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Decomposition effects of Lanzhou lily(Lilium davidii ***)flowers on soil physical and chemical properties and microbial community diversity

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Research in Cold and Arid Regions 2022年第3期14卷 212-222页

作者： Jie Li YaJun Wang Yang Qiu ZhongKui Xie YuBao Zhang CuiPing Hua Northwest Institute of Eco-Environment and Resources Chinese Academy of SciencesLanzhouGansu 730000China University of Chinese Academy of Sciences Beijing 100049China

Timely removal of the flower is a key agricultural measure to ensure the concentrated supply of nutrients for the growth of underground bulbs and to increase the yield of lilies. Removing flowers and returning them to the field is one of the tradi‐tional ways of treatment, and field litter is formed at this time. Previous study showed that the decomposition of litter changes the soil properties. In order to study the effects of lily litter decomposition on soil physical and chemical proper‐ties and microbial structure, three treatments were set up in reference to the Decomposition Bag Method: control (CK), Lanzhou lily flower treatment (LZF), and Zhongbai No.1 flower treatment (ZBF). The effects of lily decomposition on soil physical and chemical properties and microbial community composition were studied in order to provide a scientific basis and theoretical guidance for the planting process of Lanzhou lily. The results show that the decomposition of lily flowers significantly increased the contents of soil organic matter, soil total nitrogen, soil total phosphorus and soil avail‐able potassium, and decreased soil pH. RDA shows that soil available nutrients and pH were the driving factors for the change of the soil microbial community. A short-term change of soil microenvironment caused by the decomposed lily flower is beneficial to growing the Lanzhou lily. However, under the correlation analysis of environmental factors, the long-term effects of returning the Lanzhou lily flower to the field, such as the trend of soil acidification, need to be further studied.

关键词： removing flower litter decomposition soil physicochemical properties microbial diversity

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How Does Heat-Stress Intensity Affect the Stability of microbial Activity and diversity of Soil microbial Communities in Outfields and Homefields’ Cultivation Practices in the Senegalese Groundnut Basin?

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Open Journal of Soil Science 2023年第2期13卷 97-123页

作者： Paul Ndiaga Ciss Laure Tall Saidou Nourou Sall Mariama Dalanda Diallo Paula Fernandes Tidiane Dieye Medoune Mbengue Espoir Gaglo Komi Assigbetse LABAAM UFR S2ATA Université Gaston Berger Saint Louis Sénégal ISRA (Institut Sénégalais de Recherche Agricole) LNRPV Dakar Sénégal IPAR (Initiative Prospective Agricole et Rurale) Dakar Sénégal LMI IESOL Centre ISRA-IRD-UCAD Dakar Sénégal CIRAD UPR Hortsys Centre ISRA-IRD-UCAD Dakar Sénégal Eco & Sols Université de Montpellier IRD CIRAD INRAE Institut Agro Montpellier France Hortsys CIRAD Univ Montpellier Montpellier France

Agroecosystems in the Senegalese groundnut basin experience long periods of high temperatures and drought, which disrupt the stability of soil microbial communities. This study evaluated how that stability is affected by homefields and outfields’ agricultural practices and the duration of heat stress. Specifically, we collected soils from organically farmed fields that receive continual high inputs of manure (homefields), and from fields that are rarely manured (outfields). Soil samples were submitted to artificial heat stress at 60°C for 3, 14, and 28 days, followed by 28 days of recovery at 28°C. We examined the functional stability of microbial communities by quantifying C mineralization, and characterized the stability of the communities’ taxonomic compositions via high-throughput DNA sequencing. We found that the microbial communities have a low resistance to heat stress in soils from both types of fields. However, the manuring practice does affect how the functional stability of microbial communities responds to different durations of heat stress. Although functional stability was not recovered fully in either soil, microbial community resilience seemed to be greater in homefield soils. Differences in manuring practices also affected the structural taxonomic stability of microbial communities: relative abundances of Bacilli, Chloroflexia, Actinobacteria and Sordariomycetes increased in the homefield stressed-soils, but decreased significantly in outfield soils. In contrast, relative abundances of α-Proteobacteria, γ-Proteobacteria and Eurotiomycetes increased significantly in outfield stressed-soils, while decreasing significantly in the homefield soils. Relative abundances of Bacilli changed little in outfield soils, indicating that this taxon is resistant to heat stress. In summary, the microbial communities’ capacities to resist heat stress and recover from it depend upon the organic richness of the soil (i.e., manuring practice) and the adaptation of soil

关键词： Groundnut Basin Senegal Agricultural Practice Heat Stress microbial Stability microbial diversity

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microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

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Acta Oceanologica Sinica 2014年第10期33卷 94-104页

作者： WU Yuehong CAO Yi WANG Chunsheng WU Min AHARON Oren XU Xuewei Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of Oceanography State Oceanic Administration State Key Laboratory of microbial Resources Institute of Microbiology Chinese Academy of Sciences College of Life Sciences Zhejiang University Department of Plant and Environmental Sciences the Alexander Silberman Institute of Life Sciences The Hebrew University of JerusalemJerusalem 91904 Israel The Hebrew University of Jerusalem The Edmond J. Safra CampusGivat Ram Jerusalem 91904 Israel

A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction（PCR） amplification of the 16 S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E（MBGE） and marine group I（MGI） belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16 S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.

关键词： deep-sea hydrothermal vent microbial diversity 16S rRNA gene nifH gene

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diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high-throughput amplicon sequencing

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Insect Science 2020年第5期27卷 883-894页

作者： Zheng-Liang Wang Tian-Zhao Wang Hang-Feng Zhu Hai-Bo Pan Xiao-Ping Yu Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine College of Life SciencesChina Jiliang UniversityHangzhouChina

The microbiome associated with brown planthopper(BPH)plays an important role in mediating host health and *** of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host ***,we investigated the bacterial and fungal communities of BPH at different developmental stages using high-throughput amplicon *** results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH *** bacterial communities were generally richer than fungi in each developmental *** 97%similarly,19 phyla and 278 genera of bacteria were an-notated,while five fungal phyla comprising 80 genera were *** highest species richness for the bacterial communities was detected in the nymphal *** taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental *** most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota,re-spectively.A significantly lower abundance of bacterial genus Acinetobacter was recorded in the egg stage when compared to other developmental stages,while the dominant fun-gal genus Wallemia was more abundant in the nymph and adult stages than in the egg ***,the microbial composition differed between male and female adults,suggesting that the microbial communties In BPH were ***,our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.

关键词： amplicon sequencing brown planthopper dynamics microbial diversity microbiome

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microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre

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Acta Oceanologica Sinica 2013年第2期32卷 42-51页

作者： WEI Manman ZHANG Rubing WANG Yuguang JI Houguo ZHENG Jia CHEN Xinhua ZHOU Hongbo School of Minerals Processing and Bioengineering Central South University Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Third Institute of Oceanography State Oceanic Administration Key Laboratory of Biometallurgy Ministry of Education

The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields＇ sediments along the Eastern Lau Spreading Centre （ELSC） in the Lau Basin （southwest Pacific）. microbial communities were surveyed by denatured gradient gel electrophoresis （DGGE） and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles sepa- rated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, repre- senting two selected hydrothermal samples （19-4TVG8 and 19-4TVG11）, were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hy- drothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.

关键词： microbial diversity deep-sea hydrothermal vent DGGE 16S rRNA gene clone libraries

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Changes in diversity,composition and assembly processes of soil microbial communities during Robinia pseudoacacia *** on the Loess Plateau,China

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Journal of Arid Land 2022年第5期14卷 561-575页

作者： WANG Kun WANG Xiaoxia FEI Hongyan WAN Chuanyu HAN Fengpeng State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100China Research Center on Soil&Water Conservation Institute of Soil and Water ConservationChinese Academy of Sciences and Ministry of Water ResourcesYangling 712100China

Robinia pseudoacacia L.(RP)restoration has increased vegetation cover in semi-arid regions on the Loess Plateau of China,but ecological problems have also occurred due to RP restoration,such as reduced soil ***,it is still uncertain how microbial diversity,composition and assembly processes change with RP restoration in semi-arid ***,amplicon sequencing of small subunit ribosomal ribonucleic acid(16S rRNA)and internal transcribed spacer(ITS)genes was performed to study soil bacterial and fungal diversity,composition and assembly processes at four study sites with different stand ages of RP plantations(Y10,RP plantation with stand ages less than 10 a;Y15,RP plantation with stand ages approximately 15 a;Y25,RP plantation with stand ages approximately 25 a;and Y40,RP plantation with stand ages approximately 40 a)along a 40-a chronosequence on the Loess *** diversity of soil bacteria and fungi increased significantly during the restoration period from 10 to 15 a(Pdiversity was lower at Y25 and Y40,and fungal diversity remained stable during the restoration period between 25 and 40 *** relative abundances of Proteobacteria and Ascomycota increased during the restoration period from 10 to 15 ***,after 15 a of restoration,they both decreased,whereas the relative abundances of Actinomycetes,Acidobacteria and Basidiomycota gradually *** variations in soil bacterial communities were mainly related to changes in soil total nitrogen,nitrate nitrogen and moisture contents,while soil fungal communities were mainly shaped by soil organic carbon and nitrate nitrogen *** communities were structured by the heterogeneous selection and stochastic process,while fungal communities were structured primarily by the stochastic *** RP restoration induced an increase in the relative importance of heterogeneous selection on bacterial ***,this study reveals t

关键词： Robinia pseudoacacia microbial diversity community structure community assembly restoration semi-arid regions

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Response of anaerobes to methyl fluoride,2-bromoethanesulfonate and hydrogen during acetate degradation

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Journal of Environmental Sciences 2013年第5期25卷 857-864页

作者： Liping Hao Fan L Lei Li Liming Shao Pinjing He State Key Laboratory of Pollution Control and Resource Reuse Tongji University Institute of Waste Treatment and Reclamation Tongji University

To use the selective inhibition method for quantitative analysis of acetate metabolism in methanogenic systems,the responses of microbial communities and metabolic activities,which were involved in anaerobic degradation of acetate,to the addition of methyl fluoride（CH3F）,2-bromoethanesulfonate（BES）and hydrogen were investigated in a thermophilic batch *** the methanogenic inhibitors,i.e.,CH3F and BES,showed their effectiveness on inhibiting CH4 production,whereas acetate metabolism other than acetoclastic methanogenesis was stimulated by BES,as reflected by the fluctuated acetate *** acetate oxidation was thermodynamically blocked by hydrogen（H2）,while H2-utilizing reactions as hydrogenotrophic methanogenesis and homoacetogenesis were correspondingly *** of PCR-DGGE fingerprinting showed that,CH3F did not influence the microbial populations ***,the BES and hydrogen notably altered the bacterial community structures and increased the *** gradually changed the methanogenic community structure by affecting the existence of different populations to different levels,whilst H2 greatly changed the abundance of different methanogenic populations,and induced growth of new species.

关键词： methanogenic inhibitor hydrogen thermophilic anaerobic digestion microbial diversity denaturing gradient gel electrophoresis(DGGE)

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Bacterial diversity in soils around a lead and zinc mine

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Journal of Environmental Sciences 2007年第1期19卷 74-79页

作者： HU Qing QI Hong-yan ZENG Jing-hai ZHANG Hong-xun Department of Environmental Biotechnology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Five samples of soil collected from a lead and zinc mine were used to assess the effect of combined contamination of heavy metals on soil bacterial communities using a polyphasic approach including characterization of isolates by culture method, community level catabolic profiling in BIOLOG GN microplates, and genetic community fingerprinting by denaturing gradient gel electrophoresis of 16S rDNA fragments amplified by PCR from community DNA （PCR-DGGE）. The structure of the bacterial community was affected to a certain extent by heavy metals. The PCR-DGGE analysis of 16S rRNA genes showed that there were significant differences in the structure of the microbial community among the soil samples, which were related to the contamination levels. The number of bacteria and the number of denaturing gradient gel electrophoresis （DGGE） bands in the soils increased with increasing distance from the lead and zinc mine tailing, whereas the concentration of lead （Pb） and cadmium （Cd） was decreased. Heavily polluted soils could be characterized by a community that differs from those of lightly polluted soils in richness and structure of dominating bacterial populations. The clustering analysis of the DGGE profiles showed that the bacteria in all the five samples of soil belonged to three clusters. The data from the BIOLOG analysis also showed the same result. This study showed that heavy metal contamination decreased both the biomass and diversity of the bacterial community in soil.

关键词： BIOLOG system DGGE heavy metal microbial diversity soils

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Characterization of depth-related microbial communities in lake sediment by denaturing gradient gel electrophoresis of amplified 16S rRNA fragments

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Journal of Environmental Sciences 2008年第2期20卷 224-230页

作者： ZHAO Xingqing YANG Liuyan YU Zhenyang PENG Naiying XIAO Lin YIN Daqiang QIN Boqiang State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China

The characterization of microbial communities of different depth sediment samples was examined by a culture-independent method and compared with physicochemical parameters, those are organic matter （OM）, total nitrogen （TN）, total phosphorus （TP）, pH and redox potential （Eh）. Total genomic DNA was extracted from samples derived from different depths. After they were amplified with the GC-341 f/907r primer sets of partial bacterial 16S rRNA genes, the products were separated by denaturing gradient gel electrophoresis （DGGE）. The profile of DGGE fingerprints of different depth sediment samples revealed that the community structure remained relatively stable along the entire 45 cm sediment core, however, principal-component analysis of DGGE patterns revealed that at greater sediment depths, successional shifts in community structure were evident. The principle coordinates analysis suggested that the bacterial communities along the sediment core could be separated into two groups, which were located 0-20 cm and 21-45 cm, respectively. The sequencing dominant bands demonstrated that the major phylogenetic groups identified by DGGE belonged to Bacillus, Bacterium, Brevibacillus, Exiguobacterium, γ-Proteobacterium, Acinetobacter sp. and some uncultured or unidentified bacteria. The results indicated the existence of highly diverse bacterial community in the lake sediment core.

关键词： 16S rRNA denaturing gradient gel electrophoresis (DGGE) Lake Taihu microbial diversity sediment vertical distribution

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Nitrogen removal from wastewater and bacterial diversity in activated sludge at different COD/N ratios and dissolved oxygen concentrations

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Journal of Environmental Sciences 2012年第6期24卷 990-998页

作者： Magdalena Zielińska Katarzyna Bernat Agnieszka Cydzik-Kwiatkowska Joanna Sobolewska Irena Wojnowska-Baryla Department of Environmental Biotechnology University of Warmia and Mazury in OlsztynS oneczna St.45G10-709 OlsztynPoland

The impact of the organic carbon to nitrogen ratio （chemical oxygen demand （COD）/N） in wastewater and dissolved oxygen （DO） concentration on carbon and nitrogen removal efficiency, and total bacteria and ammonia-oxidizing bacteria （AOB） communities in activated sludge in constantly aerated sequencing batch reactors （SBRs） was determined. At DO of 0.5 and 1.5 mg O2/L during the aeration phase, the efficiency of ammonia oxidation exceeded 90%, with nitrates as the main product. Nitrification and denitrification achieved under the same operating conditions suggested the simultaneous course of these processes. The most effective nitrogen elimination （above 50%） was obtained at the COD/N ratio of 6.8 and DO of 0.5 mg O2/L. Total bacterial diversity was similar in all experimental series, however, for both COD/N ratios of 6.8 and 0.7, higher values were observed at DO of 0.5 mg O2/L. The diversity and abundance of AOB were higher in the reactors with the COD/N ratio of 0.7 in comparison with the reactors with the COD/N of 6.8. For both COD/N ratios applied, the AOB population was not affected by oxygen concentration. Amplicons with sequences indicating membership of the genus Nitrosospira were the determinants of variable technological conditions.

关键词： nitrogen removal activated sludge COD/N ratio oxygen concentration ammonia-oxidizing bacteria microbial diversity

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