced from North America were analyzed in a provenance trial established with a randomized block design in Hwaseong,Gyeonggi,South Korea in 1993.Growth variables and survival at age 25 were compared with results from early stages.Height,DBH,volume and stem straightness of Q.rubra was better than those of the domestic oak(Quercus accutissima).Growth of the Dunham Island provenance from New York was the best among the 23 provenances that of the Eagle River provenance from Wisconsin was poorest.Survival rate at age25 was on average 52%.The longitude of seed origin and growth of provenance were consistently significantly negatively correlated at all ages.Growth of coastal provenances was superior to that of the inland provenances,which were separated by the Appalachian Mountains.Genetic diversity and genetic distance among the provenances were evaluated using micro satellite markers.The allelic frequencies showed high polymorphism in 10 microsatellite loci,and 292 alleles were found.Of 10 loci,two were commonly found in the 23 provenances.The mean allelic diversity and heterozygosity observed among the provenances were similar to those from the native populations of Q.rubra in North America.Nei’s genetic distance among the 23 was estimated and showed that a clear trend between geographic and genetic distances,indicating that some provenances have high genetic diversity with superior growth performance.

关键词： Introduction breeding Provenance test Adaptation microsatellite marker Genetic distance

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