检索结果-南通市图书馆

Highly precise Ti/Pt/Cr/Au thin-film temperature sensor embedded in a microfluidic device

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2021年第1期40卷 195-201页

作者： Jie-Jun Wang Tao Wang Chuan-Gui Wu Wen-Bo Luo Yao Shuai Wang-Li Zhang State Key Laboratory of Electronic Thin Films and Integrated devices University of Electronic Science and Technology of ChinaChengdu 611731China

A multilayer(Ti/Pt/Cr/Au)resistive temperature sensor was proposed and investigated to precisely measure the temperature characteristic in microfluidic *** Ti/Pt/Cr/Au sensor was fabricated by direct current(DC)sputtering,vacuum evaporation and liftoff *** thermal annealing test was conducted in the temperature range of 200-800℃for obtaining an appropriate property of the *** on the experimental results,400℃was selected as the experimental annealing temperature for the Ti/Pt/Cr/Au *** redistribution of structural imperfections and recrystallization promote the density and adhesion of multilayer during the annealing *** the annealing temperature rising,the annealing process leads to through-thickness migration of chromium and partial depletion of the adhesive *** Ti also diffuses into the Pt,which makes the interface ***,the layer remains *** temperature coefficient of resistance(TCR)of the sensors was investigated through the microfluidic testing *** excellent stability and sensitivity of the Ti/Pt/Cr/Au thin-film temperature sensor are ***,the capability of the Ti/Pt/Cr/Au thin-film temperature sensor detecting the sudden temperature change caused by bubble effect is very meaningful to the microfluidic devices.

关键词： Ti/Pt/Cr/Au layer Annealing process Recrystallization Temperature coefficient of resistance(TCR) microfluidic device

A titanium dioxide nanorod array as a high-affinity nano-bio interface of a microfluidic device for efficient capture of circulating tumor cells

在线全文

学校读者我要写书评

暂无评论

Nano Research 2017年第3期10卷 776-784页

作者： Jichuan Qiu Kun Zhao Linlin Li Xin Yu Weibo Guo Shu Wang Xiaodi Zhang Caofeng Pan Zhong Lin Wang Hong Liu Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences National Center for Nanoscience and Technology (NCNST) Beijing 100083 China Bio-Micro-Nano Functional Materials Center State Key Laboratory of Crystal Materials Shandong University 3inan 250100 China

Nanomaterials show promising opportunities to address clinical problems （such as insufficient capture of circulating tumor cells; CTCs） via the high surface area-to-volume ratio and high affinity for biological cells. However, how to apply these nanomaterials as a nano-bio interface in a microfluidic device for efficient CTC capture with high specificity remains a challenge. In the present work, we first found that a titanium dioxide （TiO2） nanorod array that can be conveniently prepared on multiple kinds of substrates has high affinity for tumor cells. Then, the TiO2 nanorod array was vertically grown on the surface of a microchannel with hexagonally patterned Si micropillars via a hydrothermal reaction, forming a new kind of a micro-nano 3D hierarchically structured microfluidic device. The vertically grown TiO2 nanorod array was used as a sensitive nano-bio interface of this 3D hierarchically structured microfluidic device, which showed high efficiency of CTC capture （76.7% ± 7.1%） in an artificial whole-blood sample.

关键词： TiO2 nanorod array circulating tumor cell microfluidic device nano-bio interface 3D hierarchical structure

Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2012年第4期55卷 537-542页

作者： ZHU DeRong LIU Xia WANG ZhenXin Department of Medicinal Chemistry and Pharmaceutical analysis Guangdong Medical CollegeDongguan 523808China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

We report a functionalisation strategy which is able to generate Ricinus communis agglutinin 1 （RCA 120） modified PMMA microfluidic device for binding and culturing living cells. The functionalisation is achieved by standard aminealdehyde （Schiff base） reaction through the crosslinker, glutaraldehyde. To prove the ability of the RCA 120 modified PMMA surface, the PC 12 cell line （rat pheochromocytoma ceils） has been captured and cultured by the microfluidic device. In the presence of tunicamycin, the dose/timedependence on decreasing of binding affinity of RCA 120 modified device with PC 12 cell is also observed. The experimental results demonstrate that the lectin-functionalized microfluidic device can be employed as efficient cell culturing platform, and has a great promise of being used as a powerful tool for monitoring the interaction of drug with living cell.

关键词： poly(methyl methacrylate) (PMMA) microfluidic device Ricinus communis agglutinin I (RCA 120) tunicamycin PC 12 cell line

A microfluidic device for accurate detection of hs-cTnI

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第4期32卷 1555-1558页

作者： Li Huang Enben Su Yuan Liu Nongyue He Yan Deng Lian Jin Zhu Chen Song Li State Key Laboratory of Bioelectronics National Demonstration Center for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Getein Biotechnology Co. Ltd.Nanjing 210000China Hunan Key Laboratory of Biomedical Nanomaterials and devices Hunan University of TechnologyZhuzhou 412007China

This device is aimed at ensuring that the sample is uniformly and equivalently reacted with the antibody on the NC membrane in each test when the microfluidic liquid system is introduced to the *** this study,the developed microfluidic chip can avoid the presence of the sample and conjugate pads in the chip,while the precision of the chro matography system can be greatly improved using the same particles,NC membrane and antibody alongside the traditional *** results,taking the detection of cTnI as an example,revealed that the coefficient of variation(CV)is controlled within 4%,while the maximum record of the contrast chromatographic reagent strip can reach 15%.Additionally,the detection sensitivity can maintain the same order of magnitudes with that of the traditional chromatographic *** the results,the determination correlation of the developed microfluidic chip has been greatly *** addition,the CV of the chip in this study is greatly improved in comparison with that of the traditional *** biggest improvement lies in the mixing between the sample and the microspheres,indicating that this is a new approach to improve the CV of the traditional strip.

关键词： Point of care testing hs-cTnI microfluidic device Precision

A light-addressable microfluidic device for label-free functional assays of bioengineered taste receptor cells via extracellular recording

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2019年第2期5卷 73-79页

作者： Liping Du Wei Chen Yulan Tian Ping Zhu Jian Wang Wen Cai Chunsheng Wu Institute of Medical Engineering Department of Biophysics School of Basic Medical Sciences Health Science Center Xi'an Jiaotong University

The functional assay of chemical sensitive cells is of great importance for the preparation of sensitive elements towards the development of cell-based biosensors for chemical sensing. In this study, a novel light-addressable microfluidic device was developed by the integration of a light-addressable potentiometric sensor(LAPS) with a microfluidic chip for label-free functional assays of bioengineered taste receptor cells via extracellular recording. Extracellular potential changes of single bioengineered cells were recorded by LAPS. microfluidic chip was capable of providing stable microenvironments for cell measurements with a well-defined concentration stimulus. Bioengineered taste receptor cells were utilized as a model of chemical sensitive cells and functional assayed by this light-addressable microfluidic device using bitter stimulation. The results indicate that this microfluidic device can efficiently monitor the membrane potential changes originated from bioengineered taste receptor cells in response to specific bitter stimulation. The bioengineered cells with responsive functions can be easily identified via the analysis on the firing rates of extracellular recording data. The expression of bitter receptors in the bioengineered taste receptor cells was further validated by the immunofluorescent staining results, which proved that the expression of specific bitter receptor was the main contribution to the responsive function of bioengineered cells. This microfluidic device can not only be used for the functional assays of chemical sensitive cells towards chemical sensing, but also suitable to be applied for the research on the chemical signal transduction mechanisms.

关键词： Cell-based biosensor Light-addressable potentiometric sensor microfluidic device Bioengineered taste receptor cell Extracellular recording

The Fabrication of the microfluidic device with Micro- and Nano- Scale Channels

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The Fabrication of the Microfluidic Device with Micro- and N...

Development of PDMS-based microfluidic device for Cell-based Assays

Mechanical Engineering and Material Science—2012 International Conference on Mechanical Engineering and Material Science(MEMS 2012)

作者： Guangchun Fu Department of Mechanical and Electrical Engineering Xiamen University China Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen University China Zezhi Zheng Department of Physics Xiamen University China Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen University ChinaYang Li Department of Materials Science & Engineering Xiamen University China Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen University China Hong Chen Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen University China

Here a simple method for fabrication of the microfluidic device with micro-and nanochannels was *** microchannels in micro and nano scales were fabricated on a glass substrate during a two-step process *** then the glass substrate with the microchannels was bonded with another flat glass substrate at room temperature to avoid the collapse of the *** UV lithography and wet etching were used in this *** nanochannels with a series of depths were obtained with optimized etchant concentration of 1.5×10 -2 mol L -1 HF,7.5×10 -3 mol L -1 HNO 3 and 7.5×10 -3 mol L -1 NH 4 F,and the depth had a linear relationship with the etching time （the square of correlation coefficient was 0.9983）.The roughness length of the etched nanochannel was 2.045 nm investigated by atomic force microscopy （AFM）.The substrate was then bonded with a flat glass plate to form a closed microchannel,the cross-section of the bonded microchannel was imaged by a scanning electron microscopy （SEM）.With this method,the microchannels combined with micro and nano scale can be prepared conveniently with low cost.

关键词： microfluidic device nanochannel

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Development of PDMS-based Microfluidic Device for Cell-based...

microfluidic thermotaxic selection of highlymotile sperm and in vitro fertilization

第二届全国微全分析系统学术会议

作者： LI Cheuk-Wing, YANG Jun, TZANG Chi-Hung, YANG Meng-Su (Applied Research Centre for Genomic Technology, and Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China )

＜正＞ In a single step photolithography, multi-level microfluidic device is fabricated by printing novel architectures on a film photomasks. The whole fabrication process is executed by classical PCB technology without the need to access clean room facilities. Different levels of protruding features on PCB master are produced by exposing a photomask with specifically arranged "windows and rims" architectures, followed by chemical wet etching. Poly（dimethylsiloxane）（PDMS） is then molded against the positive relief master to generate microfluidic device featured with multi-level sandbag structure and peripheral microchannels. This sandbag structure is an analog to traditional dam or weir for particle entrapment. The microstructure does not collapse when subjected to applied pressure, which is suitable for operation on elastic PDMS substrate. Typical immunocytochemcial staining assays were performed in the microdevice to demonstrate the applicability of the sandbag structure for cellular analysis. This simplified microfabrication process employs low-cost materials and minimal specialized equipment and can reproducibly produce mask lines with about 20 μm in width, which is sufficient for most microfluidic applications.

关键词： microfluidic device PDMS Cell-based assays

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2024年第5期7卷 687-700页

作者： Sihan Chen Jiemin Chen Zihan Qin Jibo Wang Yuwen Wang Rong Liu Wen Zhao Ming Zhang Yuanzhen Zhang Mengcheng Luo Pu Chen Tissue Engineering and Organ Manufacturing(TEOM)Lab Department of Biomedical EngineeringTaiKang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease TaiKangMedical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Reproductive Medicine Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China TaiKang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic *** adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to *** a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment *** this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is *** by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and *** study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm *** indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;Pmicrofluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.

关键词： microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology In vitro fertilization

microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism

在线全文

学校读者我要写书评

暂无评论

Cell Regeneration 2022年第1期11卷 337-347页

作者： Ning Huang Zu-Hang Sheng Synaptic Function Section The Porter Neuroscience Research CenterNational Institute of Neurological Disorders and StrokeNational Institutes of HealthRoom 2B-21535 Convent DriveBethesdaMD 20892-3706USA Department of Physiology and Pathophysiology School of Basic Medical SciencesXi’an Jiaotong University Health Science CenterXi’an 710061ShaanxiChina Institute of Neuroscience Translational Medicine InstituteXi’an Jiaotong University Health Science CenterXi’an 710061ShaanxiChina

Central nervous system(CNS)neurons typically fail to regenerate their axons after injury leading to neurological *** regeneration is a highly energy-demanding cellular program that requires local mitochondria to supply most energy within injured *** emerging lines of evidence have started to reveal that injury-triggered acute mitochondrial damage and local energy crisis contribute to the intrinsic energetic restriction that accounts for axon regeneration failure in the *** and reprogramming bioenergetic signaling and mitochondrial maintenance after axon injury-ischemia is fundamental for developing therapeutic strategies that can restore local energy metabolism and thus facilitate axon ***,establishing reliable and reproduc-ible neuronal model platforms is critical for assessing axonal energetic metabolism and regeneration capacity after *** this focused methodology article,we discuss recent advances in applying cutting-edge microflu-idic chamber devices in combination with state-of-the-art live-neuron imaging tools to monitor axonal regeneration,mitochondrial transport,bioenergetic metabolism,and local protein synthesis in response to injury-ischemic stress in mature CNS neurons.

关键词： microfluidic device Axon injury Ischemia Axon regeneration Mitochondrial transport Axonal bioenergetics Axonal protein synthesis