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An eukaryotic translation initiation factor, AteIF_5A-2,affects cadmium accumulation and sensitivity in Arabidopsis

Journal of Integrative Plant Biology 2015年第10期57卷 848-858页

作者： Xiao-Yan Xu Zhong-Jie Ding Lei Chen Jin-Ying Yan Gui-Xin Li Shao-Jian Zheng State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University College of Agronomy and Biotechnology Zhejiang University

Cadmium (Cd) is one of the most toxic elements and can be accumulated in plants easily; meanwhile, eIF5A is a highly conserved protein in all eukaryotic organisms. The present work tried to investigate whether eIF5A is involved in Cd accumulation and sensitivity in Arabidopsis (Arabidopsis thaliana L.) by comparing the wild-type Columbia-0 (Col-0) with a knockdown mutant of AteIF5A-2, fbr12-3 under Cd stress conditions. The results showed that the mutant fbr12-3 accumulated more Cd in roots and shoots and had significantly lower chlorophyll content, shorter root length, and smaller biomass, suggesting that downregulation of AteIF5A-2 makes the mutant more Cd sensitive. Real-time polymerase chain reaction revealed that the expressions of metal transporters involved in Cd uptake and translocation including IRT1, ZIP1, AtNramp3, and AtHMA4 were significantly increased but the expressions of PCS1 and PCS2 related to Cd detoxification were decreased notably in fbr12-3 compared with Col-0. As a result, an increase in MDA and H2O2 content but decrease in root trolox, glutathione and proline content under Cd stress was observed, indicating that a severer oxidative stress occurs in the mutant. All these results demonstrated for the first time that AteIF5A influences Cd sensitivity by affecting Cd uptake, accumulation, and detoxification in Arabidopsis.

关键词： Arabidopsis AteIF5A cadmium toxicity metal transporter oxidative stress

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HvFP3, a HMA domain protein interacted with the E3 ubiquitin ligase HvCMPG, is involved in wheat powdery mildew defense

HvFP3, a HMA domain protein interacted with the E3 ubiquitin...

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江苏省遗传学会2013年学术研讨会

作者： LI Yingbo WANG Wei FEI Fei CAO Aizhong WANG Haiyan XING Liping WANG Zongkuan CHENG Jiangyue ZHU Yanfei XIE Qi WANG Xiue National Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Powdery mildew is one of the most serious diseases threatened wheat production for last few decades. Haynaldia villosa L., species from wheat family, shows high powdery mildew resistance and confers the resistance gene Pm21. In our previous study, by using the Barley GeneChip microarray and cDNA library screening, a HvCMPG gene encoding E3 ligase has been cloned and was found to play an important role in the defense response to the powdery mildew disease. For further elucidating the molecular mechanism, a yeast two-hybrid library of H. villosa was constructed and used to screen the interact proteins with HvCMPG. A metal transporter gene was found to be interacted with the HvCMPG, and the full length was cloned from H. villosa and was designated as HvFP3. HvFP3 can interact with and be ubiquitinated by HvCMPG in vitro. Analysis by quantitative RT-PCR showed that HvFP3 was rapidly up-regulated in the leaf of *** after Blumeria graminis ***（Bgt） inoculation. The subcellular localization of the HvFP3 was in the nucleus and plasma membrane of the epidermal cell of onion. In the transient overexpression assay, it was found that the transient over-expression of HvFP3 led to significantly decreased in the haustoria index（HI） compared with the vector control in the epidermal cell of wheat variety Yangmai158, which is moderatly susceptible to powdery mildew disease. The HvFP3 transgenic plants over-expressed in Yangmai158 and the positive transgenic plants showed improved powdery mildew resistance than the receptor. These results indicated that the HvFP3 participated in the powdery mildew defense responses.

关键词： wheat powdery mildew yeast two hybrid metal transporter

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