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Roles of micrornas in immunopathogenesis of non-alcoholic fatty liver disease revealed by integrated analysis of microrna and mrna expression profiles

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Hepatobiliary & Pancreatic Diseases International 2017年第1期16卷 65-79页

作者： Yu-Jun Zhang Ying Hu Jing Li Yu-Jing Chi Wei-Wei Jiang Feng Zhang Yu-Lan Liu institute of clinical molecular biology peking university people’s hospital Beiiing 100044 China department of gastroenterology peking university people’s hospital Beiiing 100044 China

BACKGROUND： The integrative analysis of microrna and mrna expression profiles can elucidate microrna-targeted gene function. We used this technique to elucidate insights into the immunological pathology of non-alcoholic fatty liver disease （NAFLD）. METHODS： We analyzed differentially expressed microrna and mrna expression profiles of CD4＋ T lymphocytes from the liver and mesenteric lymph nodes （MLNs） of mice with NAFLD using microarrays and RNA sequencing. Normal mice were used as controls. The target genes of micrornas were predicted by TargetScan. Integrative analysis showed that the mrnas were overlapped with micrornas. Furthermore, the Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analyses were performed to predict the key genes and pathways. Then, 16 micrornas and 10 mrnas were validated by qRT-PCR. RESULTS： Microarray analysis suggested that 170 micrornas were significantly de-regulated in CD4＋ T lymphocytes from the liver between the two groups. Eighty mrnas corresponded with microrna targeted genes. KEGG analysis indicated that the MAPK pathway was consistently augmented in the liver of NAFLD mice. miR-23b, let-7e, miR-128 and miR-130b possibly played significant parts in the MAPK pathways. Furthermore, between the two groups, 237 micrornas were significantly deregulated in CD4＋ T lymphocytes from MLNs. 38 mrnas coincided with microrna target genes. The metabolic pathway was consistently enriched in the MLNs of NAFLD mice. miR- 206-3p, miR-181a-Sp, miR-29c-3p and miR-30d-5p likely play important roles in the regulation of metabolic pathways. CONCLUSION： The results of this study presented a new perspective on the application of integrative analysis to identify complex regulation means involved in the immunological pathogenesis of NAFLD.

关键词： microrna-mrna gut non-alcoholic fatty liver disease CD4+ T lymphocytes immunopathogenesis

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Integrated analysis of miRNA and mrna expression profiles in response to gut microbiota depletion in the abdomens of female Bactrocera dorsalis

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Insect Science 2023年第2期30卷 443-458页

作者： JunfeiXie Zhaohui Cai Wenping Zheng Hongyu Zhang Key Laboratory of Horticultural Plant Biology(MOE) Hubei Hongshan LaboratoryChina-Australia Joint Research Centre for Horticultural and Urban PestsInstitute of Urban and Horticultural EntomologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China

Insect gut microbiota has been reported to participate in regulating host multiple biological processes including metabolism and ***,the corresponding molecular mechanisms remain largely *** studies suggest that micrornas(miRNAs)are involved in complex interactions between the gut microbiota and the ***,we used next-generation sequencing technology to characterize miRNA and mrna expression profiles and construct the miRNA–gene regulatory network in response to gut microbiota depletion in the abdomens of female Bactrocera dorsalis.A total of 3016 differentially expressed genes(DEGs)and 18 differentially expressed miRNAs(DEMs)were *** on the integrated analysis of miRNA and mrna sequencing data,229 negatively correlated miRNA-gene pairs were identified from the miRNA-mrna *** ontology enrichment analysis indicated that DEMs could target several genes involved in the metabolic process,oxidation-reduction process,oogenesis,and insulin signaling ***,real-time quantitative polymerase chain reaction further verified the accuracy of RNA sequencing *** conclusion,our study provides the profiles of miRNA and mrna expressions under antibiotics treatment and provides an insight into the roles of miRNAs and their target genes in the interaction between the gut microbiota and its host.

关键词： Bactrocera dorsalis gut microbiota microrna-mrna regulatory pathways

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