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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Liaotian Peng Yuanmei Wen Zhimei Yuan Yang Zhou Jiawei Yang Department of Biochemistry Zunyi Medical University

Chiral sulfoxides are valuable organosulfur compounds and have been widely used as important intermediates in asymmetric synthesis and as bioactive ingredients in pharmaceutical *** biocatalysis is a new approach for green synthesis of chiral ***,it is difficult to obtain enzymes with high catalytic efficiency and high substrate *** this study,a methionine sulfoxide reductase a gene was cloned from a Pseudomonas monteilii strain(named pm MsrA) and recombinant expressed in *** results show that this enzyme can effectively accomplish the preparation of(R)-sulfoxides with approximately 50% yield and 94–99% enantiomeric excess through asymmetric reductive resolution of racemic *** the establishment of the enzyme regeneration system,the(R)-sulfoxides were obtained with high enantioselectivity under the substrate concentration up to 200 mm(approximately 32 g/L),representing a quite high substrate concentration in biocatalytic preparation of chiral ***,this enzyme showed fairly good activity and enantioselectivity towards a series of ortho-and para-substituted phenyl methyl *** high initial substrate concentration,catalytic rate,and operability,as well as wide substrate scope of our system implies a potential future for industrial *** addition,the discovery and application of the pm MsrA strongly supports that asymmetric reductive resolution of rac-sulfoxides could be an alternative strategy for green synthesis of optically pure sulfoxides.

关键词： methionine sulfoxide reductase a recombinant enzyme chiral sulfoxides biocatalysis

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S-methyl-L-cysteine Protects against Antimycin A-induced Mitochondrial Dysfunction in Neural Cells via Mimicking Endogenous methionine-centered Redox Cycle

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Current Medical Science 2020年第3期40卷 422-433页

作者： Lan NI Xin-lei GUAN Fu-feng CHEN Peng-fei WU Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pharmacy Wuhan Puai HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430033China Tongji-Rongcheng Center for Biomedicine Wuhan 430030China Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province Wuhan 430030China

Mitochondrial superoxide overproduction is believed to be responsible for the neurotoxicity associated with ***-targeted antioxidants,such as MitoQ,have emerged as potentially effective antioxidant *** sulfoxide reductase A(MsrA)is a key mitochondrial-localized endogenous antioxidative enzyme and it can scavenge oxidizing species by catalyzing the methionine(Met)-centered redox cycle(MCRC).In this study,we observed that the natural L-Met acted as a good scavenger for antimycin A-induced mitochondrial superoxide overproduction in PC12 *** antioxidation was largely dependent on the Met oxidase activity of MsrA.S-methyl-L-cysteine(SMLC),a natural analogue of Met that is abundantly found in garlic and cabbage,could activate the Met oxidase activity of MsrA to scavenge free ***,SMLC protected against antimycin A-induced mitochondrial membrane depolarization and alleviated 1-methyl-4-phenylpyridinium(MPP+)-induced ***,our data highlighted the possibility for SMLC supplement in the detoxication of mitochondrial damage by activating the Met oxidase activity of MsrA.

关键词： methionine sulfoxide reductase a Met oxidase S-methyl-L-cysteine neurotoxin 1-methyl-4-phenylpyridinium

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