检索结果-南通市图书馆

轻兵器 2022年第11期 80-82页

作者：周辉邹涛王威不详

从1980年代开始,装备mi主战坦克的美军在多次对外大规模军事行动中以其机动性好、火力精准强悍等优势而闻名世界……漫画吧在此为您展示美军M1主战坦克的各种战术——。

关键词：军事行动主战坦克机动性 mi 战术

番茄Ty-1、Ty-3和mi基因的多重PCR体系建立及应用

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2018年第14期16卷 4671-4677页

作者：王明琦邰连赛陈火英刘杨王新华上海交通大学农业与生物学院上海200240 上海富农种业有限公司上海201209

本研究利用10份番茄材料,筛选出能够鉴定番茄抗黄化曲叶病基因Ty-1的双SNP标记,Ty-3基因的SCAR1标记和抗根结线虫病mi基因的SCAR2标记。应用以上分子标记构建出同时检测番茄Ty-1、Ty-3和mi基因的多重PCR体系。结果表明,多重PCR扩增结果... 详细信息

本研究利用10份番茄材料,筛选出能够鉴定番茄抗黄化曲叶病基因Ty-1的双SNP标记,Ty-3基因的SCAR1标记和抗根结线虫病mi基因的SCAR2标记。应用以上分子标记构建出同时检测番茄Ty-1、Ty-3和mi基因的多重PCR体系。结果表明,多重PCR扩增结果与单引物PCR扩增结果一致,使用该体系只需1次PCR反应及琼脂糖凝胶电泳便可快速、准确地对番茄3种基因的基因型进行检测。对96份番茄材料的分子检测结果与田间鉴定结果吻合度达到94%以上。使用该体系无需PCR产物酶切反应,可大大缩短分子检测的时间,节省检测成本,检测结果能够有效地辅助番茄抗病育种工作。

关键词：番茄 Ty-1 Ty-3 mi 多重PCR

mi R-9a-5p regulates proliferation and migration of hepatic stellate cells under pressure through inhibition of Sirt1

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第34期21卷 9900-9915页

作者： Feng Qi Jiang-Feng Hu Bao-Hai Liu Chao-Qun Wu Hong-Yu Yu Ding-Kang Yao Liang Zhu Department of Gastroenterology Changzheng Hospitalthe Second Military Medical University Department of Gastroenterology Institute of Digestive DiseasesTongji HospitalTongji University Genetics Institute Fudan University

AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal ***:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the pressureinduced mi RNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of mi RNAs. A potential target of mi R-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under ***:According to the profile,the expression of mi R-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs(3.70 ± 0.61 vs 0.97 ± 0.15,P = 0.0226),which resulted in the proliferation,migration and activation of HSCs. In vivo,the up-regulation of mi R-9a-5p(2.09 ± 0.91 vs 4.27 ± 1.74,P = 0.0025) and the down-regulation of Sirt1(2.41 ± 0.51 vs 1.13 ± 0.11,P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of mi R-9a-5p. Through restoringthe expression of Sirt1 in mi R-9a-5p transfected HSCs on pressure overload,we found that overexpression of Sirt1 could partially abrogate the mi R-9a-5p mediated suppression of the proliferation,migration and activation of HSCs. CONCLUSION:Our results suggest that during liver fibrosis,portal hypertension may induce the proliferation,migration and activation of HSCs through the up-regulation of mi R-9a-5p,which targets Sirt1.

关键词： micro RNA mi R-9a-5p Sirt1 Pressure Hepatic stella

基于DEA-mi模型的资源枯竭型城市旅游业效率评价研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

宏观经济研究 2015年第9期 117-126页

作者：张大鹏邓爱民李莺莉中南财经政法大学工商管理学院

本文以旅游景区吸引力、星级酒店数量和旅游从业人数为投入变量,以旅游收入和旅游人次为产出变量,利用2009—2014年的数据测算了全国24个资源枯竭型城市旅游业效率,从静态和动态两个方面分析了旅游业效率情况。研究发现:我国资源枯竭型... 详细信息

本文以旅游景区吸引力、星级酒店数量和旅游从业人数为投入变量,以旅游收入和旅游人次为产出变量,利用2009—2014年的数据测算了全国24个资源枯竭型城市旅游业效率,从静态和动态两个方面分析了旅游业效率情况。研究发现:我国资源枯竭型城市旅游业效率整体水平偏低,且城市间发展水平参差不齐,既存在明显的城市间差距,又呈现出较大的区域差异;根据旅游效率大小和变化情况两个维度可将资源枯竭型城市旅游业效率分为强力型、潜力型、乏力型和稳力型共四种类型。该结论为资源枯竭型城市科学发展旅游业,促进城市经济转型提供了新的思路和视角。

关键词：资源枯竭型城市旅游业效率 DEA mi

Roles of the canonical myomiRs mi R-1,-133 and-206 in cell development and disease

在线全文

学校读者我要写书评

暂无评论

World Journal of Biological Chemistry 2015年第3期6卷 162-208页

作者： Keith Richard mitchelson Wen-Yan Qin National Engineering Research Centre for Beijing Biochip Technology Medical Systems Biology Research Centre Tsinghua University School of Medicine

micro RNAs are small non-coding RNAs that participate in different biological processes, providing subtle combinational regulation of cellular pathways, often by regulating components of signalling pathways. Aberrant expression of mi RNAs is an important factor in the development and progression of disease. The canonical myomi Rs(mi R-1,-133 and-206) are central to the development and health of mammalian skeletal and cardiac muscles, but new findings show they have regulatory roles in the development of other mammalian non-muscle tissues, including nerve, brain structures, adipose and some specialised immunological cells. Moreover, the deregulation of myomi R expression is associated with a variety of different cancers, where typically they have tumor suppressor functions, although examples of an oncogenic role illustrate their diverse function in different cell environments. This review examines the involvement of the related myomi Rs at the crossroads between cell development/tissue regeneration/tissue inflammation responses, and cancer development.

关键词： Muscle micro RNAs mi R-1 mi R-206 mi R133a mi R-133b Cell development Cancer

布依语否定词mi11(不)和fi33(未)的语义和语法功能

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

民族语文 2013年第5期 48-55页

作者：陈娥周国炎云南师范大学文学院中央民族大学少数民族语言文学学院

通过对布依语否定词mi11(不)和fi33(未)的语义特征和语法功能进行分析,发现mi11和fi33的语义和语法功能相对对立,部分交叉;mi11能与多种词类和结构组合,能与fi33组合的词类和结构有限;mi11和fi33的前置性语序具有类型学特征。

关键词：布依语经历体壮语壮傣语支经验体 fi mi 否定词语法功能

DE TIAM mi ESTAS ESPERANTISTO

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

世界 1996年第Z2期 22-23页

作者： Verkis V.Setala

En 1903, iufoje mi vojais de Bukareto alKonstantinpolo, kaj mi prenis sidlokon en larestoracia vagono. Apenaǚ mi sidiis, mividis ke iu eksterlandano penas komprenigi alkelnero kion li deziras. La kelnero uzas sian tu-tan lingvoscion: li parolas rumane, serbe,turke, bulgare kaj ruse, sed vane! La fremd-landano ne komprenas e unu vorton.

关键词： DE TIAM mi ESTAS ESPERANTISTO mi La

Tumor suppressor role of mi R-133a in gastric cancer by repressing IGF1R

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第10期21卷 2949-2958页

作者： Yu Gong Jun Ren Kun Liu Li-ming Tang Department of Gastrointestinal Surgery Nanjing Medical University Affiliated Changzhou No. 2 People’s Hospital

AIM:To investigate the function and mechanism of mi R-133a in gastric cancer(GC)and its relationship with clinicopathological characteristics of ***:A total of 105 GC patients who underwent surgical resection as primary treatment were selected for this ***-time quantitative reverse transcriptase polymerase chain(q RT-PCR)was used to examine the expression levels of mi R-133a in human GC and adjacent non-tumor tissues,as well as in GC cell lines(SGC-7901,BGC-823,MGC-803,and AGS)and a human gastric mucosal epithelial cell line(GES-1).The biological role of mi RNA(mi R)-133a was assessed in the GC cell lines using MTT,apoptosis,migration and invasion,and colony formation assays,and xenograft tumorigenesis.q RT-PCR and western blot analyses were used to evaluate the potential target gene expression of mi ***’s correlation was calculated to evaluate the correlation between mi R-133a and insulinlike growth factor 1 receptor(IGF1R)*** regulation of IGF1R by mi R-133a was verified using the luciferase reporter ***:In 80%of the 105 GC patients,the mean expression of mi R-133a was significantly downregulated in tumor tissues compared with adjacent normal tissues(1.215±0.1477 vs 3.093±0.4104,Pmi R-133a was significantly correlated with the degree of differentiation(P=0.01),local invasion(P=0.001)and TNM stage(P=0.02)in GC *** with a control construct,forced expression of mi R-133a in GC cell lines inhibited proliferation(0.4787±0.0219 vs 0.7050±0.0147,P=0.0013 in SGC-7901 cells;and 0.5448±0.0085vs 0.7270±0.0084,P=0.001 in MGC-803 cells);migration(0.6333±0.0233 vs 1.037±0.0584,P=0.003 in SGC-7901 cells;0.6126±0.0311 vs 1.024±0.0456,P=0.0017 in MGC-803 cells);and invasion(0.613±0.0399 vs 1.033±0.0278,P=0.0013 in SGC-7901 cells;0.7433±0.0221 vs 1.017±0.0311,P=0.002 in MGC-803 cells).It also induced apoptosis(18.19%±0.2483%vs 5.887%±0.3837%,P<0.0001 in SGC-7901 cells;22.69%±0.7846%vs9.347%±0.301

关键词： Gastric cancer mi R-133a Tumor suppressor Insulin-

Predictive value and impact analysis for the index of microcirculatory resistance in mi patients with elective percutaneous coronary intervention

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Medical Abstracts(Internal Medicine) 2016年第3期33卷 153-154页

作者：王世超 Dept Cardiol Qingdao East Campus HospQingdao Univ Med Coll

Objective To evaluate the predictive value and impact for the index of microcirculatory resistance(IMR)in myocardial infarction(mi)patients with elective percutaneous coronary intervention(PCI)for treating coronary ar... 详细信息

关键词： mi IMR LVEF CRP Predictive value and impact analysis for the index of microcirculatory resistance in mi patients with elective percutaneous coronary intervention PCI

Inhibition of SW620 human colon cancer cells by upregulating mi RNA-145

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第9期22卷 2771-2778页

作者： Chen Li Na Xu Yu-Qiang Li Yu Wang Zhi-Tu Zhu Biobank The First Affiliated Hospital of Liaoning Medical University Department of Radiology The First Affiliated Hospital of Liaoning Medical University Department of Oncology The First Affiliated Hospital of Liaoning Medical University

AIM: To investigate the targeted inhibition of proliferation and migration of SW620 human colon cancer cells by upregulating mi RNA-145(mi R-145).METHODS: Forty-five samples of colon cancer tissues and 45 normal control samples were obtained from the biological database of the First Affiliated Hospital of Liaoning Medical University. We performed quantitative analysis of mi R-145 and N-ras expression in tissues; reverse transcriptase polymerase chain reaction analysis of mi R-145 expression in SW620 colon cancer cells and normal colonic epithelial cells; construction of mi R-145 lentiviral vector and determination of mi R-145 expression in SW620 cells transduced with mi R-145 vector; analysis of the effect of mi R-145 overexpression on SW620 cell proliferation; analysis of the effect of mi R-145 overexpression on SW620 cell migration using a wound healing assay; and analysis of the effect ofmi R-145 on N-ras expression using Western blotting. RESULTS: mi R-145 expression was significantly downregulated in colon cancer tissues, with its expression in normal colonic tissues being 4-5-fold higher(two sample t test, P mi R-145 expression in SW620 cells was downregulated, which was significantly lower compared to that in colonic epithelial cells(two sample t test, P mi R-145 vector and control were successfully packaged; expression of mi R-145 in SW620 cells transduced with mi R-145 was 8.2-fold of that in control cells(two sample t test, P mi R-145-transduced SW620 cells was significantly decreased compared to control cells(two sample t test, P migration indexes and controls were(97.27% ± 9.25%) and(70.22% ± 6.53%), respectively(two sample t test, P mi R-145-tranduced SW620 cells was significantly lower than others(one-way analysis of variance, P mi R-145 is important i

关键词： mi RNA-145 Colorectal cancer Targeted therapy N-ras Lentiviral vector