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Asian Journal of Andrology 2006年第3期8卷 307-315,387页

作者： Ke-Hung Tsui Phei-Lang Chang Horng-Heng Juang Department of Urology Chang Gung Molecular Image CenterChang Gung Memorial Hospital Department of Urology Chang Gung Memorial Hospital Chang Gung Molecular Image Center Chang Gung Memorial Hospital Department of AnatomyChang Gung UniversityKwei-Shan.Tao-Yuan 333TaiwanChina

Aim:To investigate the possible role of manganese in the regulation of mitochondrial aconitase(mACON)activity human prostate carcinoma cell line PC-3 ***:The mACON enzymatic activities of human prostate carcinoma cell line PC-3 cells were determined using a reduced nicotinamide adenine dmucleotide-coupled assay. Immunoblot and transient gene expression assays were used to study gene expression of the *** putative response element for gene expression was identified using reporter assays with site-directed mutagenesis and electro- phoretic mobility-shift ***:In vitro study revealed that manganese chloride(MnCl2)treatment for 16h inhibited the enzymatic activity of mACON,which induced the inhibition of citrate utility and cell proliferation of PC- 3 *** results from transient gene expression assays showed that MnCl_2,treatment upregulated gene translation by approximately 5-fold through the iron response element pathway,immunoblot and reporter assays showed that MnCl_2 treatments inhibited protein and gene expression of *** effect was reversed by co- treatment with fenic ammonium *** reporter assays with site-directed mutagenesis and electrophoretic mobility-shift assays suggested that a putative metal response element in the promoter of the mACON gene was involved in the regulation of MnCl_2 on the gene expression of ***:These findings suggest that manganese acts as an antagonist of iron,disrupting the enzymatic activity and gene expression of mACON and citrate metabolism in the prostate.

关键词： citrate adenosine triphosphate proliferation PC-3 metal response element prostate carcinoma cell line

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