检索结果-南通市图书馆

Crude extract of maggots:Antibacterial effects against Escherichia coli,underlying mechanisms,separation and purification

引用

World Journal of Gastroenterology 2015年第5期21卷 1510-1517页

作者： Quan-Sheng Ge Hui-Min Zhang Xia Liu Shou-Yu Wang De-Cheng Lv Xu-Dong Li the First Affiliated Hospital of Dalian Medical University

AIM:To investigate the antibacterial effects of a crude extract of maggots against Escherichia coli(***) and the underlying mechanisms,and to separate and purifythe crude extract of maggots to assess the antibacterial effects of the active ingredients in the crude ***:Different concentrations of the crude extract of maggots were incubated with ***(O157:H7) and *** optical density(OD) was measured at different time points to plot the OD-T *** effects of different concentrations of the crude extract on bacterial membrane permeability were determined by fluorescence probe *** effects of different concentrations of the crude extract on plasmid DNA replication were determined by agarose gel ***-Sepharose ion exchange chromatography and Sephacryls-200 HR gel filtration chromatography were used to separate and purify the crude extract of *** molecular weight of proteins in the purified crude extract was determined by SDS-PAGD electrophoresis,and its antibacterial effects were determined by turbidimetric ***:The antibacterial effects of the crude extract of maggots at concentrations > 0.5 mg/m L were *** antibacterial effects of the crude extract at concentrations of 1.0,1.5 and 2.0 mg/m L did not differ *** probe analysis showed that the rate of membrane permeability change was 1223.1% in bacteria incubated with 2 mg/m L of the crude extract,and 1300.0% in those incubated with 80 mg/m L of the crude *** DNA was undetectable in *** incubated with 2 and 80 mg/m L of the crude extract.A low molecular weight protein band(about 15 k Da) was detected in the crude extract of maggots and eluent,but not in eluant,from DEAE-Sepharose ion exchange *** antibacterial effects of the crude extract of maggots and eluent were superior to those of eluant,with the antibacterial effects of eluents being better than those of the crude extract of maggot

关键词： Maggots Antibacterial peptide Antibacterial mechan

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ursodeoxycholic acid induces apoptosis in hepatocellular carcinoma xenografts in mice

引用

World Journal of Gastroenterology 2015年第36期21卷 10367-10374页

作者： Hui Liu Hong-Wei Xu Yu-Zhen Zhang Ya Huang Guo-Qing Han Tie-Jun Liang Li-Li Wei Cheng-Yong Qin Cheng-Kun Qin Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Department of Surgery Heze Peony District People’s Hospital Department of General Surgery Shandong Provincial Hospital Affiliated to Shandong University

AIM: To evaluate the efficacy of ursodeoxycholic acid(UDCA) as a chemotherapeutic agent for the treatment of hepatocellular carcinoma(HCC).METHODS: BALB/c nude mice were randomized into four groups 24 h before subcutaneous injection of hepatocarcinoma BEL7402 cells suspended in phosphate buffered saline(PBS) into the right flank. The control group(n = 10) was fed a standard diet while treatment groups(n = 10 each) were fed a standard daily diet supplemented with different concentrations of UDCA(30,50 and 70 mg/kg per day) for 21 d. Tumor growth was measured once each week,and tumor volume(V) was calculated with the following equation: V =(L × W2) × 0.52,where L is the length and W is the width of the xenograft. After 21 d,mice were killed under ether anesthesia,and tumors were excised and weighed. Apoptosis was evaluated through detection of DNA fragmentation with gel electrophoresis and the terminaldeoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling(TUNEL) assay. Western blot analysis was performed to determine the expression of apoptosisrelated proteins BAX,BCL2,APAF1,cleaved caspase-9,and cleaved ***: UDCA suppressed tumor growth relative to controls. The mean tumor volumes were the following: control,1090 ± 89 mm3; 30 mg/kg per day,612 ± 46 mm3; 50 mg/kg per day,563 ± 38 mm3; and 70 mg/kg per day,221 ± 26 mm3. Decreased tumor volumes reached statistical significance relative to control xenografts(30 mg/kg per day,P < 0.05; 50 mg/kg per day,P < 0.05; 70 mg/kg per day,P < 0.01). Increasing concentrations of UDCA led to increased DNA fragmentation observed on gel electrophoresis and in the TUNEL assay(control,1.6% ± 0.3%; 30 mg/kg per day,2.9% ± 0.5%; 50 mg/kg per day,3.15% ± 0.7%,and 70 mg/kg per day,4.86% ± 0.9%). Western blot analysis revealed increased expression of BAX,APAF1,cleaved-caspase-9 and cleaved-caspase-3 proteins,which induce apoptosis,but decreased expression of BCL2 protein,which is an inhibitor of apoptosis,following

关键词： Hepatocellular carcinoma Inhibitory effects mechan

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：