检索结果-南通市图书馆

mesenchymal stem cells as a potent cell source for articular cartilage regeneration

World Journal of Stem Cells 2014年第3期6卷 344-354页

作者： Mohamadreza Baghaban Eslaminejad Elham Malakooty Poor Department of Stem Cells Developmental Biology atCell Science Research Center Royan Institute for Stem Cell Bi-ology and Technology ACECR

Since articular cartilage possesses only a weak capac-ity for repair, its regeneration potential is considered one of the most important challenges for orthopedic surgeons. The treatment options, such as marrow stimulation techniques, fail to induce a repair tissue with the same functional and mechanical properties of native hyaline cartilage. Osteochondral transplantation is considered an effective treatment option but is as-sociated with some disadvantages, including donor-site morbidity, tissue supply limitation, unsuitable mechani-cal properties and thickness of the obtained tissue. Although autologous chondrocyte implantation results in reasonable repair, it requires a two-step surgical pro-cedure. Moreover, chondrocytes expanded in culture gradually undergo dedifferentiation, so lose morpho-logical features and specialized functions. In the search for alternative cells, scientists have found mesenchymal stem cells(MSCs) to be an appropriate cellular mate-rial for articular cartilage repair. These cells were origi-nally isolated from bone marrow samples and further investigations have revealed the presence of the cells in many other tissues. Furthermore, chondrogenic dif-ferentiation is an inherent property of MSCs noticedat the time of the cell discovery. MSCs are known to exhibit homing potential to the damaged site at which they differentiate into the tissue cells or secrete a wide spectrum of bioactive factors with regenerative proper-ties. Moreover, these cells possess a considerable im-munomodulatory potential that make them the general donor for therapeutic applications. All of these topics will be discussed in this review.

关键词： mesenchymal stem cells Regeneration Articular cartilage Cell therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

mesenchymal Stromal Cells Derived from Human Embryonic Stem Cells, Fetal Limb and Bone Marrow Share a Common Phenotype but Are Transcriptionally and Biologically Different

引用

Stem Cell Discovery 2017年第1期7卷 1-26页

作者： Candida Vaz Betty Tan Bee Tee Delicia Yong Qian Yi Lee Vivek Tanavde Bioinformatics Institute A*STAR (Agency for Science Technology and Research) Singapore Department of Medical Microbiology Faculty of Medicine University of Malaya Kuala Lumpur Malaysia Institute of Medical Biology A*STAR (Agency for Science Technology and Research) Singapore

mesenchymal stromal cells (MSCs) can be obtained from several sources and the significant differences in their properties make it crucial to investigate the differentiation potential of MSCs from different sources to determine the optimal source of MSCs. We investigated if this biological heterogeneity in MSCs from different sources results in different mechanisms for their differentiation. In this study, we compared the gene expression patterns of phenotypically defined MSCs derived from three ontogenically different sources: Embryonic stem cells (hES-MSCs), Fetal limb (Flb-MSCs) and Bone Marrow (BM-MSCs). Differentially expressed genes between differentiated cells and undifferentiated controls were compared across the three MSC sources. We found minimal overlap (5% - 16%) in differentially expressed gene sets among the three sources. Flb-MSCs were similar to BM-MSCs based on differential gene expression patterns. Pathway analysis of the differentially expressed genes using Ingenuity Pathway Analysis (IPA) revealed a large variation in the canonical pathways leading to MSC differentiation. The similar canonical pathways among the three sources were lineage specific. The Flb-MSCs showed maximum overlap of canonical pathways with the BM-MSCs, indicating that the Flb-MSCs are an intermediate source between the less specialised hES-MSC source and the more specialised BM-MSC source. The source specific pathways prove that MSCs from the three ontogenically different sources use different biological pathways to obtain similar differentiation outcomes. Thus our study advocates the understanding of biological pathways to obtain optimal sources of MSCs for various clinical applications.

关键词： mesenchymal Stromal Cells (MSCs) Human Embryonic Stem Cells Derived MSCs (hES-MSCs) Fetal Limb Derived MSCs (Flb-MSCs) Bone Marrow Derived MSCs (BM-MSCs) Ontogenically Different Sources Source Specific Canonical Pathways

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fluid Shear Stress-Induced IL-8/CXCR Signaling Promotes Epithelial mesenchymal Transition of Ovarian Cancer Cells

引用

医用生物力学 2019年第A1期34卷 140-141页

作者： Jirui Wen Jiang Wu Deep Underground Space Medical Center West China HospitalSichuan University

Objective Epithelial mesenchymal transition(EMT)plays a very important role in ovarian cancer metastasis,and IL-8 released from mechanosensitive cancer cells may contribute to the EMT process of solid carcinomas.In this study,we have explored IL-8 and its receptors signal transduction process of human ovarian cancer cells under conditions of FSS,and simultaneously detected the EMT process of ovarian cancer.Methods After the fluid shear stress was loaded,LightCyclerTM system and ELISA were employed to assay the IL-8 mRNA expression and protein production,respectively.Meanwhile,IL-8 reporter gene pEGFP1-IL8USCS was constructed for determining IL-8 gene transcriptional activation through gene transfer and flow cytometric analysis.RT-PCR,Northern blot and immunofluorescence were used to determine the expression of IL-8 receptor CXCR2 at mRNA and protein levels.IL-8 downstream signaling molecule NF-κB nuclear translocation was observed by immunocytofluoresent staining.Western blot was used to examine IκB phosphorylation and EMT-related protein.Results(1)The increase of IL-8 mRNA expression by shear stress was time-dependent.The expression increased when SKOV3 cells exposed to fluid shear stress for 1 h,reached the summits at 2 h,gradually decreased at 3 h and remained at a constant level at 4~12 h.Additionally,IL-8 expression was negatively associated with the intensity of shear stress.After SKOV3 cells were exposed to low fluid shear stress(1.5 dyne/cm2)for 1 h and 2 h,IL-8 mRNA expression increased near 68 and 52 times respectively as that of SKOV3 cells exposed to a high fluid shear stress of 5.0 dyne/cm^2.(2)The productions of IL-8 protein in SKOV3 cells subjected to shear stress were time-dependent.The secretion reached the summit when SKOV3 cells exposed to fluid shear stress for 5 h,then IL-8 secretion gradually decreased at 8 h of stimulation by shear stress.IL-8 secretion increased obviously when fluid shear stress(0.5,1.5,or 2.0 dyne/cm2)was exerted on SKOV3 cells for 1 h.Notablely,the secretion of IL-8 was

关键词： ovarian cancer interleukin-8 NF-κB p65 fluid shear stress epithelial mesenchymal transition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three kinds of mesenchymal stem cells:differentiating into neuron-like cells

引用

中国组织工程研究 2018年第33期22卷 5356-5360页

作者： Yang Ying Xu Qian Wang Hui-ling Yao Min-xiu Department of Endocrinology Second Affiliated Hospital of Qingdao University Medical SchoolQingdao 266042Shandong ProvinceChina

BACKGROUND:Stem cells,characterized with convenient collection,wide sources and great potential,have been developed in the cell therapy.The most commonly used stem cells include bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells.OBJECTIVE:To compare the biological changes in bone marrow mesenchymal stem cells,placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells after co-cultured with nerve cells.METHODS:Human bone marrow mesenchymal stem cells,human placental mesenchymal stem cells and human umbilical cord blood mesenchymal stem cells were isolated and cultured in vitro.The morphological changes of mesenchymal stem cells differentiated into nerve cells were observed by co-culture with Transwell culture plates.The expression of neuron-specific enolase was detected after induction.RESULTS AND CONCLUSION:In the co-culture system,these three kinds of mesenchymal stem cells gradually formed a star-like shape and were interconnected.Moreover,all the cells were positive for neuron-specific enolase.These findings reveal that the microenvironment provided by nerve cells can induce and promote the differentiation of three kinds of mesenchymal stem cells into neuron-like cells.

关键词： mesenchymal Stem Cells Coculture Techniques Tissue Engineering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

mesenchymal stem cells in the treatment of inflammatory and autoimmune diseases in experimental animal models

引用

World Journal of Stem Cells 2015年第3期7卷 556-567页

作者： Matthew W Klinker Cheng-Hong Wei Gene Transfer and Immunogenicity Branch Division of Cellular and Gene TherapiesOffice of Cellular Tissue and Gene Therapies Food and Drug Administration Center for Biologics Evaluation and ResearchSilver Spring MD 20993-0002 United States

Multipotent mesenchymal stromal cells [also known as mesenchymal stem cells(MSCs)] are currently being studied as a cell-based treatment for inflammatory disorders. Experimental animal models of human immune-mediated diseases have been instrumental in establishing their immunosuppressive properties. In this review, we summarize recent studies examining the effectiveness of MSCs as immunotherapy in several widely-studied animal models, including type 1 diabetes, experimental autoimmune arthritis, experimental autoimmune encephalomyelitis, inflammatory bowel disease, graft-vs-host disease, and systemic lupus erythematosus. In addition, we discuss mechanisms identified by which MSCs mediate immune suppression in specific disease models, and potential sources of functional variability of MSCs between studies.

关键词： mesenchymal stromal cells mesenchymalstem cells Autoimmunity Animal models Inflammation Immunotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plexiform fibromyxoma:Review of rare mesenchymal gastric neoplasm and its differential diagnosis

引用

World Journal of Gastrointestinal Oncology 2021年第5期13卷 409-423页

作者： Mustafa Erdem Arslan Hua Li Zhiyan Fu Timothy A Jennings Hwajeong Lee Pathology and Laboratory Medicine Albany Medical CenterAlbanyNY 12208United States

Plexiform fibromyxoma(PF)is a very rare mesenchymal neoplasm of the stomach that was first described in 2007 and was officially recognized as a subtype of gastric mesenchymal neoplasm by World Health Organization(WHO)in 2010.Histologically,PF is characterized by a plexiform growth of bland spindle to ovoid cells embedded in a myxoid stroma that is rich in small vessels.The lesion is usually paucicellular.While mucosal and vascular invasion have been documented,no metastasis or malignant transformation has been reported.Its pathogenesis is largely unknown and defining molecular alterations are not currently available.There are other mesenchymal tumors arising in the gastrointestinal tract that need to be differentiated from PF given their differing biologic behaviors and malignant potential.Histologic mimics with spindle cells include gastrointestinal stromal tumor,smooth muscle tumor,and nerve sheath tumor.Histologic mimics with myxoid stroma include myxoma and aggressive angiomyxoma.Molecular alterations that have been described in a subset of PF may be seen in gastroblastoma and malignant epithelioid tumor with gliomaassociated oncogene homologue 1(GLI1)rearrangement.The recent increase in publications on PF reflects growing recognition of this entity with expansion of clinical and pathologic findings in these cases.Herein we provide a review of PF in comparison to other mesenchymal tumors with histologic and molecular resemblance to raise the awareness of this enigmatic neoplasm.Also,we highlight the challenges pathologists face when the sample is small,or such rare entity is encountered intraoperatively.

关键词： Plexiform Fibromyxoma Gastrointestinal mesenchymal Neoplasm Stomach

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Germline cells derived from mesenchymal stem cells, with the focus on Wharton's jelly

引用

Asian pacific Journal of Reproduction 2018年第2期7卷 49-55页

作者： Hossein Yazdekhasti Jalil Hosseini Zahra Rajabi Maryam Hosseinzadeh Shirzeyli Fereshte Aliakbari Department of Cell Biology Center for Research in Contraceptive and Reproductive HealthUniversity of VirginiaCharlottesvilleVA22908USA Infertility and Reproductive Health Research Center Shahid Beheshti University of Medical SciencesTehranIran Department of Anatomy School of MedicineTehran University of Medical SciencesTehranIran Department of Biomedical Engineering University of VirginiaCharlottesvilleVA22908USA Department of Biology and Anatomical Sciences School of MedicineShahid Beheshti University of Medical SciencesTehranIran

Previous attempts have indicated that mesenchymal stem cells (MSCs) are a valuable source and candidate and new approach for tissue engineering and reproductive medicine. MSCs have this potential to be induced and differentiated in an appropriatein vivoandin vitro condition toward various cell lineages and then they can be applied in cell therapies and clinical applications. During recent two decades, various sources have demonstrated they are a great source for MSCs, including bone marrow, the human umbilical cord as well as Wharton's jelly. Due to discarding after birth, easily accessible cells and less ethical concerns, these cells have attracted more and more scientists' attention. Infertility and reproduction diseases have provided special opportunity to examine the efficiency of MSCs in this kind of application. Based on recent investigations, MSCs embedded in Wharton's jelly tissue are more appealing for cell therapies, especially in infertility treatment purposes. So, differentiation of MSCs embedded in Wharton's jelly tissue into germ layer cells for cell-based therapy purposes is now under intensive study.

关键词： mesenchymal stem cell Bone marrow stem cell Umbilical cord Wharton's jelly

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Modulation Effects of the 3D Strain Microenvironment on Characteristics of mesenchymal Stem Cell-Derived Exosomes

引用

医用生物力学 2019年第A1期34卷 128-129页

作者： Yufei Ma Wenting Yu Feng Xu School of Life Science and Technology Xi’an Jiaotong UniversityXi’an710049China Stomatological Hospital College of MedicineXi’an Jiaotong UniversityXi’an710004China

It is necessary to investigate the characteristics of mesenchymal stem cells(MSCs)derived exosomes,and especially their application in tissue regeneration.Previous studies have shown that inflammatory stimulation enhanced the secretion of MSC-derived exosomes with stronger anti-inflammatory protein,cytokine profiles,and functional RNA via altering COX2/PGE2 pathway.Recently,accumulating evidence has also revealed that biophysical cues(especially biomechanical cues)in cell microenvironment have significant effects not only on cells but also on their exosomes.It has been reported that applying bi-axial strain to MSCs induces formation of a stiffer cytoskeleton through mTORC2 signaling,which biases against adipogenic differentiation and toward osteoblastogenesis.At the same time,For example,dimensionality,composition and stiffness of the extracellular matrix(ECM)has been proved to affect the size and composition of exosomes secreted by cancer cells.However,the effects of biomechanical cues in the three-dimensional(3D)microenvironment on stem cell-derived exosomes remains to be unveiled.Therefore,it is important to understand the roles of 3D cell mechanical microenvironment in regulating the characteristics of stem cell-derived exosomes and develop more efficient approaches to enhance their functions.This study aimed to explore the changes in characteristics of exosomes secreted by MSCs in periodontium in response to the matrix strain in 3D.Periodontal ligament stem cells(PDLSCs)were cultured in a 3D strain microenvironment engineered with microscale magnetically stretched collagen hydrogels.The morphology,particle distribution,marker protein expression of PDLSC-derived exosomes were analyzed.Then the pro-osteogenic property of exosomes was evaluated by assessing cell viability,proliferation,migration and osteogenic differentiation of target cells,for instance human bone marrow mesenchymal stem cells(hBMSCs).Detailed characterizations revealed that PDLSC-derived exosomes in the 3D strain mi-croenvironment were with s

关键词： Modulation Effects the 3D Strain Microenvironment Characteristics mesenchymal Stem Cell-Derived Exosomes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats

引用

Asian Pacific Journal of Tropical Biomedicine 2013年第3期3卷 174-181页

作者： Saber Sakr Laila Rashed Waheba Zarouk Rania El-Shamy Department of Zoology Faculty of ScienceMenoufia University Department of Biochemistry Faculty of MedicineCairo University Center of Human Genetics and Human Genome Research National Research Center

Objective:To evaluate the effect of mesenchymal stem cells(MSCs)in rats with anti-Thy 1,1nephritis.Methods:Female albino rats were divided into three groups,control group,anti-Thy 1,1group and treatment with i.v.MSCs group.MSCs were derived from bone marrow of male albino rats,Y—chromosome gene was detected by polymerase chain reaction in the kidney.Serum urea and creatinine were estimated for all groups.Kidney of all studied groups was examined histologically and histochemically(total carbohydrates and total proteins).DNA fragmentation and expression ofα-SMA were delected.Results:Kidney of animals injected with anti-Thy 1,1showed inflammatory leucocytic infiltration,hypertrophied glomeruli,tubular necrosis and congestion in the renal blood vessels.The kidney tissue also showed reduction of carbohydrates and total proteins together with increase in apoptosis and in expression ofα-SMA.Moreover,the levels of urea and creatinine were elevated.Treating animals with MSCs revealed that kidney tissue displayed an improvement in the histological and histochemical changes.Apoptosis andα-SMA expression were decreased,and the levels of urea and creatinine decreased.Conclusions:The obtained results demonstrated the potential of MSCs to ameliorate the structure and function of the kidney in rats with anti-Thy 1,1 nephritis possibly through the release of paracrine growth factor(s).

关键词： Anti-Thy1,1 Nephritis mesenchymal stem cells α-SMA Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

mesenchymal stem cells for kidney transplantation

引用

World Journal of Clinical Urology 2014年第2期3卷 87-95页

作者： Bron Lett Kisha N Sivanathan P Toby Coates Central Northern Adelaide Renal and Transplantation Service University of AdelaideRoyal Adelaide HospitalMawson LakesAdelaide 5000South AustraliaAustralia

The long term consequence of immunosuppressive therapy in kidney transplantation has prompted investigation of alternative means to modify the immune response to the allograft. Cell based therapies are potentially attractive as they may provide a long lasting immunomodulatory effect, may repair tissues and reduce the necessity to take immunosuppressive drug therapy. Of the current cell therapies, mesenchymal stem cells have now been trialled in small numbers of human kidney transplantation with apparent safety and potential efficacy. Many issues however need to be resolved before these cells will become mainstays of transplant immunosuppression including ex vivo modification to enhance immunomodulatory properties, cell number, route and frequency of administration as well as cellular source of origin.

关键词： mesenchymal stem cells Kidney transplantation Immunosuppression Solid organ transplantation Cellular therapies

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：