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Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves

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Journal of Integrative plant Biology 2007年第9期49卷 1327-1333页

作者： Yanmei Li Yantao Jia Zhongkai Zhang Xiaoying Chen Hongping He Rongxiang Fang Xiaojiang Hao State Key Laboratory of Photochemistry and plant Resources in West China Kunming Institute of Botany the Chinese Academy of Sciences Kunming 650204 China State Key Laboratory of plant Genomics Institute of Microbiology the Chinese Academy of Sciences Beijing 100080 China Laboratory of plant Virology Yunnan Academy of Agricultural Sciences Kunming 650223 China The Graduate School of the Chinese Academy of Sciences Beijing 100049 China

A new ribosome-inactivating protein （RIP） with a molecular weight of 31 kDa induced by Cinchonaglycoside C （1） designated CIP31, was isolated from tobacco leaves. Analysis of this protein sequence indicated that it belongs to the RIP family and it was distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence. CIP31 can directly impair synthesis of coat protein （CP） of tobacco mosaic virus （TMV）, which resulted in inhibition of TMV long distance movement and multiplication in tobacco plants at concentrations of ng/mL. Furthermore, no toxicity was shown to the growth and fertility of the plants. CIP31 was synthesized only in the presence of Cinchonaglycoside C （1） and was independent of the salicylic acid （SA） signal pathway. We provided evidence for the SA-independent biological induction of resistance.

关键词： antiviral protein Cinchonaglycoside C (1) coat protein medical plant ribosome inactivating protein salicylic acid tobacco mosaic virus.

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Moisture-dependent physical and mechanical properties of cumin(Cuminum cyminum L.)seed

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International Journal of Agricultural and Biological Engineering 2009年第2期2卷 49-56页

作者： Kaveh Mollazade Hojat Ahmadi Jalal Khorshidi Ali Rajabipour Seyed Saeid Mohtasebi Department of Agricultural Machinery Engineering Faculty of Biosystems EngineeringUniversity of TehranP.O.Box 4111Karaj 31587-77871Iran Department of Horticulture Science Faculty of Horticulture and Plant ProtectionUniversity of TehranKarajIran

Some physical and mechanical properties of cumin seed were obtained as moisture content changed from 7.24%to 21.38%*** of moisture content was found to increase the seed length(5.14-5.58 mm),width(1.33-1.55 mm),thickness(0.97-1.05 mm),arithmetic mean diameter(2.48-2.73),geometric mean diameter(1.88-2.09 mm),surface area(10.34-12.66 mm^(2)),thousand seed weight(2.9-3.9 g),porosity(51.22%-64.11%),true density(917.8-1030.6 kg/m3),static angle of repose(43-49 deg),dynamic angle of repose(47-56.6 deg),and coefficient of static friction on the three surfaces:glass(0.48-0.77),galvanized iron sheet(0.36-0.73),and plywood(0.57-0.69).However,bulk density was found to decrease from 447.66-369.88 kg/m3,and rupture force and rupture energy along with seed length and width were found to decrease from 83.74-56.17 N,132.95-84.47 N,50.66-27.52 mJ,and 67.8 to 33.36 mJ,*** sphericity increased from 36.63%to 37.5%with increasing in moisture content from 7.24%to 14.5%*** then reduced to 37.5%with further increase in moisture content to 21.38%d.b.

关键词： seed engineering properties moisture porosity density medical plant

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新疆20种药用植物的染色体观察

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西北植物学报 1985年第2期 149-154+173-174页

作者：马兴华覃若林邢文斌新疆医学院药学系

本文对在新疆生长和引种栽培的10科20种药用植物染色体进行了计数和研究,其中5种进行了核型分析,6种为首次报道。

关键词： medical plant Chromosome Karyotype

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