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Bacterial Groups Concerned with maturing Process in Manure Production Analyzed by a Method Based on Restriction Fragment Length Polymorphism Analysis

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Advances in Microbiology 2015年第13期5卷 832-841页

作者： Katsuji Watanabe Naoto Horinishi Kunimasa Matumoto Akihiro Tanaka Kenichi Yakushido Department of Life Environment and Materials Science Fukuoka Institute of Technology Fukuoka Japan National Agricultural Research Center for Kyushu-Okinawa Region National Agriculture and Food Research Organization Koshi Japan National Agricultural Research Center National Agriculture and Food Research Organization Tsukuba Japan

Composting is a biological aerobic decomposition process consisted from different phases. Although the Japanese Standards for manure recommended that it took at least 6 months to complete the maturing phase, there was no reliable ground. In order to find out shortening method of the maturing phase, the microorganisms concerned with a progress of the maturing was determined by using the most probable number method (MPN) and PCR-RFLP of the 16S rDNA, which was found effective to provide numbers and taxonomy of polymyxin B resistant bacterial groups in the former paper [1]. Compared to the numbers after thermophilic phase, those of Actinobacteria, δ-proteobacteria, and the other gram negative bacteria increased to 50 times, 20 times, and 105 times, respectively, after maturing phase, while those of Bacillus spp., and α and β-proteobacteria decreased to 1/10, and 1/105 after maturing phase. Numbers of the other Fumicutes, and γ-proteobacteria remained in the same revel. Actinobacteria, δ-proteobacteria, and the other gram negative bacteria might be concerned with a progress of the maturing phase, because these bacterial groups were detected and enumerated due to their proliferation ability. Although number of Acitinobacteria might be underestimated because of a PCR bias, the method was found effective for the purpose to monitor bacteria actively proliferated in culture medium.

关键词： maturing Phase Manure Production Microchip Electrophoresis Multiple Enzyme Restriction Fragment Length Polymorphism Analysis The Most Probable Number Method

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The Ontogenesis and Ontogenetic Structure of Lagochilus proskorjakovii Ikram (Lamiaceae) Coenopopulations in Nuratau Mountain Range (Uzbekistan)

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American Journal of Plant Sciences 2016年第6期7卷 928-936页

作者： A. K. Akhmedov H. F. Shomurodov Z. B. Nomozova Department of Botany and Plant Physiology Samarkand State University Samarkand Uzbekistan Institute of the Gene Pool of Plants and Animals Academy of Sciences Republic of Uzbekistan Tashkent Uzbekistan

Lagochilus proskorjakovii Ikram is a strict endemic in Nuratau ridge (north-western spurs of the mountain range of the Pamir-Alai). It grows as a part of Amygdalus bucharica and Perovskia scrophulariifolia formation, where the total projective cover of grass does not exceed 20%. In natural conditions growth, ontogeny of L. proskorjakovii is simple and complete. The duration of ontogenesis in the condition of Nuratau is no more than 22 - 23 years. Studied coenopopulations are normal, but are not complete. Coenopopulation self-maintenance is carried out by the seed of renewal. The characteristic spectrum of coenopopulation centered, with a peak in the middle-generative individuals. In most cases, specific developmental spectrum of coenopopulation coincides with the characteristic ones. The absence or small proportion of the young fraction in surveyed of coenopopulation is related to the elimination of young fragile individuals. According to the classification of “delta-omega”, the most of coenopopulations are maturing which confirms the positive state of coenopopulation demography. Studied the ontogeny and structure of adult endemic Nuratau established that the species belongs to caudexum shrubs, perennial that forms the axis of the short multicipital caudex. Buds renewal is at a height of 2 - 3 cm above the substrate. Investigated coenopopulations L. proskorjakovii are successiveness conditions. In addition to the Artemisia juncea + Perovskia scrophulariifolia + Ferula ovina + Stipa hohenackeriana community 1st coenopopulation (CP) mature;the 2nd CP is mature which was a part of the Amygdalus bucharica + Perovskia scrophulariifolia + Prangos pobularia + Bromus scoparius community;3rd CP is maturing being a part of Lonicera nummulariifolia + Perovskia scrophulariifolia + Hypericum scabrum + Bromus danthoniae community and the 4th CP is mature which occurred in Amygdalus bucharica + Ferula varia + Acroptilon repens + Taeniath

关键词： Demographic Characteristics Ontogenetic Spectrum maturing Mature Plant Community Coenopopulation Types

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Effects of non-procedural factors in Brassica napus genetic transformation

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Oil Crop Science 2017年第2期2卷 106-121页

作者： Fang Liu Xiaojuan Xiong Pandi Wang Li Lei Xinhua Zeng Li Zhu Yunjing Li Junling Luo Donghui Fu Ping Fu Gang Wu Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Oil Crops Research InstituteChinese Academy of Agricultural Sciences Wuhan China Department of Agronomy and Plant Genetics University of Minnesota 411 Borlaug Hall 1991 Upper Buford Circle St.Paul Minnesota 55108 USA The Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Agronomy College Jiangxi Agricultural University Nanchang China

Rapeseed (Brassica napus) is the second largest oil crop in the world. However, transformation efficiency of rapeseed still needs to be improved. To evaluate non-procedural factors (e.g. explants, section of explant, marker genes and number of exogenous genes) effects on transformation efficiency, 6-day-old hypocotyl explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring a binary vector using optimized transformation procedure. Results showed that normal maturing variety ‘Zhongshuang 6 (ZS6)’ had the highest overall capacity to produce rooted shoots among 5 common varieties and 6 early maturing varieties, with green callus induction rate 81.45% and shoot regeneration rate 21.66%. Early maturing variety 14M645 has relatively high regeneration rate (4.69%) and one of the shortest growth periods (107.54 d). Data showed that choosing neomycin phosphotransferase II gene (NPTII) as selectable marker led to the best transformation rate (17.38%). Selecting upper hypocotyl segments near cotyledon as explant provided the higest transformation efficiency, with regeneration rate of 25.59% when using NPTII as selectable marker and 22.19% for Bar. B. napus transformed with single gene showed higher transformation frequency than vectors with multiple genes,highlighting difficulty of multiple gene transformation. This work helped to further improve genetic transformation of B. napus by optimizing factors that impact transformation efficiency,and it would ultimately improve research in transgenic B. napus varieties with commercial potential.

关键词： Brassica napus hypocotyl explants genetic transformation regeneration rate early maturing selectable marker gene

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成熟期提高油菜产量的方法(英文)

成熟期提高油菜产量的方法(英文)

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第十二届国际油菜大会

作者： Vlastimil Mikík David Beka Pavel Cihlá Jan Vaák Czech University of Agriculture Department of Crop ProductionKamycká 129165 21 Prague 6-Suchdol Czech Republic

在捷克,10%1%的耕地(28万公顷)用来种植油菜。由于油菜籽成熟不一致,导致最佳收获时间无法确定而造成减产。调节油菜成熟可以选用干燥剂,促进剂和粘合剂。小区试验表明,促进剂Atonik Pro最有效。1.对照2.萘乙酸NAA3.生长素Atonik Pro4.... 详细信息

在捷克,10%1%的耕地(28万公顷)用来种植油菜。由于油菜籽成熟不一致,导致最佳收获时间无法确定而造成减产。调节油菜成熟可以选用干燥剂,促进剂和粘合剂。小区试验表明,促进剂Atonik Pro最有效。1.对照2.萘乙酸NAA3.生长素Atonik Pro4.抗裂角剂spodnam5.生长素Atonik Pro+抗角裂剂spodnam生长素Atonik Pro和抗角裂剂spodnam一起使用会使油菜显著增产,籽粒更饱满,损失减少,还能增加含油量。适量的使用Atonik Pro可以使油菜提前4周收获。

关键词： winter rapeseed stimulation maturing yield harvest losses

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