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micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

Science China Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2, ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2Department of Chemistry, Tsinghua University, Beijing 100084, China 3Beijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing 100094, China 4School of Chemistry and Chemical Engineering, Xianyang Normal College, Xianyang 712000, China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China Department of Chemistry Tsinghua University Beijing China Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing China School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

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ON THE STABILITY OF A FUNCTIONALLY GRADED RECTANGULAR micro-plate SUBJECTED TO HYDROSTATIC AND NONLINEAR ELECTROSTATIC PRESSURES

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Acta Mechanica Solida Sinica 2013年第2期26卷 205-220页

作者： Adel Nabian Ghader Rezazadeh Morteza Almassi Ali-Mohammad Borgheei Department of Agricultural Machinery Engineering Science and Research BranchIslamic Azad UniversityTehranIran Department of Mechanical Engineering Urmia UniversityUrmiaIran

This article studies the stability of a functionally graded clamped-clamped micro- plate subjected to hydrostatic and electrostatic pressures. Equilibrium positions of the micro-plate are determined and shown in the state control space. To study the stability of the equilibrium positions, the motion trajectories are given for different initial conditions in the phase plane. Effects of the electrostatic and hydrostatic pressure changes on the deflection and stability of the micro-plate for some sample value of k are studied and values of the applied voltage and hydrostatic pressure leading system to unstable conditions by undergoing a saddle node and homoclinic bifurcations are determined.

关键词： MEMS FGM stability micro-plate electrostatic hydrostatic pressure

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A Monolithic, FEM-Based Approach for the Coupled Squeeze Film Problem of an Oscillating Elastic micro-plate Using 3D 27-Node Elements

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Journal of Applied Mathematics and Physics 2013年第6期1卷 20-25页

作者： Anish Roychowdhury Arup Nandy C. S. Jog Rudra Pratap 1Department of Mechanical Engineering Indian Institute of Science Bangalore India 2Center for Nano Science and Engineering Indian Institute of Science Bangalore India Department of Mechanical Engineering Indian Institute of Science Bangalore India

In this study we describe an FEM-based methodology to solve the coupled fluid-structure problem due to squeeze film effects present in vibratory MEMS devices, such as resonators, gyroscopes, and acoustic transducers. The aforementioned devices often consist of a plate-like structure that vibrates normal to a fixed substrate, and is generally not perfectly vacuum packed. This results in a thin film of air being sandwiched between the moving plate and the fixed substrate, which behaves like a squeeze film offering both stiffness and damping. Typically, such structures are actuated electro-statically, necessitating the thin air gap for improving the efficiency of actuation and the sensitivity of detection. To accurately model these devices the squeeze film effect must be incorporated. Extensive literature is present on mod- eling squeeze film effects for rigid motion for both perforated as well as non-perforated plates. Studies which model the plate elasticity often use approximate mode shapes as input to the 2D Reynolds Equation. Recent works which try to solve the coupled fluid elasticity problem, report iterative FEM-based solution strategies for the 2D Reynolds Equation coupled with the 3D elasticity Equation. In this work we present a FEM-based single step solution for the coupled problem at hand, using only one type of element (27 node 3D brick). The structure is modeled with 27 node brick elements of which the lowest layer of nodes is also treated as the fluid domain (2D) and the integrals over fluid domain are evaluated for these nodes only. We also apply an electrostatic loading to our model by considering an equivalent electro-static pressure load on the top surface of the structure. Thus we solve the coupled 2D-fluid-3D-structure problem in a single step, using only one element type. The FEM results show good agreement with both existing analytical solutions and published experimental data.

关键词： Squeeze Film Damping Coupled Problem 27-Node Brick micro-plate

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