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mi r-192-5p regulates lipid synthesis in non-alcoholic fatty liver disease through SCD-1

World Journal of Gastroenterology 2017年第46期23卷 8140-8151页

作者： Xiao-Lin Liu Hai-Xia Cao Bao-Can Wang Feng-Zhi Xin rui-Nan Zhang Da Zhou rui-Xu Yang Ze-Hua Zhao Qin pan Jian-Gao Fan Center for Fatty Liver Department of GastroenterologyXinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

AIM To evaluate the levels of mi r-192-5 p in non-alcoholic fatty liver disease(NAFLD) models and demonstrate the role of mi r-192-5 p in lipid accumulation. METHODS Thirty Sprague Dawley rats were randomly divided into three groups, which were given a standard diet, a high-fat diet(HFD), and an HFD with injection of liraglutide. At the end of 16 weeks, hepatic mi r-192-5 p and stearoyl-Co A desaturase 1(SCD-1) levels were measured. mi r-192-5 p mimic and inhibitor and SCD-1 si rNA were transfected into Huh7 cells exposed to palmitic acid(pA). Lipid accumulation was evaluated by oil red O staining and triglyceride assays. Direct interaction was validated by dual-luciferase reporter gene *** The HFD rats showed a 0.46-fold decrease and a 3.5-fold increase in hepatic mi r-192-5 p and SCD-1 protein levels compared with controls, respectively, which could be reversed after disease remission by liraglutide injection(p posed to pA also showed down-regulation and up-regulation of mi r-192-5 p and SCD-1 protein levels, respectively(p ransfection with mi r-192-5 p mimic and inhibitor in Huh7 cells induced dramatic repression and promotion of SCD-1 protein levels, respectively(p rase activity was suppressed and enhanced by mi r-192-5 p mimic and inhibitor, respectively, in wild-type SCD-1(p mi r-192-5 p overexpression reduced lipid accumulation significantly in pA-treated Huh7 cells, and SCD-1 si rNA transfection abrogated the lipid deposition aggravated by mi r-192-5 p inhibitor(p rates that mi r-192-5 p has a negative regulatory role in lipid synthesis, which is mediated through its direct regulation of SCD-1.

关键词： mi r-192-5p Stearoyl-Co A desaturase 1 High fat diet Lipid synthesis Non-alcoholic fatty liver disease

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