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Polydopamine and collagen coated micro-grated polydimethylsiloxane for human mesenchymal stem cell culture

Bioactive Materials 2019年第1期4卷 142-150页

作者： Dhavan Sharma Wenkai Jia Fei Long Shweta Pati Qinghui Chen Yibing Qyang Bruce Lee Chang Kyong Choi Feng Zhao Department of Biomedical Engineering Michigan Technological University1400 Townsend DriveHoughtonMI49931USA Department of Mechanical Engineering-Engineering Mechanics Michigan Technological University1400 Townsend DriveHoughtonMI49931USA Department of Kinesiology and Integrative Physiology Department of Kinesiology and Integrative PhysiologyHoughtonMI49931USA Yale Stem Cell Center Yale University School of Medicine300 George StreetNew HavenCT 06511USA

Natural tissues contain highly organized cellular *** of the major challenges in tissue engineering is to develop engineered tissue constructs that promote cellular growth in physiological *** address this issue,micro-patterned polydimethylsiloxane(PDMS)substrates have been widely used in cell sheet engineering due to their low microfabrication cost,higher stability,excellent biocompatibility,and most importantly,ability to guide cellular growth and ***,the current methods for PDMS surface modification either require a complicated procedure or generate a non-uniform surface coating,leading to the production of poor-quality cell layers.A simple and efficient surface coating method is critically needed to improve the uniformity and quality of the generated cell ***,a fast,simple and inexpensive surface coating method was analyzed for its ability to uniformly coat polydopamine(PD)with or without collagen on micro-grated PDMS substrates without altering essential surface topographical *** feature,stiffness and cytotoxicity of these PD and/or collagen based surface coatings were further *** showed that the PD-based coating method facilitated aligned and uniform cell growth,therefore holds great promise for cell sheet engineering as well as completely biological tissue biomanufacturing.

关键词： Polydimethylsiloxane Polydopamine Collagen micro-grating Human mesenchymal stem cell

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Fabrication of micrometre-sized periodic gratings in free-standing metallic foils for laser-plasma experiments

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High Power Laser Science and Engineering 2022年第1期10卷 17-31页

作者： C.C.Gheorghiu M.Cerchez E.Aktan R.Prasad F.Yilmaz N.Yilmaz D.Popa O.Willi V.Leca Extreme Light Infrastructure-Nuclear Physics Horia Hulubei National Institute for Physics and Nuclear Engineering077125 MagureleRomania Institut fur Laser-und Plasmaphysik Heinrich-Heine-Universitat40225 DiisseldorfGermany

Engineered targets are expected to play a key role in future high-power laser experiments calling for joined, extensive knowledge in materials properties, engineering techniques and plasma physics. In this work, we propose a novel patterning procedure of self-supported 10 μm thick Au and Cu foils for obtaining micrometre-sized periodic gratings as targets for high-power laser applications. Accessible techniques were considered, by using cold rolling, electronbeam lithography and the Ar-ion milling process. The developed patterning procedure allows efficient control of the grating and foil surface on large area. Targets consisting of patterned regions of 450 μm × 450 μm, with 2 μm periodic gratings, were prepared on 25 mm × 25 mm Au and Cu free-standing foils, and preliminary investigations of the microtargets interacting with an ultrashort, relativistic laser pulse were performed. These test experiments demonstrated that,in certain conditions, the micro-gratings show enhanced laser energy absorption and higher efficiency in accelerating charge particle beams compared with planar thin foils of similar thickness.

关键词： laser driven plasmas on structured targets metallic foils micro-grating patterned targets

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