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Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Frontiers of Environmental Science & Engineering 2020年第6期14卷 41-51页

作者： Lu Song Can Wang Yizhu Wang Indoor Air Environment Quality Control Laboratory of Tianjin School of Environment Science and EngineeringTianjin UniversityTianjin 300350China

Antibiotic resistance genes(ARGs)have been detected in various atmospheric *** ARGs transmission presents the public health ***,it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of *** this study,an optimized protocol for collecting and detecting airborne ARGs was *** results showed that recovery efficiency tended to increase initially and then declined over time,and a range of 550-780 copies/mmz of capture loading was recommended to ensure that the recovery efficiency is greater than 75%.As the cell walls were mechanically disrupted and nucleic acids were released,the buffer wash protects ARGs *** ratios of buffer volume to membrane area in buffer wash were *** highest concentrations of airborne ARGs were detected with 1.4μL/mm^2 buffer ***,the majority of the cells were disrupted by an ultrasonication pretreatment(5 min),allowing the efficiency ARGs detection of airborne ***,extending the ultrasonication can disrupt cell structures and gene sequence was broken down into ***,this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different *** optimized sampling method was proposed that the buffer wash was 1.4 nL/mm and the ultrasonication duration was 5 *** indoor airborne ARGs were examined in accordance with the improved protocol in two *** result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

关键词： Airborne tetracycline resistance genes Filter sampling Capture loading membrane pretreatment Indoor laboratory atmosphere

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Surface modification techniques of membranes to improve their antifouling characteristics:recent advancements and developments

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Frontiers of Chemical Science and Engineering 2023年第12期17卷 1837-1865页

作者： Muhammad Tawalbeh Haya Aljaghoub Muhammad Qasim Amani Al-Othman Sustainable and Renewable Energy Engineering Department University of SharjahSharjah 27272United Arab Emirates Sustainable Energy&Power Systems Research Centre RISEUniversity of SharjahSharjah 27272United Arab Emirates Industrial Engineering and Engineering Management Department University of SharjahSharjah 27272United Arab Emirates Department of Chemical and Biological Engineering American University of SharjahSharjah 26666United Arab Emirates Materials Science and Engineering Program College of Arts and SciencesAmerican University of SharjahSharjah 26666United Arab Emirates

Extensive research efforts are currently devoted to developing and improving conventional technologies for water treatment. membrane-based water treatment technologies are among the most preferred options due to their commercial success, simple operation, low energy and space requirements, and high separation efficiency. Despite the advances made in membrane-based technologies, fouling remains a critical challenge. Fouling occurs upon the accumulation of unwanted impurities on the membrane surface and within the membrane pores which results in a significant decline in the membrane permeate flux. To alleviate the operational challenges from fouling, surface modification to develop antifouling membranes appears to be an effective technique. A comprehensive review of the surface modification techniques for the development of antifouling membranes is provided in this paper. Chemical surface modification techniques (grafting and plasma treatment), physical modification techniques (blending, coating, adsorption, and thermal treatment), and combined physical and chemical modification techniques have been discussed. Moreover, the challenges related to surface modification and the future research directions are addressed.

关键词： fouling antifouling membrane surface modification membrane pretreatment

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