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Correlation of plasma mir-21 and mir-93 with radiotherapy and chemotherapy efficacy and prognosis in patients with esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2019年第37期25卷 5604-5618页

作者： Wen-Tao Wang Chang-Qing Guo Guang-Hui Cui Song Zhao Department of Thoracic Surgery First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina Digestive Department First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000Henan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC)is one of the main causes of human *** is usually already in middle or advanced stage when diagnosed due to its hidden symptoms in early ***,patients have already lost the best surgical timing when *** and chemotherapy are standard treatment methods for ESCC clinically,but the efficacy and prognosis of patients from them are still ***,it is of great clinical significance to seek for biomarkers that can predict the radiotherapy and chemotherapy response and prognosis of ESCC *** To explore the clinical value of plasma mir-21 and mir-93 in *** A total of 128 ESCC patients admitted to the First Affiliated Hospital of Zhenzhou University were enrolled as a study group and treated with concurrent radiotherapy and chemotherapy,and other 45 healthy people during the same period were enrolled as a control *** expression of plasma mir-21 and mir-93 was determined using quantitative real-time polymerase chain reaction,and the correlation of expression of plasma mir-21 and mir-93 with clinical pathological parameters about the patients was *** receiver operating characteristic(ROC)curve was adopted to assess the diagnostic value of plasma mir-21 and mir-93 for clinical pathological features of ESCC patients,the Logistic regression analysis adopted to analyze the risk factors for radiotherapy and chemotherapy efficacy in ESCC patients,and the Cox regression analysis to identify the prognostic factors for ESCC *** The study group showed significantly higher relative expression of plasma mir-21 and mir-93 than the control group(Pmir-21 for diagnosing T stage,N stage,M stage,and pathological differentiation of ESCC was 0.819,0.758,0.824,and 0.725,respectively,and that of plasma mir-93 for diagnosing T stage,N stage,and M stage of ESCC was 0.827,0.815,and 0.814,*** AUC

关键词： mir-21 mir-93 Esophageal squamous cell carcinoma Radiotherapy and chemotherapy Prognosis Diagnosis

Propofol inhibits cells migration and invasion via HOTAIR/mir-93/HIF-1α-mediated lactate secretion in colon cancer

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BIOCELL 2021年第6期45卷 1585-1599页

作者： RUONAN GU WENJING GUO WENYANG WANG GUIHUAN LI XIAOJU LAI ZHIBIN HUANG WANLU ZHAO ZHICONGWU HUA CHEN WENYANG LUO FANGYIN ZENG FAN DENG Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou510515China Department of Anesthesiology Zhujiang HospitalSouthern Medical UniversityGuangzhou510282China Department of Clinical Laboratory The Fifth Affiliated HospitalSouthern Medical UniversityGuangzhou510920China

Propofol,a common intravenous anesthetic used in clinical,has been shown to regulate cells proliferation,inflammation,angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of ***,the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain *** primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer(CRC).Scratches assay,Transwell assay were used to detect colon cells migration and *** Quantitative Polymerase Chain Reaction(RT-qPCR),Western blotting were utilized to detect the expression of related ***-luciferase assay was performed to identify transcriptional activity of target genes,RNA pulldown assay was used to confirm the interactions of *** indicated that propofol inhibited cell migration and invasion through suppressing lactic acid production in ***,propofol reduced lactic acid production by down-regulating the expression of hypoxia inducible factor-1α(HIF-1α)and monocarboxylate transporter 4(MCT4).In addition,propofol inhibited the expression of HIF-1αand cells migration/invasion via suppressing the interaction of HOX transcript antisense RNA(HOTAIR)with *** present study indicated that propofol inhibited colon cancer cell migration and invasion by reducing lactic acid production and the expression of HIF-1αand MCT4 via HOTAIR/mir-93 *** data may provide potential metabolic targets for CRC treatment.

关键词： Propofol Colon cancer Lactic acid HIF-1α HOTAIR mir-93

mir-93 suppresses proliferation and colony formation of human colon cancer stem cells

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World Journal of Gastroenterology 2011年第42期17卷 4711-4717页

作者： Xiao-Feng Yu Jian Zou Zhi-Jun Bao Jie Dong Department of Gastroenterology Huadong Hospital Fudan University Shanghai 200040 China

AIM： TO identify differentially expressed microRNAs （mirNAs） in human colon cancer stem cells （SW1116csc） and study their function in SW1116csc proliferation. METHODS： SW1116csc were isolated from the human colon cancer cell line, SW1116 and cultured in serum- free medium. A mirNA microarray was used to detect differential expression profiles of rniRNAs in SW1116csc and SW1116 cells. Real-time quantitative polymerase chain reaction （PCR） was performed to verify the dif- ferential expression of candidate mirNAs obtained from the microarray. Target mRNAs of differentially expressed mirNAs were predicted with target predic- tion tools, mirNA expression plasmids were transfected into SW1116csc using Lipofectamine 2000 reagent. Cell proliferation curves were generated with trypan blue staining, and the colony formation rate of transfected cells was measured with the soft agar colony formation assay. Expression of target mRNAs and proteins from differentially expressed mirNAs were detected using reverse transcription （RT）-PCR and western ***： Compared with expression in SW1116 cells, 35 mirNAs （including hsa-mir-192, hsa-mir-29b, hsa-mir-215, hsa-mir-194, hsa-mir-33a and hsa- mir-32） were upregulated more than 1.5-fold, and 11 mirNAs （including hsa-mir-93, hsa-mir-1231, hsa- mirPlus-F1080, hsa-mir-524-3p, hsa-mir-886-3p and hsa-mir-561） were downregulated in SW1116csc. The mirNA microarray results were further validated with quantitative RT-PCR. mir-93 was downregulated, and its predicted mRNA targets included BAMBI, CCND2, CDKNIA, HDACS, KIF23, MAP3K9, MAP3K11, MYCN, PPARD, TLE4 and ZDHHCl. Overexpressed mir-93 sig- nificantly inhibited cell proliferation and colony forma- tion by SW1116csc. Furthermore, mir-93 negatively regulated the mRNA and protein levels of HDAC8 and TLE4. CONCLUSION： Some mirNAs were differentially ex- pressed during differentiation of SW1116csc into SW1116 cells, mir-93 may inhibit SW1116csc proliferation and colony formation.

关键词： mir-93 Stem cell Colon cancer Expres-sion profile

mir-93表达与甲状腺腺瘤手术应激胰岛素抵抗患者的关联性

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上海交通大学学报（医学版） 2014年第12期34卷 1749-1752页

作者：卢冠铭潘运龙李震东浦涧李天资梁烨马燕飞罗志斋陈永诚李近都暨南大学附属第一医院胃肠外科广州510630 右江民族医学院临床学院腺体外科百色533000

目的探讨mir-93表达与甲状腺腺瘤(TA)手术应激胰岛素抵抗(EIR)患者的关联性。方法采用氧化酶法检测32例TA合并EIR(实验组)患者总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)和空腹血清胰岛素(FINS),用qRT-PCR检测mir-93表达水平,并与32例... 详细信息

目的探讨mir-93表达与甲状腺腺瘤(TA)手术应激胰岛素抵抗(EIR)患者的关联性。方法采用氧化酶法检测32例TA合并EIR(实验组)患者总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)和空腹血清胰岛素(FINS),用qRT-PCR检测mir-93表达水平,并与32例单纯TA组(对照组)和30例健康体检者(健康组)进行比较。结果实验组TC、TG、FBG、FINS和mir-93水平分别为(6.4±0.6)mmol/L、(2.4±1.0)mmol/L、(6.3±0.5)mmol/L、(36.2±12.6)MIU/L和1.83±0.61;对照组分别为(6.3±0.6)mmol/L、(1.9±1.0)mmol/L、(6.0±0.5)mmol/L、(20.5±10.3)MIU/L和2.95±0.49;健康组分别为(5.1±0.7)mmol/L、(1.4±0.7)mmol/L、(5.1±0.6)mmol/L、(17.7±9.1)MIU/L和3.44±0.42。实验组和对照组患者治疗前mir-93表达水平分别为1.82±0.62和2.93±0.49,实验组TG、FBG和FINS水平高于对照组(Pmir-93水平低于对照组(Pmir-93表达水平差异无统计学意义(P>0.05),mir-93表达水平与TC、TG、FBG和FINS呈负相关。结论 mir-93在TA患者呈低表达,在EIR患者也呈低表达,mir-93表达水平与TC、TG、FBG和FINS呈负相关;mir-93作为保护性因素而不是致病因素参与TA手术EIR的发生和发展。

关键词：甲状腺腺瘤应激胰岛素抵抗 microRNA mir-93

mir-93通过靶定PTEN基因增加肝癌细胞对奥沙利铂的耐药性

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中国生物化学与分子生物学报 2012年第10期28卷 926-934页

作者：杨涛郑志敏张献波李振符张国栓石家庄市第一医院石家庄050011

微小RNA(mirNA)参与了肿瘤的耐药过程.本研究通过建立对奥沙利铂(oxaliplatin,Oxa)耐药的肝癌细胞系BEL-7402/Oxa和Hep-3B/Oxa,利用mirNA芯片结合实时荧光定量PCR的方法,筛选得到数个参与肝癌细胞对奥沙利铂耐药的mirNA分子,其中mir-93... 详细信息

微小RNA(mirNA)参与了肿瘤的耐药过程.本研究通过建立对奥沙利铂(oxaliplatin,Oxa)耐药的肝癌细胞系BEL-7402/Oxa和Hep-3B/Oxa,利用mirNA芯片结合实时荧光定量PCR的方法,筛选得到数个参与肝癌细胞对奥沙利铂耐药的mirNA分子,其中mir-93表达增加最为明显.MTT实验发现,增加肝癌细胞株中mir-93的表达可以增强其对奥沙利铂的耐药性.进一步结合生物信息学、荧光报告载体及免疫印迹实验,证实mir-93通过靶定抑癌基因PTEN增加肝癌细胞对奥沙利铂的耐药性.总之,肝癌耐药细胞系的建立及其mirNA差异表达谱的分析,以及mirNA分子对肝癌细胞发生奥沙利铂耐药的具体作用及其分子机制的研究,不仅有助于理解肝癌细胞发生耐药的分子机制,而且为探索克服肝癌对奥沙利铂耐药性的有效途径提供可靠依据.

关键词：微小RNA 肝癌奥沙利铂耐药 mir-93

mir-93通过靶向EphA4激活ERK通路促进非小细胞肺癌细胞的增殖和迁移

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中国肿瘤生物治疗杂志 2020年第4期27卷 370-376页

作者：洪秋双王琳娄加陶上海交通大学附属胸科医院检验科上海200030

目的:探讨mir-93/Eph受体A4(EphA4)分子轴通过细胞外调节蛋白激酶(extracellular regulated protein kinases),ERK通路对非小细胞肺癌(non-small cell lung cancer,NSCLC)H460和H1299细胞增殖和迁移的影响。方法:用qPCR检测H460和H1299细胞中mir-93表达水平。分别在H460细胞中转染mir-93模拟物(mimics)和EphA4过表达质粒、在H1299细胞中转染mir-93抑制剂(inhibitor)后,用MTT、Transwell实验检测mir-93对转染细胞增殖和迁移的影响。用双荧光素酶报告基因实验验证mir-93与EphA4之间的靶向调控关系。用Western blotting检测细胞中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、EphA4、ERK和p-ERK蛋白的表达水平,用MTT、Transwell实验检测同时过表达mir-93和EphA4对H460细胞增殖和迁移的影响。结果:mir-93在H1299细胞中表达水平高于H460细胞(Pmir-93促进H460细胞增殖和迁移(均Pmir-93抑制H1299细胞增殖和迁移(均Pmir-93靶向调控EphA4,过表达mir-93明显下调H460细胞中EphA4 mRNA和蛋白表达水平(均Pmir-93通过靶向EphA4并激活ERK通路促进H460细胞的增殖和迁移(均Pmir-93促进NSCLC细胞增殖和迁移,其机制可能与靶向调控EphA4并激活ERK通路有关。

关键词：非小细胞肺癌 H460细胞 H1299细胞 mir-93 EphA4 ERK通路增殖迁移

mir-93反义核苷酸对乳腺癌细胞增殖及凋亡的影响

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重庆医科大学学报 2014年第3期39卷 396-400页

作者：骆萍钟晓鸣谢春伟南昌市第三医院乳腺外科南昌330009 江西省肿瘤医院乳腺外科南昌330029

目的:研究mir-93在乳腺癌组织中的表达情况,并分析mir-93反义核苷酸(antisense oligonucleotide,ASO)对乳腺癌细胞增殖和凋亡的影响。方法:运用荧光定量PCR定量检测120例乳腺癌组织及对应癌旁组织中mir-93的表达;通过mir-93ASO降低乳腺... 详细信息

目的:研究mir-93在乳腺癌组织中的表达情况,并分析mir-93反义核苷酸(antisense oligonucleotide,ASO)对乳腺癌细胞增殖和凋亡的影响。方法:运用荧光定量PCR定量检测120例乳腺癌组织及对应癌旁组织中mir-93的表达;通过mir-93ASO降低乳腺癌细胞中mir-93的表达,利用MTT、细胞克隆形成实验、流式细胞仪观察mir-93 ASO对乳腺癌细胞产生的生物学效应。实验分为3组:空白对照组,无义干扰组和mir-93 ASO组。结果:在120例乳腺癌病例中,63.33%(76/120)的乳腺癌组织mir-93表达明显高于对应癌旁组织(Pmir-93 ASO可以显著降低mir-93的表达(Pmir-93 ASO 24、48 h和96 h后的细胞存活数量均明显低于空白对照组和无义干扰组(Pmir-93 ASO组克隆形成率比空白对照组和无义干扰组显著降低(Pmir-93ASO组较2个对照组细胞凋亡指数明显增加(Pmir-93的表达后发现Bcl2的mRNA和蛋白均明显下降(Pmir-93在乳腺癌组织中表达上调,降低mir-93的表达可有效抑制乳腺癌细胞生长、促进细胞凋亡。mir-93有可能成为乳腺癌基因表达调控的新靶点。

关键词： mir-93 反义核苷酸乳腺癌细胞增殖细胞凋亡

mir-93对延边黄牛垂体细胞GH分泌的影响

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中国畜牧兽医 2020年第1期47卷 116-123页

作者：娄安钢崔长艳相思宇金太花陈莹莹张睿关立增延边大学农学院延吉133002 临沂大学农林科学学院临沂276005

为了分析血液外泌体mirNA对延边黄牛垂体细胞生长激素(GH)分泌的影响,试验选择在延边黄牛和韩延牛血液外泌体中存在显著差异表达的mir-93,分析其对延边黄牛垂体细胞GH分泌的影响机制。试验首先进行延边黄牛垂体细胞的原代培养,之后将mir... 详细信息

为了分析血液外泌体mirNA对延边黄牛垂体细胞生长激素(GH)分泌的影响,试验选择在延边黄牛和韩延牛血液外泌体中存在显著差异表达的mir-93,分析其对延边黄牛垂体细胞GH分泌的影响机制。试验首先进行延边黄牛垂体细胞的原代培养,之后将mir-93的mimics(mir-93-mi组)、mimics对照品(NC对照组)、inhibitor(mir-93-in组)、inhibitor对照品(iNC组)转染给已建立的垂体原代细胞,48 h后收集细胞,提取总mRNA和总蛋白。试验利用targetscan和RNAhybrid分析软件对mir-93的靶基因进行预测,并利用双荧光素酶报告基因系统对mir-93的靶关系进行验证;利用实时荧光定量PCR和Western blotting技术分别检测靶基因mRNA转录和蛋白的表达情况,结果表明,mir-93靶向了生长激素释放激素受体(GHRHR)的3′UTR;与NC对照组比较,mir-93-mi组的延边黄牛垂体细胞中GH mRNA转录和蛋白表达均极显著低于NC对照组(Pmir-93-mi组的GHRH mRNA转录和蛋白表达均极显著低于NC对照组(Pmir-93-in组的GHRHR蛋白表达显著高于iNC对照组(Pmir-93可通过调节GHRHR的表达而调控延边黄牛垂体细胞GH的分泌,进而调控延边黄牛的生长发育。

关键词： mir-93 延边黄牛垂体细胞生长激素(GH) 生长激素释放激素受体(GHRHR)

mir-93及mir-21在糖尿病视网膜病变患者中的表达及其临床价值

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眼科新进展 2017年第12期37卷 1161-1164页

作者：符春艳海南西部中心医院眼科海南省儋州市571799

目的探讨血浆微小核糖核酸(micro RNA,mi R)-93及mi R-21在糖尿病视网膜病变(diabetic retinopathy,DR)患者中的表达及其临床价值。方法采用实时荧光定量PCR检测98例DR患者、40例2型糖尿病(type 2 diabetes mellitus,DM)患者和40名健康... 详细信息

目的探讨血浆微小核糖核酸(micro RNA,mi R)-93及mi R-21在糖尿病视网膜病变(diabetic retinopathy,DR)患者中的表达及其临床价值。方法采用实时荧光定量PCR检测98例DR患者、40例2型糖尿病(type 2 diabetes mellitus,DM)患者和40名健康体检者(对照组)血浆中mi R-93及mi R-21的表达水平。应用ROC曲线评价血浆mi R-93、mi R-21及两项联合对DR的诊断价值,多因素logistic回归分析DR发生的危险因素。Pearson相关分析DR患者血浆mi R-93、mi R-21与各生化指标的相关性。结果 DR组和DM组的尿白蛋白/肌酐、糖化血红蛋白及空腹血糖均明显高于对照组(均为Pmir-93及mir-21表达水平(1.84±0.27、1.95±0.34)均明显高于DM组(1.09±0.13、1.13±0.18)和对照组(1.02±0.08、1.08±0.16),差异均有统计学意义(均为Pmir-93及mir-21表达水平(2.31±0.57、2.28±0.61)均明显高于NPDR组(1.40±0.21、1.52±0.24),差异均有统计学意义(均为Pmir-93、mir-21及两项联合诊断DR的曲线下面积(95%CI)分别为0.892(0.824~0.961)、0.836(0.770~0.905)、0.937(0.868~0.992)。尿白蛋白/肌酐、糖化血红蛋白、空腹血糖、mir-93及mir-21是DR发生的的独立危险因素。DR患者血浆mir-93、mir-21与尿白蛋白/肌酐、糖化血红蛋白、空腹血糖均呈正相关(均为Pmir-93与mir-21呈正相关(Pmir-93和mir-21与DR的发生发展密切相关,可作为DR诊断的生物学标志物。

关键词：糖尿病视网膜病变 mir-93 mir-21 临床价值