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Molecular cloning of partial 14-3-3 genes in the marine sponge hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

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Science Bulletin 2013年第7期58卷 766-776页

作者： FU WanTao ZHANG JuLin ZHENG ChangBo LIU Jing AN ZhongFu LIU HongWen ZHANG Wei Institute of marine Science-Technology and Environment Dalian Ocean University marine Bioproducts Engineering Group Dalian Institute of Chemical PhysicsChinese Academy of Sciences Key Laboratory of Nearshore marine Environmental Science and Technology of Liaoning Higher Education Graduate School Dalian Ocean University Vocation & Technological College Dalian Ocean University Dalian Environmental Monitoring Center Flinders Centre for marine Bioproducts Development and Department of Medical BiotechnologySchool of MedicineFlinders University

sponges (phylum Porifera) are the phylogenetically oldest metazoa and highly efficient filter *** the marine ecosystem,they are unconditionally exposed to environmental *** the sponge-bacteria interaction is hence of both ecological and biological *** study investigated the specific interaction between the sponge hymeniacidon perleve and the non-infectious bacteria,Escherichia coli and infectious bacteria,Vibrio *** measuring the 14-3-3 mRNA expression of *** partial cDNAs of 14-3-3 proteins and partial 18S RNA in *** were cloned and *** Reverse-transcription real-time PCR,the 14-3-3 mRNA expression of *** was examined when exposed to three common bacteria in aquatic *** and two Vibrio *** different time and *** could efficiently remove *** from the water column without self-infection;however Vibrio at higher dosages infected *** *** was exposed to *** (1.1×10 7 CFU mL-1),*** Ⅱ (1.2×10 6 CFU mL-1) and *** (3.6×105 CFU mL-1) for 6h,the 14-3-3 mRNA expression in the *** Ⅱ and *** groups was down-regulated by 2.67-and 2.36-fold,*** 14-3-3 mRNA expression in the *** group was not significantly ***,no clear trend was observed on the 14-3-3 transcript levels of *** in response to different doses of *** Ⅱ for different *** results demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge ***.

关键词： marine sponge hymeniacidon perleve 14-3-3 mRNA RT-PCR infectious bacteria

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Molecular cloning of partial 14-3-3 genes in the marine sponge hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

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Science China(Technological Sciences) 2013年第3期56卷 766-776页

sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.

关键词： marine sponge hymeniacidon perleve 14-3-3 mRNA RT-PCR infectious bacteria

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