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Relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma

Hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 574-576页

作者： En-Hua Xiao Guo-Dong Hu Jin-Qing Li the Department of Radiology, Second Xiangya Hospital, Central South University, Changsha 410011, China Department of Radiology, Tongji Hospital, Tongji Medical University, Wuhan 430030, China and Hepatobiliary Department, Cancer Center of Sun Yat-Sen University of Medical Sciences, Guangzhou 510060, China Department of Radiology Second Xiangya Hospital Central South University Changsha 410011 China. xiaogdk@***

Objective: To study relationship between apoptosis and invasive and metastatic potential of hepatocellu- lar carcinoma (HCC). Methods: Apoptotic rate (AR), proliferative index (PI) and S-phase fraction (SPF) were measured by flow cytometry, and p170, p21 and nucleoside diphosphate kinase (ndpk) by strept avidin-biotin complex immunohistochemical technique in 57 pa- tients with HCC. Results: In this group, AR was 1.77% ±0.19%, SPF 12.55% ±0.68%, and PI 20.91% ±1.12% (r =-0.173). p170, p21 and ndpk positive rates were 61.36%, 68, 18%, 52.27% respectively in patients with a mean AR of ≤1.77%, and 23.08%, 38.46%, 84.62% respectively in patients with a mean AR of >1.77% (all Pmetastatic potential or other factors as proliferative activity, p21, p170 and ndpk.

关键词： hepatocellular carcinoma apoptosis metastatic potential flow cytometry immunohistochemistry

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HER2-induced metastasis is mediated by AKT/JNK/EMT signaling pathway in gastric cancer

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World Journal of Gastroenterology 2016年第41期22卷 9141-9153页

作者： Yiseul Choi Young San Ko Jinju Park Youngsun Choi Younghoon Kim Jung-Soo Pyo Bo Gun Jang Douk Ho Hwang Woo Ho Kim Byung Lan Lee Department of Tumor Biology Cancer Research Institute Seoul National Universit College of Medicine Department of Anatomy Seoul National University College of Medicine Department of Pathology Seoul National University College of Medicine Department of Pathology Eulji University Hospital Eulji University College of Medicine Department of Pathology Jeju National University Hospital Ischemic/Hypoxic Disease Institute Medical Research Center Seoul National University College of Medicine

AIM To investigated the relationships between HER2, c-Jun N-terminal kinase(JNK) and protein kinase B(AKT) with respect to metastatic potential of HER2-positive gastric cancer(GC) *** Immunohistochemistry was performed on tissue array slides containing 423 human GC specimens. Using HER2-positve GC cell lines SNU-216 and NCI-N87, HER2 expression was silenced by RNA interference, and the activations of JNK and AKT were suppressed by SP600125 and LY294002, respectively. Transwell assay, Western blot, semi-quantitative reverse transcriptionpolymerase chain reaction and immunofluorescence staining were used in cell culture experiments. RESULTS In GC specimens, HER2, JNK, and AKT activations were positively correlated with each other. In vitro analysis revealed a positive regulatory feedback loop between HER2 and JNK in GC cell lines and the role of JNK as a downstream effector of AKT in the HER2/AKT signaling pathway. JNK inhibition suppressed migratory capacity through reversing EMT and dual inhibition of JNK and AKT induced a more profound effect on cancer cell *** HER2, JNK and AKT in human GC specimens are positively associated with each other. JNK and AKT, downstream effectors of HER2, co-operatively contribute to the metastatic potential of HER2-positive GC cells. Thus, targeting of these two molecules in combination with HER2 downregulation may be a good approach to combat HER2-positive GC.

关键词： Gastric cancer HER2 c-Jun N-terminal kinase Protein kinase B metastatic potential

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