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Differences of matrix metalloproteinase 2 Expression between Left and Right Ventricles in Response to Nandrolone Decanoate and/or Swimming Training in Mice

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Chinese Medical Journal 2018年第2期131卷 207-212页

作者： Ying Bai Xu-Bo Shi Yu-Qiong Zhang Yue-Li Wang Xin-Yao Liu Maria Asuncion Esteve-Pastor Cardiovascular Center Beijing Tongren Hospital Capital Medical University Beijing 100730 China Department of Echocardiography Beijing Anzhen Hospital Capital Medical university National Clinical Research Center for Cardiovascular Diseases Beijing 100029 China Department of Cardiology Hospital Clinico Universitario Virgen'dela Arrixaca Instituto Murciano de Investigacion Biosanitaria (IMIB-Arrixaca) CIBER-CV Murcia 30120 Spain

Background： matrix metalloproteinase （MMP）-2 plays an important role in the remodeling of left ventricles （LVs） and right ventricles （RVs）. We investigated the differences of MMP-2 expression between LV and RV in response to nandrolone dccanoate （ND）, swimming training （ST）, and combined ND and ST （NS） in mice, based on their structural, functional, and biochemical characteristics. Methods： Totally 28 male C57B1 mice （6 weeks old; 20-23 g） were divided into four groups, including the control （n = 7）, ND （n = 6）, ST （n = 8）, and NS （n = 7） groups. After respective treatments for 8 weeks, echocardiographic examination was used to assess the cardiac structure and function. Van Gieson stain was used to examine the fibrosis of LV and RV in response to different treatments, and Western blotting analysis was performed to explore different MMP-2 expressions between LV and RV in response to ND and/or ST. Analysis of variance was used for comparing the four groups. Results： At 8 weeks, right ventricular dimension/body weight in the ND group was larger than the other three groups （F = 7.12, P 〈 0.05） according to the echocardiographic examination. Fibrosis induced by ND administration was increased more in RV （2.59%） than that in LV （2.21%）. MMP-2 expression of the ND group in RV was significantly greater than the control and NS groups in RV and the corresponding ND group in LV. Conclusion： The experimental data support the hypothesis that ND administration induces greater MMP-2 expression increase in RV compared to LV, leading to consequent RV dilation.

关键词： Fibrosis matrix metalloproteinase 2 Nandrolone Decanoate Swimming Training

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Puerarin, an isoflavone compound extracted from Gegen (Radix Puerariae Lobatae), modulates sclera remodeling caused by extremely low frequency electromagnetic fields

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Journal of Traditional Chinese Medicine 2016年第5期36卷 678-682页

作者： Tian Tian Cai Xiaojing Zhu Huang Department of Ophthalmology Xinhua Hospital Shanghai Jiao Tong University School of Medicine

OBJECTIVE: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen(Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields(ELF-EMFs).METHODS: Human fetal scleral fibroblasts(HFSFs)were divided into 5 groups:(a) untreated controls;(b) cells treated with ELF-EMFs;(c) cells treated with ELF-EMFs and puerarin 0.1 μM;(d) cells treated with ELF-EMFs and puerarin 1 μM;(e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. matrix metalloproteinase-2(MMP-2) activity was measured by gelatin ***-2 and collagenⅠ(COL1A1) m RNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, ***: Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, m RNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 m RNA and protein ***: Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.

关键词： Puerarin Pueraria lobata Sclera Fibroblast matrix metalloproteinase 2 Collagen type Ⅰ Electromagnetic fields

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Therapeutic effect of interleukin-10 on CCI_4-induced hepatic fibrosis in rats

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World Journal of Gastroenterology 2006年第9期12卷 1386-1391页

作者： Yue-Hong Huang Hei-Na Shi Wei-Da Zheng Li-Juan Zhang Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To study the therapeutic effect of exogenous interleuldn-10 on CCl4-induced hepatic fibrosis in rats and its passible mechanisms. METHODS： Fourty-seven SD rats were randomly divided into control group （group N） and CCl4-induced hepatic fibrosis model group （group C）. After CCl4 was given for 9 wk, the model group was divided into three groups. Rats in group H were put to death immediately, rats in group T were treated with IL-10 for another three wk and then put to death, rats in group R recovered after three weeks and were then killed. The degree of hepatic fibrosis was measured by HE staining and histological activity index （HAI）. Histological activity index （HAI）, change of collagen types Ⅰ and Ⅲ were measured by Picrosirius staining. The expression of TNF-α, HHP-2 and TIMP-1 in liver tissue was measured by S-P immunohis tochemistry.RESULTS： CCl4- induced experimental rat hepatic fibrosis model was established successfully. The degree of hepatic fibrosis was markedly lower in group T than in groups H and R, and there was no difference between the two groups. The expression of collagen types I and III was significantly suppressed in group T and was slightly suppressed in groups H and R. The positive levels of TNF-α, HHP-2 and TIHP-1 in group H increased significantly compared to those in group N （P〈0.01）. The positive signals decreased significantly in groups T and R （P〈0.01）, but positive score was significantly lower in group T than in group R （P〈 0.01）. CONCLUS10N： Exogenous IL-10 can reverse CCl4-induced hepatic fibrosis in rats. IL-10 may exert its reversible effects on hepatic fibrosis by blocking CCl4-induced inflammation, inhibiting expression of HHP-2 and TIMP-1 and promoting resolution of collagen types Ⅰ and Ⅲ.

关键词： Animals Carbon Tetrachloride Collagen Type I Collagen Type III Immunohistochemistry Interleukin-10 Liver Liver Cirrhosis Male matrix metalloproteinase 2 Rats Rats, Sprague-Dawley Tissue Inhibitor of metalloproteinase-1 Tumor Necrosis Factor-alpha

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Expression of E-cadherin,beta-catenin,cathepsin D,gelatinases and their inhibitors in invasive ductal breast carcinomas

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Chinese Medical Journal 2007年第18期120卷 1597-1605页

作者： ZHANG Yun-gang DU Juan TIAN Xin-xia ZHONG Yan-feng FANG Wei-gang Department of Pathology Peking University Health ScienceCenter Beijing 100083 China

Background E-cadhedn, beta-catenin, cathepsin D, matrix metalloproteinase （MMP）-2, MMP-9, tissue inhibitors of metalloproteinase （TIMP）-1 and TIMP-2 are all invasion-related proteins. The expression patterns of these proteins in invasive ductal breast carcinomas, and their associations with known clinicopathological parameters, tumor recurrence and expressions of estrogen receptor （ER）, progesterone receptor （PR）, PS2 and c-erbB2 were not well studied in Chinese patients, Methods In a set of 94 invasive ductal breast carcinomas, protein expressions of these molecular markers were investigated by immunohistochemistry, and their associations with known clinicopathological parameters, tumor recurrence and expressions of ER, PR, PS2 and c-erbB2 were also examined. In addition, the interrelationship between the expressions of these proteins were studied. Results Preserved membrane E-cadherin expression was associated with late tumor stage and tumor recurrence, whereas the reduced junctional beta-catenin associated with positive lymph node status and c-erbB2 overexpression. Positive staining of cathepsin D in tumor stromal cells displayed a significant association with late tumor stage. High expression of MMP-2 in cancer cells was associated with large tumor size and PR positive expression. TIMP-2 expression was positively associated with tumor recurrence. In addition, inter-relationship between the expressions of these biomarkers was also assessed, Cathepsin D staining in cancer cells was inversely correlated with its staining in stromal cells, and also inversely correlated with MMP-2 staining in tumor stromal cells. MMP-2 expression in stromal cells displayed an inverse correlation with TIMP-2 expression. MMP-9 expression displayed parallel associations with TIMP-1 and TIMP-2 expression. Conclusion Evaluation of E-cadherin, beta-catenin, cathepsin D, MMP-2 and TIMP-2 expression may be of some help in more accurately predictinq the pro qnosis of invasive ductal

关键词： ductal breast carcinomas E-cadherin beta-catenin cathepsin D matrix metalloproteinase 2

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Lichong decoction reduces matrix metalloproteinases-2 expression but increases Tissue Inhibitors of matrix metalloproteinases-2 ex-pression in a rat model of uterine leiomyoma

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Journal of Traditional Chinese Medicine 2016年第4期36卷 479-485页

作者： Wang Yasong Li Donghua Xu Xin Qian Ruiya Zhang Yalan Huang Yuhua Geng Jianguo Zou Xiaoli Han Hon-gjuan Zhang Wufang School of Traditional Chinese Medicine Capi-tal Medical Universityand School of General Practice andcontinuing EducationCapital Medical UniversityBeijing Xicheng District Desheng Community Health Service Cen-terBeijing 100120China School of Traditional Chinese Medicine Capital Medical UniversityBeijing Key Lab of TCM Collateral Disease Theory ResearchBeijing 100069China Department of Gynecology of Bei-jing Hospital of Traditional Chinese Medicine Beijing100010China The Fourth Ward of Beijing Ob-stetrics and Gynecology Hospjital Capital Medical Universi-tyBeijing Maternal and Child Health Care HospitalBeijing100026China

OBJECTIVE:To study the effect of Lichong decoction(LD) on expression of matrix metalloproteinase-2(MMP-2) and metalloproteinase-2(TIMP-2) in a rat model of uterine leiomyoma(UL).METHODS:UL was induced in rats using exogenous estrogen and *** was administered(p.o.) for 4 weeks,and mifepristone(RU-486)used as a *** observe the effect of LD on the uterine coefficient and uterine transverse diameter,a radioimmunoassay method was used to detect serum levels of sex *** microscopic analyses of pathologic changes in the tissues of UL rats were *** of the proteins of matrix metalloproteinases(MMPs) and tissue inhibitors of metalloproteinases(TIMPs) in uterine tissues was assessed by immunohistochemical staining and western ***:A UL model in rats was established *** reduced uterine weight,uterine coefficient,and uterine transverse diameter compared with untreated *** reduced levels of estradiol,progesterone,follicle-stimulating hormone,and luteinizing hormone in our UL *** improved the pathologic condition of uterine *** of MMP-2 protein decreased to varying extents in LD-treated groups,but TIMP-2 levels were *** appears to reduce MMP-2 expression and increase TIMP-2 expression in UL ***:These data suggest that the mechanism of action of LD on ULs may involve reduction of MMP-2 expression and increase in TIMP-2 expression in rats.

关键词： Leiomyoma matrix metalloproteinase 2 Tissue inhibitor of metalloproteinase-2 Lichong decoction

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Improvement of chronic heart failure by dexamethasone is not associated with downregulation of leptin in rats

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Acta Pharmacologica Sinica 2007年第2期28卷 202-210页

作者： Qin-gui XIA~(2,4) Tao NA~3 Yi-min GUO~2 Yun-tian BI~2 Hai-yan ZHANG~2 De-zai DAI~(3,4)2 Department of Physiology,Wenzhou Medical College,Wenzhou 325003,China 3 Research Division of Pharmacology China Pharmaceutical University,Nanjing 210009,China DepartmentofPhysiology WenzhouMedicalCollegeWenzhou325003China ResearchDivisionofPharmacology ChinaPharmaceuticalUniversityNanjing210009China

Aim:To demonstrate the hypothesis that dexamethasone（Dex）could improvechronic heart failure（CHF）by inhibiting the downstream signaling transductionof leptin but had no influence on the upregulation of leptin and its receptor ***:CHF was induced by left coronary artery ligation for *** rats were treated with Dex 50 ***,histology,reactive oxygen species（ROS）-related parameters,and leptin concentrations inserum were *** mRNA expression of matrix metalloproteinases（MMP）2/9,tissue inhibitor of metalloproteinases（TIMP）1/2,tumor necrosis factor（TNF）-α,and OB-Rb were measured by ***:In the CHF rats,hemodynamicfunctions were deteriorated,which was accompanied with myocardium remodel-ing and histological *** rats showed hyperleptinemia and excessiveROS in the serum,and the upregulation of MMP-2/9,TNF-α,and leptin receptormRNA and downregulation of TIMP-1/2 mRNA in the myocardium compared withthe sham operation *** treatment significantly ameliorated CHF in asso-ciation with the reversion of the abnormalities of MMP-2/9,TIMP-1/2,TNF-α*** Dex had no influence on the hyperleptinemia and the upregulated leptinand its receptor in the myocardium during ***:Dex improves CHFby inhibiting TNF-α,MMP-2,MMP-9,and *** had no effects on upregulatedleptin and its receptor expression and hyperleptinemia induced by CHF.

关键词： leptin chronic heart failure matrix metalloproteinase 9 matrix metalloproteinase 2 tissue inhibitor of metalloproteinase 1 tissue inhibitor of metalloproteinase 2 tumor necrosis factor alpha dexamethasone

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The Effect of 17 β-Estradiol on Invasion by the Ovarian Clear Cell Adenocarcinoma Cell Line ES-2 and the Molecular Mechanism Involved

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Chinese Journal of Clinical Oncology 2005年第4期2卷 717-725页

作者： Jingxin Ding Youji Feng Lianhuo Yin Hongyan Jin Xiaoxia Liu Liangqing Yao Yunyan Sun Department of Gynecology and Obstetrics Shanghai Medical College Fudan University Shanghai 200011 China Department of Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China

OBJECTIVE To assess the effect of 17 β-estradiol（E2） on cell proliferation, cell invasiveness and its regulation of MTA3, Snail and matrix metalloproteinase 2 （MMP-2） expression in the ovarian clear cell adenocarcinoma cell line ES-2, and to further investigate the mechanism involved. METHODS We first investigated expression of ERα, ERβ, PR and E-cadherin of ES-2 cells by RT-PCR and Western blots. Before all experiments, the ES-2 cells were grown in medium depleted of steroid for more than 7 days. Following treatment with 10^-7,10^-8 and 10^-9 M E2, cell viability of the ES-2 cells was determined by the MTT method, and the cell cycle distribution and apoptosis were examined by flow cytometry （FCM）. Invasion and mobility assays were performed using modified Boyden chambers. MTA3, Snail and MMP-2 mRNA expression was measured by RT-PCR, and Snail, MMP-2 protein levels were determined by IHC. MMP-2 activity was assayed by zymography. RESULTS RT-PCR and Western Blots showed that theexpression of ERα and E-cadherin mRNA and protein in the ES-2 cells was negative, while ERβ and PR expression was positive. E2 at 10^-7,10^-8 or 10^-9M stimulated cell proliferation. A level of 10^-8M E2 reduced the proportion of G0-G1 phase cells and increased the proportion of cells in the S phase, but it had no effect on apoptosis. Invasiveness and mobility of the ES-2 cells was significantly increased by 10^-8M E2. Treatment with 10^-8M E2 led to reduced MTA3 mRNA expression, and elevated Snail and MMP-2 mRNA and protein levels. CONCLUSION E2 enhanced invasion by the ES-2 cells. The effects observed maybe mediated by down-regulation of MTA3 and up-reguation of Snail and MMP-2.

关键词： estrogens clear cell adenocarcinoma Snail matrix metalloproteinase 2

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