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Calibration curve and support vector regression methods applied for quantification of cement raw meal using laser-induced breakdown spectroscopy

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Plasma Science and Technology 2019年第3期21卷 23-30页

作者： Junwei JIA Hongbo FU Zongyu HOU Huadong WANG Zhibo NI Fengzhong DONG Anhui Provincial Key Laboratory of Photonic Devices and Materials Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences University of Science and Technology of China State Key Lab of Power Systems Department of Thermal Engineering Tsinghua-BP Clean Energy Center Tsinghua University

Laser-induced breakdown spectroscopy(LIBS) is a qualitative and quantitative analytical technique with great potential in the cement industrial analysis. Calibration curve(CC) and support vector regression(SVR) methods coupled with LIBS technology were applied for the quantification of three types of cement raw meal samples to compare their analytical concentration range and the ability to reduce matrix effects, respectively. To reduce the effects of fluctuations of the pulse-to-pulse, the unstable ablation and improve the reproducibility, all of the analysis line intensities were normalized on a per-detector basis. The prediction results of the elements of interest in the three types of samples, Ca, Si, Fe, Al, Mg, Na, K and Ti, were compared with the results of the wet chemical analysis. The average relative error(ARE),relative standard deviation(RSD) and root mean squared error of prediction(RMSEP) were employed to investigate and evaluate the prediction accuracy and stability of the two prediction methods. The maximum average ARE of the CC and SVR methods is 34.62% instead of 6.13%,RSD is 40.89% instead of 7.60% and RMSEP is 1.34% instead of 0.43%. The results show that SVR method can accurately analyze samples within a wider concentration range and reduce the matrix effects, and LIBS coupled with it for a rapid, stable and accurate quantification of different types of cement raw meal samples is promising.

关键词： cement raw meal calibration curves SVR LIBS

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Net energy of corn,soybean meal and rapeseed meal in growing pigs

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Journal of Animal Science and Biotechnology 2017年第4期8卷 875-884页

作者： Zhongchao Li Yakui Li Zhiqian Lv Hu Liu Jinbiao Zhao Jean Noblet Fenglai Wang Changhua Lai Defa Li State Key Laboratory of Animal Nutrition Ministry of Agriculture Feed Industry CentreChina Agricultural University INRA UMR Pegase

Background: Two experiments were conducted to estimate the net energy(NE) of corn, soybean meal, expel er-pressed rapeseed meal(EP-RSM) and solvent-extracted rapeseed meal(SE-RSM) using indirect calorimetry and to validate the NE of these four ingredients using pig growth ***: In Exp.1, 24 barrows(initial BW = 36.4 ± 1.6 kg) were allotted to 1 of 4 diets which included a corn basal diet,a corn-soybean meal basal diet and two rapeseed meal diets containing 20% EP-RSM(9.5% ether extract) or SE-RSM(1.1% ether extract) substituted for corn and soybean meal. The design allowed the calculation of NE values of corn,soybean meal and rapeseed meals according to the difference method. In Exp.2, 175 growing pigs(initial BW = 36.0± 5.2 kg) were fed 1 of 5 diets for 28 d, with five pigs per pen and seven replications(pens) per treatment in order to validate the measured energy values. Diets were a corn-soybean meal diet and four diets including 10% or 20% EP-RSM and 10% or 20% ***: The NE of corn, soybean meal, EP-RSM and SE-RSM were 12.46, 11.34, 11.71 and 8.83 MJ/kg DM, respectively. The NE to ME ratio of corn(78%) was similar to tabular values, however, the NE to ME ratios of soybean meal(70%) and rapeseed meal(76%) were greater than tabular values. The greater NE value in EP-RSM than in SE-RSM is consistent with its higher EE content. Increasing EP-RSM or SE-RSM did not affect the growth performance of pigs and the caloric efficiency of NE was comparable for al ***: The NE of EP-RSM was similar to soybean meal, and both were greater than SE-RSM. The DE, ME and NE values measured in Exp.1 are confirmed by results of Exp. 2 with comparable caloric efficiencies of DE, ME or NE for all diets.

关键词： Caloric efficiency Growing pig Heat production Net energy Rapeseed meal

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Partially defatted black soldier fly larva meal inclusion in piglet diets: effects on the growth performance, nutrient digestibility,blood profile, gut morphology and histological features

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Journal of Animal Science and Biotechnology 2019年第3期10卷 708-718页

作者： Ilaria Biasato Manuela Renna Francesco Gai Sihem Dabbou Marco Meneguz Giovanni Perona Silvia Martinez Ana Cristina Barroeta Lajusticia Stefania Bergagna Luca Sardi Maria Teresa Capucchio Enrico Bressan Andrea Dama Achille Schiavone Laura Gasco Department of Agricultural Forest and Food SciencesUniversity of TurinLargo P.Braccini 210095 Grugliasco(TO)Italy Department of Veterinary Sciences University of TurinLargo P.Braccini 210095 Grugliasco(TO) TurinItaly National Research Council Institute of Science of Food ProductionLargo P.Braccini 210095 Grugliasco(TO)Italy SDSV-Teaching Structure of Veterinary Medicine University of TurinLargo P.Braccini 210095 Grugliasco(TO)Italy Department of Animal Production University of MurciaCampus de Espinardo30071 MurciaSpain Department of Animal and Food Science Autonomous University of BarcelonaCerdanyola del Vallès08193 BellaterraBarcelonaSpain Veterinary Medical Research Institute for Piemonte Liguria and the Valle D’AostaVia Bologna 14810154 TurinItaly Department of Veterinary Medical Sciences University of BolognaVia Tolara di Sopra 5040064 Ozzano dell’Emilia(BO)Italy

Background:The aim of this trial was to investigate the effects of different inclusion levels of a partially defatted black soldier fly (BSF,Hermetia illucens L.) larva meal on the growth performance,nutrient digestibility,blood profile,gut morphology and histological features of piglets.A total of 48 newly weaned piglets were individually weighed (initial body weight (IBW):6.1 ± 0.16 kg) and randomly allocated to 3 dietary treatments (4 boxes as replicates/treatment and 4 animals/box).BSF larva meal was included at increasing levels (0% [BSF0],5% [BSFS] and 10% [BSF10]) in isonitrogenous and isoenergetic diets formulated for two feeding phases:Ⅰ (from d 1 to d 23) and Ⅱ (from d 24 to d 61).The weight gain (WG),average daily gain (ADG),average daily feed intake (ADFI) and feed conversion ratio (FCR) were calculated for each feeding phase and for the whole *** haematochemical parameters and nutrient digestibility of the piglets were also evaluated.A total of 3 piglets per box were slaughtered on d 61 and the slaughtered piglets were submitted to morphometric investigations and histopathological ***:No overall significant differences were observed for growth performance (P > 0.05),except for the ADFI of phase Ⅱ,which showed a linear response to increasing BSF meal levels (P meal inclusion did not significantly influence the blood profile,except as far as monocytes and neutrophils are concerned,and these showed a linear and quadratic response,respectively,to increasing BSF meal levels (P meal inclusion (P > 0.05).Conclusions:The obtained results show that a partially defatted BSF larva meal can be used as a feed ingredient in diets for weaned piglets without negatively affecting their growth performance,nutrient digestibilit

关键词： Hermetia il ucens L. Histopathology Insect meal Performance Weaning pigs

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Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets

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Journal of Animal Science and Biotechnology 2017年第4期8卷 957-969页

作者： M. Renna A. Schiavone F. Gai S. Dabbou C. Lussiana V. Malfatto M. Prearo M. T. Capucchio I. Biasato E. Biasibetti M. De Marco A. Brugiapaglia I. Zoccarato L. Gasco Department of Agricultural Forest and Food Sciences University of TorinoLargo P. Braccini 2 10095 Grugliasco TO Italy Department of Veterinary Sciences University of Torino Largo P. Braccini 2 10095 Grugliasco TO Italy Institute of Science of Food Production National Research Council Largo P.Braccini 2 10095 Grugliasco TO Italy Veterinary Medical Research Institute for Piedmont Liguria and Aosta Valley Via Bologna 148 10154 Torino Italy

Background: Two trials were performed to evaluate a partially defatted Hermetia illucens(HI) larvae meal as potential feed ingredient in rainbow trout(Oncorhynchus mykiss Walbaum) diets. In the first trial, 360 trout(178.9 ± 9.8 g of mean initial body weight) were randomly divided into three experimental groups(4 tanks/treatment, 30 fish/tank). The fish were fed for 78 days with isonitrogenous, isolipidic and isoenergetic diets containing increasing levels of HI, on as fed basis: 0%(HI0, control diet), 25%(HI25) and 50%(HI50) of fish meal substitution, corresponding to dietary inclusion levels of 0, 20% and 40%. In the second trial, 36 trout(4 tanks/treatment, 3 fish/tank) were used to evaluate the in vivo apparent digestibility coefficients(ADC) of the same diets used in the first ***: Survival, growth performance, condition factor, somatic indexes, and dorsal fillet physical quality parameters were not affected by diet. The highest dietary inclusion of HI larvae meal increased dry matter and ether extract contents of trout dorsal fillet. The use of HI larvae meal induced a decrease of valuable polyunsaturated fatty acids(PUFA) even if differences were only reported at the highest level of HI inclusion. The insect meal worsened the lipids health indexes of the same muscle. Dietary inclusion of insect meal did not alter the villus height of the fish. No differences were found among treatments in relation to ADC of ether extract and gross energy, while ADC of dry matter and crude protein were higher in HI25 if compared to ***: The obtained results showed that a partially defatted HI larvae meal can be used as feed ingredient in trout diets up to 40% of inclusion level without impacting survival, growth performance, condition factor, somatic indexes, dorsal fillet physical quality parameters, and intestinal morphology of the fish. However, further investigations on specific feeding strategies and diet formulations are needed to limit the observe

关键词： Animal performance Apparent digestibility coefficient Carnivorous fish Fatty acids Fishmeal substitution Insect meal

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Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens: apparent nutrient digestibility,apparent metabolizable energy and apparent ileal amino acid digestibility

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Journal of Animal Science and Biotechnology 2017年第4期8卷 897-905页

作者： Achille Schiavone Michele De Marco Silvia Martínez Sihem Dabbou Manuela Renna Josefa Madrid Fuensanta Hernandez Luca Rotolo Pierluca Costa Francesco Gai Laura Gasco Department of Veterinary Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Animal Production University of Murcia Campus de Espinardo 30071 Murcia Spain Department of Agricultural Forest and Food Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Institute of Science of Food Production National Research Council Largo Paolo Braccini 2 10095 Grugliasco TO Italy

Background: The study aimed to determine the apparent total tract digestibility coefficients(ATTDC) of nutrients, the apparent metabolizable energy(AME and AMEn) and the amino acid(AA) apparent ileal digestibility coefficients(AIDC)of a partially defatted(BSFp) and a highly defatted(BSFh) black soldier fly larvae meal. The experimental diets were: a basal diet and two diets prepared by substituting 250 g/kg(w/w) of the basal diet with BSFp or BSFh, ***: Significant differences were found between BSFp and BSFh meals for ATTDC of the nutrients: BSFp resulted more digestible than BSFh, except for ATTDC of CP which did not differed between meals, while a statistical trend was observed for ATTDC of DM and EE. The AME and AMEn values were significantly(P meals, with higher levels for BSFp(16.25 and 14.87 MJ/kg DM, respectively). The AIDC of the AA in BSFp ranged from 0.44 to 0.92, while in BSFh they ranged from 0.45 to 0.99. No significant differences were observed for the AA digestibility(0.77 and 0.80 for BSFp and BSFh, respectively), except for glutamic acid, proline and serine that were more digestible in the BSFh meal(P meals can be considered as an excellent source of AME and digestible AA for broilers with a better efficient nutrient digestion. These considerations suggested the effective utilization of defatted BSF larvae meal in poultry feed formulation.

关键词： Amino acid Apparent digestibility Black soldier fly meal Broiler chicken Metabolizable energy

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Horizontal transfer of vanA between probiotic Enterococcus faecium and Enterococcus faecalis in fermented soybean meal and in digestive tract of growing pigs

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Journal of Animal Science and Biotechnology 2019年第3期10卷 778-788页

作者： Ning Li Haitao Yu Hongbin Liu Yuming Wang Junyan Zhou Xi Ma Zheng Wang Chengtao Sun Shiyan Qiao State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 10093China Beijing Key Laboratory of Bio-feed Additives China Agricultural UniversityBeijing 10093China National Center for Veterinary Drug Safety Evaluation College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China

Background: The aim of this study was to investigate the intergeneric transfer of vancomycin resistance gene vanA between probiotic enterococci in the fermentation progress of soybean meal and in the digestive tract of growing *** vanA genotype vancomycin resistant *** strain,Efm4,and one chloramphenicol-resistant *** strain,Efs2,were isolated from twenty-nine probiotic basis feed material/additive *** in vitro conjugation,Efm4 and Efs2 were used as starter to ferment soybean *** in vivo conjugation,thirty growing pigs were randomly assigned to five groups(n = 6),treated with a basic diet,or supplemented with 10% fermented soybean meal,1% Efm4,5% Efs2 or a combination of 1% Efm4 + 5% Efs2 for 7 d,*** samples of pigs in each group were collected daily for the isolation and dynamic analysis of Efm4,Efs2 and *** sequence types(STs) of Efm4,Efs2 and transconjugants were analyzed by multilocus sequence typing(MLST).The vanA harboring plasmid in Efm4 and transconjugants was analyzed by S1-pulsed field gel electrophoresis(PFGE)and further verified by multiple ***: The results showed that,in FSBM,transconjugants were detected 1 h after the fermentation,with a conjugation frequency of ~ 10^-3 transconjugants/*** proliferated with Efm4 and Efs2 in the first 8 h and maintained steadily for 10 d till the end of the ***,in vivo experiment showed that transcojugants were recovered in one of six pigs in both FSBM and Efm4 + Efs2 groups,with conjugation frequency of ~ 10^-5 and ~ 10^-4,*** revealed the ST of Efm4,Efs2 and transconjugants was ST1014,ST69 and ST69,respectively.S1-PFGE confirmed the existence of the vanA-harboring,142,988-bp plasmid,which was also a multi-drug resistant plasmid containing Tn1546-like ***: The findings revealed the potential safety hazard existing in the commercial probiotic enterococci in China,because

关键词： Chloramphenicol Digestive tract Enterococci Fermented soybean meal Growing pigs Probiotics Transconjugants vanA Vancomycin

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Isolation of bacteria from fermented food and grass carp intestine and their efficiencies in improving nutrient value of soybean meal in solid state fermentation

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Journal of Animal Science and Biotechnology 2018年第3期9卷 664-673页

作者： Samantha Medeiros Jingjing Xie Paul W.Dyce Hugh Y.Cai Kees De Lange Hongfu Zhang Julang Li Department of Animal Bio Sciences University of Guelph State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural Sciences Animal Science Department Auburn University Animal Health Laboratory University of Guelph College of Life Science and Engineering Foshan University

Background: Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this ***: Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 k Da and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h ***: Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets.

关键词： Allergens Fermentation Food source bacteria Soybean meal

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Effects of dietary cottonseed meal protein hydrolysate on growth,antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis

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Journal of Oceanology and Limnology 2020年第3期38卷 869-882页

作者： CHENG Huihui LIU Wenbin YUAN Xiangyang JIA Erteng ZHANG Dingdong JIANG Guangzhen Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province National Experimental Teaching Center for Animal ScienceCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

One hundred and sixty crabs(average initial weight:51.32±0.08 g)were fed with four experimental diets containing cottonseed meal protein hydrolysate(CPH)at 0%(CPH0,control),0.3%(CPH0.3),0.6%(CPH0.6),and 1.2%CPH(CPH1.2).The experiment results show that no difference was observed in specific growth rate and survival rate of crabs fed with CPH *** content of crabs fed with CPH0.6 diet was significantly reduced than that of the CPH0 *** dismutase and catalase activities of crabs fed with CPH0.6 diet were significantly increased and the difference was not significant between the CPH0.3 and CPH0.6 *** content of CPH0.3 group was significantly lower than that of the CPH0 ***,alkaline phosphatase,and acid phosphatase activities of CPH0.3 diet crabs were significantly higher than that of the CPH0 ***-pyruvic transaminase activity of crabs fed with CPH0.3 diet was significantly decreased compared to the CPH0 *** relative expression levels of Toll1,Toll2,MyD88,LITAF,and ILF-2 of crabs fed with CPH0.3 diet were significantly higher than that of the CPH0 *** expression level of SOCS2 showed an opposite *** CPH perfusion,the expression levels of SOCS2 and Toll1 in intestine at time 3 h and SOCS2 in hepatopancreas at time 18 h increased significantly to the highest *** expression level of Toll2,MyD88,LITAF,decreased at times 6 h,6 h,12 h,respectively,then increased ***,supplementation of dietary CPH could improve antioxidant capacity and immune function;the appropriate supplement dosage of CPH for crab could be 0.3%-0.6%.Furthermore,the short-term CPH stimulation could significantly increase or decline the expression levels of immune-related genes at different times after CPH perfusion.

关键词： Eriocheir sinensis cottonseed meal protein hydrolysate growth body composition immunity antioxidant

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Basal diet and indigestible marker influence apparent digestibilities of nitrogen and amino acids of cottonseed meal and soybean meal in pigs

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Animal Nutrition 2019年第3期5卷 234-240页

作者： Susanto Prawirodigdo Neil J.Gannon Brian J.Leury Frank R.Dunshea Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVictoria3010Australia BPTP Central Java SemarangCentral Java50552Indonesia Biomin Singapore PO Box 2419ToowoombaQLD4350Australia

This study was to determine the apparent ileal nitrogen(N)and amino acid digestibilities of cottonseed meal(CSM)and soybean meal(SBM)in simple carbohydrate-based and more complex wheat-based *** five Large White × Landrace boars(40.9 kg)were randomly allocated to one of 5 dietary treatments:a wheat diet,40% CSM in either a sugar:starch(1:1)or wheat-based diet,or 40% SBM in either a sugar:starch or wheat-based diet for 14 *** diets contained vitamins,minerals,and contained acid-insoluble ash(AIA)and Cr2 O3 as indigestible *** were offered(1,800 g/pig per d)in3 meals/d on d 1-11 and 8 meals/d on d *** d 14,the pigs were fed hourly for 8 *** the 8 th meal,pigs were anaesthetised with isoflurothane,and ileal and faecal digesta sampled from the terminal ileum and rectum before lethal injection of *** ileal digestibility(AID)of N was greater(12.1%)when CSM was fed in the wheat-based diet as compared to the simple carbohydrate-based diet,whereas apparent ileal N digestibility of SBM was slightly lower(-4.5%)in the wheat-based diet as compared to the sugar:starch-based *** ileal amino acid digestibility generally respondeds similarly to ***,while there was a wide difference in apparent N and amino acid digestibilities of SBM and CSM when they were fed in the sugar:starch-based diets,these differences were less apparent when they were fed the wheat-based *** was an apparent net release of N into the hindgut of pigs fed CSM in both base ***,there was quite substantial apparent digestion of N in the hindgut of pigs fed SBM in both base *** use of Cr2 O3 as an indigestible marker resulted in lower apparent ileal and faecal digestibilities than using AIA,particularly for diets containing *** data demonstrate that the basal diet and choice of indigestible marker can substantially influence the estimate of apparent N digestibility,this response can differ for different protein meals.

关键词： Organic matter Pig Wheat Cottonseed meal Soybean meal Digestibility

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Effects of meal size and proximal-distal segmentation on gastric activity

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World Journal of Gastroenterology 2010年第46期16卷 5861-5868页

作者： Madileine F Américo Marjorie V Ietsugu Fernando G Romeiro Luciana A Corá Ricardo B Oliveira José Ricardo A Miranda Department of Physics and Biophysics IBBUNESP-Sao Paulo State University Institute of Biological Sciences and Health ICBSUFMT-University of Mato Grosso Department of Clinical Medicine FMBUNESP-Sao Paulo State University Department of Clinical Medicine FMRPUSP-University of So Paulo UNCISAL-Alagoas State University of Health Sciences

AIM:To evaluate the effects of meal size and three segmentations on intragastric distribution of the meal and gastric motility,by ***:Twelve healthy volunteers were randomly assessed,twice,by *** test meal consisted of 60 or 180 mL of yogurt labeled with 64 MBq 99m Tc-tin *** and posterior dynamic frames were simultaneously acquired for 18 min and all data were analyzed in *** proximal-distal segmentations using regions of interest were adopted for both ***:Intragastric distribution of the meal between the proximal and distal compartments was strongly influenced by the way in which the stomach was divided,showing greater proximal retention after the 180 *** important finding was that both dominant frequencies (1 and 3 cpm) were simultaneously recorded in the proximal and distal stomach;however,the power ratio of those dominant frequencies varied in agreement with the segmentation adopted and was independent of the meal ***:It was possible to simultaneously evaluate the static intragastric distribution and phasic contractility from the same recording using our scintigraphic approach.

关键词： Gastric motility Phasic contractions Proximal stomach Scintigraphy meal size

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