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Regulation of actin cytoskeleton via photolithographic micropatterning

Journal of Innovative Optical Health Sciences 2023年第2期16卷 50-57页

作者： Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu Imshik Lee Leiting Pan Jingjun Xu The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied Physics Nankai University Tianjin 300071 P.R.China State Key Laboratory of Medicinal Chemical Biology Frontiers Science Center for Cell Responses College of Life Sciences Nankai University Tianjin 300071P.R.China Collaborative Innovation Center of Extreme Optics Shanxi University Taiyuan Shanxi 030006P.R.China Shenzhen Research Institute of Nankai University Shenzhen Guangdong 518083P.R.China

Actin cytoskeleton plays crucial roles in various cellular *** matrix(ECM)can modulate cell morphology by remodeling the internal *** define how geometry of ECM regulates the organization of actin cytoskeleton,we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and *** was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern,but were absent from the opening edge of V-shaped pattern,indicating that the organization of actin cytoskeleton was dependent on the mechanical ***,a secondary actin ring was observed on 50μm circular pattern while did not appear on 30μm and 40μm pattern,showing a size-dependent organization of actin ***,osteoblasts,MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern,suggesting a cell-type specificity in arrangement of actin ***,our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments.

关键词： Actin cytoskeleton photolithography micropatterning extracellular matrix

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Transparent micropatterned conductive films based on highlyordered nanowire network

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Nano Research 2024年第5期17卷 4288-4297页

作者： Xin-Ran Zhang Hai-Tao Deng Xu Zeng Yi-Lin Wang Peng Huang Xiao-Sheng Zhang School of Integrated Circuit Science and Engineering University of Electronic Science and Technology of ChinaChengdu 611731China

Transparent conductive films that are based on nanowire networks are essential to construct flexible,wearable,and even stretchable ***,large-scale precise micropatterning,especially with regard to the controllability of the organizing orientation of nanowires,is a critical ***,we proposed a liquid film rupture self-assembly approach for manufacturing transparent conductive films with microstructure arrays based on a highly ordered nanowire *** large-scale microstructure conductive films were fabricated through air-liquid interface self-assembly and liquid film rupture *** typical micropattern morphologies,including square,hexagon,circle,serpentine,etc.,were prepared to reveal the universal applicability of the proposed *** homogeneity and controllability of this approach were verified for multiple *** the assembly cycles increasing,the optical transmittance decreases *** addition,theoretical model analysis is carried out,and the analytical formula of the speed of the film moving with the surface tension and the density of the liquid film is ***,the feasibility of this approach for piezoresistive strain sensors is *** fabrication approach demonstrated a cost-effective and efficient method for precisely arranging nanowires,which is useful in transparent and wearable applications.

关键词： flexible electronics transparent conductive network micropatterning nanowires micro electromechanical systems(MEMS)

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Surface-tension-confined droplet microfluidics

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Chinese Physics B 2018年第2期27卷 29-42页

作者：陈新莲伍罕巫金波 Materials Genome Institute Shanghai University College of Science Shanghai University

This article is a concise overview about the developing microfluidic systems named surface-tension-confined droplet microfluidics （STORMs）. Different from traditional complexed droplet microfluidics which generated and confined the droplets by three-dimensional （3D） poly（dimethylsiloxane）-based microchannels, STORM systems provide two- dimensional （2D） platforms for control of droplets. STORM devices utilize surface energy, with methods such as surface chemical modification and mechanical processing, to control the movement of fluid droplets. Various STORM devices have been readily prepared, with distinct advantages over conventional droplet microfluidics, which generated and confined the droplets by 3D poly（dimethylsiloxane）-based microchannels, such as significant reduction of energy consumption neces- sary for device operation, facile or even direct introduction of droplets onto patterned surface without external driving force such as a micropump, thus increased frequency or efficiency of droplets generation of specific STORM device, among others. Thus, STORM devices can be excellent alternatives for majority areas in droplet microfluidics and irreplaceable choices in certain fields by contrast. In this review, fabrication methods or strategies, manipulation methods or mechanisms, and main applications of STORM devices are introduced.

关键词： surface-tension-confined droplet microfluidics micropatterning wettability

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Polyetheretherketone and titanium surface treatments to modify roughness and wettability–Improvement of bioactivity and antibacterial properties

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Journal of Materials Science & Technology 2021年第36期95卷 213-224页

作者： Davide Porrelli Mario Mardirossian Nicola Crapisi Marco Urban Nicola Andrea Ulian Lorenzo Bevilacqua Gianluca Turco Michele Maglione Department of Medical Surgical and Health SciencesUniversity of TriestePiazza dell'Ospitale 134129TriesteItaly

Among the materials available for implant production,titanium is the most used while polyetheretherketone(PEEK)is emerging thanks to its stability and to the mechanical properties similar to the ones of the bone *** surface properties like roughness and wettability play a paramount role in cell adhesion,cell proliferation,osteointegration and implant ***,the bacterial adhesion to the biomaterial and the biofilm formation depend on surface smoothness and *** this work,two different treatments,sandblasting and air plasma,were used to increase respectively roughness and wettability of two materials:titanium and *** effects were analyzed with profilometry and contact angle *** biological properties of the material surfaces were also investigated in terms of cell adhesion and proliferation of NIH-3T3 cells,MG63 cells and human Dental Pulp Stem ***,the ability of Staphylococcus aureus to adhere and form a viable biofilm on the samples was *** biological properties of both treatments and both materials were compared with samples of Synthegra;titanium,which underwent laser ablation to obtain a porous micropatterning,characterized by a smooth surface to discourage bacterial *** cell types used were able to adhere and proliferate on samples of the tested *** adhesion was higher on sandblasted PEEK samples for both MG63 and NIH-3T3 cell lines,on the contrary,the highest proliferation rate was observed on sandblasted titanium and was only slightly dependent on wettability;h DPSCs were able to proliferate similarly on sandblasted samples of both tested *** highest osteoblast differentiation was observed on laser micropatterned titanium samples,but similar effects,even if limited,were also observed on both sandblasted materials and air plasma treated *** lowest bacterial adhesion and biofilm formation was observed on micropatterned titanium samples whereas,the

关键词： PEEK Titanium micropatterning Roughness Wettability Differentiation

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Giant enhancement of photoluminescence emission in monolayer WS_(2)by femtosecond laser irradiation

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Frontiers of physics 2021年第1期16卷 143-150页

作者： Cheng-Bing Qin Xi-Long Liang Shuang-Ping Han Guo-Feng Zhang Rui-Yun Chen Jian-Yong Hu Lian-Tuan Xiao Suo-Tang Jia State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Laser SpectroscopyShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Monolayer transition metal dichalcogenides have emerged as promising mat erials for opt oelectTonic and nanophotonic ***,the low photoluminescence(PL)quantum yield(QY)hinders their various potential *** we engineer and enhance the PL intensity of monolayer WS_(2)by femtosecond laser *** than two orders of magnitude enhancement of PL intensity as compared to the as-prepared sample is ***,the engineering time is shortened by three orders of magnitude as compared to the improvement of PL intensity by continuous-wave laser *** on the evolution of PL spectra,we attribute the giant PL enhancement to the conversion from trion emission to exciton,as well as the improvement of the QY when exciton and trion are localized to the new-formed *** have created microstructures on the monolayer WS_(2)based on the enhancement of PL intensity,where the engineered structures can be stably stored for more than three *** flexible approach with the feature of excellent long-term storage stability is promising for applications in information storage,display technology,and opto electronic devices.

关键词： monolayers WS_(2) giant enhancement photoluminescence femtosecond laser irradiation micropatterning exciton trion quantum yield

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