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Chemical Research in Chinese Universities 2005年第4期21卷 452-456页

作者： XUJia-xi HUANGXu KeyLaboratoryofBioorganicChemistryandMolecularEngineeringofMinistryofEducationofChina DepartmentofChemicalBiologyCollegeofChemistryandMolecularEngineeringPekingUniversityBeijing100871P.R.China

The mass spectrometric fragmentation of 2a,4-disubstituted 5-benzoyl-2a,3,4,5-tetrahydro-2-phenoxy-azeto{[1,2-a]}[1,5]benzodiazepin-1(2H)-ones has been investigated by means of mass-analyzed ion kinetic energy spectrometry under electron impact ionization conditions. All compounds tend to lose different moieties, such as phenoxy, phenoxy and H, and phenoxyketene. Both [M +-PhO] and [M +-PhOH] ions could further lose CO, and the [M +-PhOCHCO] ions could lose propene or styrene, PhCON, PhCOHN, and other fragments.

关键词： Azeto[1,2-a]benzodiazepin-1(2H)-one mass spectrometry Fragmentation Mechanism

mass spectrometry-based proteomic approaches to study pathogenic bacteria-host interactions

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Protein & Cell 2015年第4期6卷 265-274页

作者： Yufei Yang Mo Hu Kaiwen Yu Xiangmei Zeng Xiaoyun Liu Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center College of Chemistry and MolecularEnclineerinq Pekinq University Beijing 100871 China

Elucidation of molecular mechanisms underlying host- pathogen interactions is important for control and treatment of infectious diseases worldwide. Within the last decade, mass spectrometry （MS）-based proteomics has become a powerful and effective approach to better understand complex and dynamic host-pathogen inter- actions at the protein level. Herein we will review the recent progress in proteomic analyses towards bacterial infection of their mammalian host with a particular focus on enteric pathogens. Large-scale studies of dynamic proteomic alterations during infection will be discussed from the perspective of both pathogenic bacteria and host cells.

关键词： mass spectrometry proteomics bacterial infection host-pathogen interactions

mass spectrometry analysis of intact protein N-glycosylation signatures of cells and sera in pancreatic adenocarcinomas

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第1期25卷 51-64页

作者： Mingming XU Zhaoliang LIU Wenhua HU Ying HAN Zhen WU Sufeng CHEN Peng XIA Jing DU Xumin ZHANG Piliang HAO Jun XIA Shuang YANG Center for Clinical mass spectrometry College of Pharmaceutical SciencesSoochow UniversitySuzhou215123China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China State Key Laboratory of Genetic Engineering Department of BiochemistrySchool of Life SciencesFudan UniversityShanghai200438China Department of Clinical Laboratory Center Zhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhou310014China

Pancreatic cancer is among the most malignant cancers,and thus early intervention is the key to better survival ***,no methods have been derived that can reliably identify early precursors of development into ***,it is urgent to discover early molecular changes during pancreatic *** aberrant glycosylation is closely associated with cancer progression,numerous efforts have been made to mine glycosylation changes as biomarkers for diagnosis;however,detailed glycoproteomic information,especially site-specific N-glycosylation changes in pancreatic cancer with and without drug treatment,needs to be further ***,we used comprehensive solid-phase chemoenzymatic glycoproteomics to analyze glycans,glycosites,and intact glycopeptides in pancreatic cancer cells and patient *** profiling of N-glycans in cancer cells revealed an increase in the secreted glycoproteins from the primary tumor of MIA PaCa-2 cells,whereas human sera,which contain many secreted glycoproteins,had significant changes of glycans at their specific *** results indicated the potential role for tumor-specific glycosylation as disease *** also found that AMG-510,a small molecule inhibitor against Kirsten rat sarcoma viral oncogene homolog(KRAS)G12C mutation,profoundly reduced the glycosylation level in MIA PaCa-2 cells,suggesting that KRAS plays a role in the cellular glycosylation process,and thus glycosylation inhibition contributes to the anti-tumor effect of AMG-510.

关键词： Pancreatic cancer Glycosylation Biomarker Glycoproteomics mass spectrometry

mass spectrometry assisted assignments of binding and cleavage sites of Zn(Ⅱ)complex towards oxidized insulin B chain

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Chinese Chemical Letters 2009年第1期20卷 71-75页

作者： Jiang Jiang Qiong Jie Wu Xiang Yang Cai Long Gen Zhu Wen Ji Wang College of Chemistry and Chemical Engineering Fuzhou University Fuzhou 350002 China College of Life Science Fujian Agriculture and Forestry University Fuzhou 350002 China State Key Laboratory of Coordination Chemistry Nanjing University Nanjing 210093 China

The electrospray ionization mass spectrometry investigation showed that the binding sites of [ZnL]^2＋, where L is 2-[bis（2- aminoethyl）amino]ethanol, with oxidized insulin B chain are Phel, His5 and Arg22, which lead to the selective cleavages of the peptide bonds at Phe1-Val2, His5-Leu6, Glu21-Arg22, and Arg22-Gly23 of oxidized insulin B chain.

关键词： Oxidized insulin B chain Zn(Ⅱ) complex mass spectrometry Hydrolytic cleavage

mass spectrometry profiling analysis enables the identification of new modifications in ribosomal RNA

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Chinese Chemical Letters 2023年第3期34卷 157-161页

作者： Xiao-Meng Tang Tian-Tian Ye Xue-Jiao You Xiao-Ming Yin Jiang-Hui Ding Wen-Xuan Shao Meng-Yuan Chen Bi-Feng Yuan Yu-Qi Feng Sauvage Center for Molecular Sciences Department of ChemistryWuhan UniversityWuhan 430072China School of Public Health Wuhan UniversityWuhan 430071China

Ribosomal RNAs(rRNAs) provide the structural framework of ribosomes and play critical roles in protein *** ribosome biogenesis,rRNAs acquire various modifications that can influence the structure and catalytic activity of ***,rRNA modifications in plants have yet to be fully ***,we proposed a method to purify rRNAs by a successive isolation with different strategies,including poly A-based m RNA depletion and agarose gel electrophoresis-based purification,with which highly pure rRNAs could be *** addition,we developed a liquid chromatography-electrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) method to systematically profile and characterize modifications from the isolated highly pure plant 18S rRNA and 25S ***-ESI-MS/MS analysis showed that 10 and 12 kinds of modifications were present in plant 18S rRNA and 25S rRNA,***,among these identified modifications,2 kinds of modifications of N^(2),N^(2)-dimethylguanosine(m^(2,2)G)and N^(6),N^(6)-dimethyladenosine(m^(6,6)A) in 18S rRNA,and 4 kinds of modifications of m^(2,2)G,m^(6,6)A,N7-methylguanosine(m^(7)G) and 3-methyluridin(m^(3)U) in 25S rRNA,were first reported to be present in ***,exposure of Arabidopsis thaliana to cadmium(Cd) led to significant changes of modifications in both 18S rRNA and 25S rRNA of plants,indicating that rRNA modifications play important roles in response to environmental *** discovery of new modifications in plant rRNAs improves the spectra of plant rRNA modifications and may promote the investigation of the functional roles of plant ribosomes in regulating gene expression.

关键词： Ribosomal RNA RNA modification Plant Arabidopsis thaliana Profiling mass spectrometry

mass spectrometry-Based Human Breath Analysis:Towards COVID-19 Diagnosis and Research

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Journal of Analysis and Testing 2021年第4期5卷 287-297页

作者： Zi-Cheng Yuan Bin Hu Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution Institute of Mass Spectrometry and Atmospheric EnvironmentJinan UniversityGuangzhou 510632China

COVID-19 is a highly contagious respiratory disease that can be infected through human exhaled *** breath analysis is an attractive strategy for rapid diagnosis of COVID-19 in a non-invasive way by monitoring breath *** spectrometry(MS)-based approaches off er a promising analytical platform for human breath analysis due to their high speed,specificity,sensitivity,reproducibility,and broad coverage,as well as its versatile coupling methods with different chromatographic separation,and thus can lead to a better understanding of the clinical and biochemical processes of ***,we try to review the developments and applications of MS-based approaches for multidimensional analysis of COVID-19 breath samples,including metabolites,proteins,microorganisms,and *** features of breath sampling and analysis are *** and challenges on MS-based breath analysis related to COVID-19 diagnosis and study are discussed.

关键词： COVID-19 SARS-CoV-2 Breath analysis Breath sampling Multidimensional analysis mass spectrometry

mass spectrometry and mass spectrometry Imaging-based Thyroid Cancer Analysis

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Journal of Analysis and Testing 2022年第3期6卷 235-246页

作者： Hao-Jiang Wang Bo Li Meng-Ting Zhang Chao-Fan Chai Xiao-Rong Li Ning Li Hong Xiao Wei Bian Department of Biochemistry and Molecular Biology School of Basic Medical ScienceShanxi Medical UniversityTaiyuan 030001China College of Pharmacy Shanxi Medical UniversityTaiyuan 030001China Department of Pathology First Hospital of Shanxi Medical UniversityTaiyuan 030001China

Thyroid carcinoma is one of the most common endocrine malignant diseases *** the rapid development of medical technology,early and effective diagnostic methods could be able to improve the survival rate and quality of life of patients suffering from the *** the complexity of cancer,some specific detection method is desired for diagnosis and *** spectrometry imaging(MSI)is an emerging technique for acquiring molecular information from biological tissues without staining and labeling,including qualitative,quantitative and spatial distribution *** the past several decades,MSI has been widely used for pharmacological monitoring,biomolecular imaging of cells and *** this review,we introduce the tumor progression and histological characteristics of thyroid cancer,and focus mainly on the preparation of biological specimens for MSI and mass spectrometry(MS)analysis,as well as the recent progress in MS and MSI-based thyroid cancer *** review thoroughly discusses the importance of MS and MSI for clinical diagnosis,identification and prognosis of thyroid cancer,and provides some new clues for molecular mechanisms research and tumor metastasis.

关键词： Thyroid cancer Metabolic heterogeneity mass spectrometry imaging mass spectrometry Tumor biomarker

mass spectrometry-based phosphoproteomics in cancer research

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Frontiers in Biology 2012年第6期7卷 566-586页

作者： Xiao-Shan YUE Amanda B. HUMMON Department of Chemistry and Biochemistry University of Notre Dame Notre Dame IN 46556 USA

Phosphorylation is one of the most common post translational modifications （PTM）, participating in a large number of processes to regulate cellular functions. Phosphorylation is also one of the key factors in the origin and development of cancer. The rapid development of mass spectrometric-based phosphoproteomic technologies has made it possible for high-throughput identification and quantification of phosphorylation events. In this review, we provide a general introduction and summary of the achievements made in mass spectrometry based phosphoproteomic research, including the approaches for phosphopeptide identification and quantification, as well as instrumentation and data interpretation methods. We also review some discoveries in cancer research made possible by phosphoproteomic analysis technologies.

关键词： phosphoproteomics mass spectrometry cancer research phosphopeptide enrichment quantitative phosphoproteomics data interpretation

mass spectrometry-based proteomics and peptidomics for systems biology and biomarker discovery

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Frontiers in Biology 2012年第4期7卷 313-335页

作者： Robert CUNNINGHAM Di MA Lingjun LI Department of Chemistry University of Wisconsin-Madison 777 Highland Avenue 1101 University Avenue Madison WI 53706 USA School of Pharmacy University of Wisconsin-Madison 777 Highland Avenue Madison WI 53 705-2222 USA

The scientific community has shown great interest in the field of mass spectrometry-based proteomics and peptidomics for its applications in biology. Proteomics technologies have evolved to produce large data sets of proteins or peptides involved in various biologic and disease progression processes generating testable hypothesis for complex biologic questions. This review provides an introduction to relevant topics in proteomics and peptidomics including biologic material selection, sample preparation, separation techniques, peptide fragmentation, post-translational modifications, quantification, bioinformatics, and biomarker discovery and validation. In addition, current literature, remaining challenges, and emerging technologies for proteomics and peptidomics are presented.

关键词： mass spectrometry proteomics peptidomics review post-translational modifications fragmentation separation,protein quantitation sample preparation biomarker discovery validation bioinforrnatics

mass spectrometry-based metabolomic profiling of prostate cancer - a pilot study

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Journal of Cancer Metastasis and Treatment 2019年第1期4卷 1-12页

作者： Krzysztof Ossoliński Joanna Nizioł Adrian Arendowski Anna Ossolińska Tadeusz Ossoliński Jakub Kucharz PawełWiechno Tomasz Ruman Department of Urology John Paul II HospitalKolbuszowa 36-100Poland Faculty of Chemistry Rzeszów University of TechnologyRzeszów 35-959Poland Department of Urooncology Institute of OncologyWarsaw 02-781Poland

Aim:Prostate cancer(PCa)is the most commonly diagnosed non-skin cancer among *** prostate-specific antigen level is used as a standard PCa biomarker for over 20 ***,it has only 33%specificity and 86%sensitivity(for the cutoff value for prostate biopsy of>4 ng/mL).This leads to overdiagnosis and ***-depth insight into PCa metabolomics enables discovery of novel PCa ***:Metabolomic alternation in PCa serum,urine and interstitial fluid was examined using gold-nanoparticle-based laser mass spectrometry *** study included 5 patients who underwent prostate biopsy with positive result,5 patients with negative result and 10 healthy ***:Over two hundred differentiating metabolites(87 in urine,54 in serum and 78 in interstitial fluid)were ***,twenty two and ten metabolites from urine,serum and interstitial fluid respectively showed statistical significant differential abundance between cancer and control ***:Comprehensive metabolomic profile of PCa has been *** of 36 metabolites,20 were identified and should be further evaluated in clinical trials as a potential PCa *** concentration of triglyceride(12:0/20:1)showed over 10 times higher abundance in PCa samples in comparison to healthy controls and is considered the most promising potential biomarker.

关键词： Prostate cancer biomarker metabolomics mass spectrometry