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Antifouling Potential of Bacteria Isolated from a marine biofilm

Journal of Ocean University of China 2014年第5期13卷 799-804页

作者： GAO Min WANG Ke SU Rongguo LI Xuzhao LU Wei Key Laboratory of marine Chemistry Theory and Technology of Ministry of Education Ocean University of China marine Chemical Research Institute

marine microorganisms are a new source of natural antifouling compounds. In this study, two bacterial strains, Kytococcus sedentarius QDG-B506 and Bacillus cereus QDG-B509, were isolated from a marine biofilm and identified. The bacteria fermentation broth could exert inhibitory effects on the growth of Skeletonema costatum and barnacle larvae. A procedure was employed to extract and identify the antifouling compounds. Firstly, a toxicity test was conducted by graduated pH and liquid-liquid extraction to determine the optimal extraction conditions. The best extraction conditions were found to be pH 2 and 100% petroleum ether. The EC50 value of the crude extract of K. sedentarius against the test microalgae was 236.7 ± 14.08 μg mL-1, and that of B. cereus was 290.6 ± 27.11 μg mL-1. Secondly, HLB SPE columns were used to purify the two crude extracts. After purification, the antifouling activities of the two extracts significantly increased: the EC50 of the K. sedentarius extract against the test microalgae was 86.4 ± 3.71 μg mL-1, and that of B. cereus was 92.6 ± 1.47 μg mL-1. These results suggest that the metabolites produced by the two bacterial strains are with high antifouling activities and they should be fatty acid compounds. Lastly, GC-MS was used for the structural elucidation of the compounds. The results show that the antifouling compounds produced by the two bacterial strains are myristic, palmitic and octadecanoic acids.

关键词： marine biofilm bacteria antifouling activity fatty acids

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In situ Colonization of marine biofilms on UNS S32760 Duplex Stainless Steel Coupons in Areas with Different Water Qualities： Implications for Corrosion Potential Behavior

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Journal of marine Science and Application 2014年第3期13卷 346-353页

作者： Luciana V. R. de Messano Barbara L. Ignacio Maria H. C. B. Neves Ricardo Coutinho Fundagao Carlos Chagas Filho de Amparo Pesquisa do Estado do Rio de Janeiro (FAPERJ). Av. Erasmo Braga 118/6 andar Centro. CEP: 20020-000. Rio de Janeiro. RJ. Brazil. Present address: lnstituto do Mar Universidade Federal de Sao Paulo. Av. Almirante Saldanha da Gama 89. Ponta da Praia. CEP11030-400. Santos. SP. Brazil. marine Biotechnology Division Instituto de Estudos do Mar Almirante Paulo Moreira Marinha do Brasil. Rua Kioto 253. Praia dosAnjos. CEP: 28930-000. Arraial do Cabo. RJ. Brazil.

In the presence of biofilms, stainless steels （SS） exhibits an increase in corrosion potential, called ennoblement. In the present study, the corrosion potential （Eco,） behavior of the duplex SS UNS S32760 was recorded simultaneously with the in situ marine biofilm formation in two areas at Arraial do Cabo, Southeastern Brazil. The biofilm at Forno Harbor （an anthropogenically disturbed area） was characterized by higher relative abundances of Bacteria at day 2, followed by diatoms （especially Navicula sp.） on day 10 and dinoflagellates on day 18, whereas no clear trend was recorded at Cabo Frio Island （an undisturbed area）. The ennoblement of Ecor values was site-dependent. In a complementary laboratory assay, biofilms were removed and the Eco values registered in sterile conditions for the subsequent 10 days and corroborated in situ results. Understanding biofilms and SS interactions has important implications for materials science and engineering decisions as well as helping to fill in imnortant gaps in this knowledge.

关键词： duplex stainless steel microbiologically influencedcorrosion corrosion potential ennoblement marine biofilm fieldexperiment

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