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Acta Oceanologica Sinica 2019年第10期38卷 111-115页

作者： Wenjie Yan Jiao Liu Samphal Seng Bin Zhou Kuke Ding Department of Marine Ecology College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China Faculty of Fisheries&Aquaculture Royal University of AgriculturePhnom Penh 12101Cambodia National Institute for Radiological Protection Chinese Center for Disease Control and PreventionBeijing 100088China Key Laboratory of Radiological Protection and Nuclear Emergency Chinese Center for Disease Control and PreventionBeijing 100088China

The response of glutathione S-transferase(GST)in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different *** speaking,express ZmGST in Escherichia coli,then purify the recombinant protein and make the thermal stability ***,the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of *** conclusion,the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range,and affect its resilience in turn.

关键词： Zostera marina antioxidant enzyme glutathione S-transferase (GST) temperature enzyme activity

Isolation, characterization and implications of the bacterial communities associated with established cultures of Chattonella marina(Raphidophyceae) and Skeletonema costatum(Bacillariophyceae)

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Acta Oceanologica Sinica 2019年第1期38卷 128-135页

作者： Jian Wang Zhaohui Wang Jiangang Zhao College of Life Science and Technology Jinan University Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes Jinan University

Cultivable bacteria coexisting in the cultures of two microalgal species, Chattonella marina(Raphidophyceae)and Skeletonema costatum(diatom, Bacillariophyceae), which have been maintained in the laboratory for several years, were examined in this study. Forty-eight and thirty-four cultivable bacterial strains were isolated from different growth stages of C. marina and S. costatum cultures, respectively. A total of twelve unique bacterial phylotypes were isolated. These bacterial phylotypes belonged to Alphaproteobacteria, Gammaproteobacteria,Bacteroidetes, and Actinobacteria. Alphaproteobacteria predominated in phycospheres of both microalgae, and Rhodobacteraceae was the most common family. Bacterial phylotypes were more diversified in cultures of *** than in those of C. marina. Bacterial concentrations increased remarkably after the late stationary phase of C. marina, which might account for the decline in algal cells. One phylotype of S. costatum-associated bacteria had inhibitory effects on Chaetoceros curvisetus(Bacillariophyceae). However, most bacterial phylotypes from cultures of C. marina showed significant inhibition of the growth of C. curvisetus. The results suggested that bacteria associated with C. marina might have some ecological roles in its competition with diatoms.

关键词： phycosphere marine bacteria Chattonella marina Skeletonema costatum Chaetoceros curvisetus algal growth

Biotic and Abiotic Conditions Can Change the Reproductive Allocation of Zostera marina Inhabiting the Coastal Areas of North China

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Journal of Ocean University of China 2019年第2期18卷 528-536页

作者： WANG Ming ZHANG Huanxin TANG Xuexi Qingdao National Laboratory for Marine Science and Technology Department of Marine Ecology College of Marine Life Sciences Ocean University of China

The relationships between reproductive allocation and different factors have been widely recognized for terrestrial plants, but scarcely for marine angiosperms. Focusing on the seagrass Zostera marina, we examined the change in biomass when it was partitioned to growth, vegetative propagation and reproductive allocation of Z. marina. We also evaluated the relationships between reproductive allocation and biotic and abiotic conditions in the coastal areas of north China. The results indicated that the reproductive allocation of Z. marina at the levels of genet, shoot and spathe was positively correlated with shoot characteristics such as height and biomass(P marina, which may affect further the population dynamics.

关键词： Zostera marina reproductive allocation growth dynamics environmental factor

Draft genome sequence of a less-known wild Vigna: Beach pea(V. marina cv. ANBp-14-03)

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The Crop Journal 2019年第5期7卷 660-666页

作者： Awnindra Kumar Singh A.Velmurugan Debjyoti Sen Gupta Jitendra Kumar Ravi Kesari Aravind Konda Narendra Pratap Singh Sibnarayan Dam Roy Utpal Biswas R.Rahul Kumar Sanjay Singh ICAR-Central Island Agricultural Research Institute Port Blair 744101Andaman&Nicobar IslandsIndia Division of Crop Improvement ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia Department of Plant Breeding and Genetics Bhola Paswan Shastri Agricultural CollegePurneaBihar 854302India Division of Plant Biotechnology ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia ICAR-National Research Centre for Biotechnology LBS CentrePusa CampusNew Delhi 110012India

Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family *** is a close relative of cultivated Vigna species such as adzuki bean(***),cowpea(***),mung bean(***),and blackgram(***),and is distributed throughout the *** its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna ***,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were *** analysis identified 50,670 genes with mean coding sequence length 1042 *** analysis revealed highest sequence similarity with ***,followed by *** with the *** genome revealed 16,699 SNPs and 2253 InDels and comparison with the *** genome revealed 17,538 SNPs and 2300 *** our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of *** draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.

关键词： Beach pea Vigna marina NextSeq 500 Whole-genome sequencing Salinity tolerance

Development of a PCR method for detection of Pseudoalteromonas marina associated with green spot disease in Pyropia yezoensis

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Journal of Oceanology and Limnology 2020年第1期38卷 168-176,I0012-I0015页

作者： YANG Huichao YAN Yongwei LI Jie TANG Lei MAO Yunxiang MO Zhaolan Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyKey Laboratory of Maricultural Organism Disease ControlMinistry of Agriculture and Rural Aff airsQingdao Key Laboratory of MaricultureEpidemiology and BiosecurityYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyKey Laboratory of Marine Genetics and Breeding(Ocean University of China)Ministry of EducationQingdao 266003China

Pseudoalteromonas marina is one of the potential pathogens that cause green spot disease(GSD)in Pyropia *** prevent GSD from development and spread,an effective method to detect the pathogen at early GSD infection stages need to be *** this research,PCR methods were established targeting the dnaA gene(encoding chromosome replication initiator protein)and the dnaN gene(encodingβsliding clamp of DNA polymeraseⅢprotein)to detect *** with three primer pairs pws-dnaA2(Forward,5'-ACCGCATTAACGAACTACTCGTG-3';Reverse,5'-TGCCATTACCTACAGCATGG-3'),pcs-dnaN2(Forward,5'-CTTACAACGTTATCAGCGGC-3';Reverse,5'-GTTGAGTATTAAGTGATTGAGTAAGC-3')or pws-dnaN3(Forward,5'-ACTTACAA-CGTTATCAGCGGC-3';Reverse,5'-ACTGCTGTTTGAGTCTGCTAAC-3').Three PCR methods corresponding to the three primer pairs sufficiently distinguished *** from 22 bacterial species,thus resulting in detection limits of 4 to 4×10^2 CFU cells or 2.37×10^1 to 2.37×10^3 fg of *** DNA per PCR *** an artificial infection experiment *** infected with ***,all established PCRs successfully detected *** at early GSD infection *** results show that the established PCRs are specific and sensitive,and are potential for applications in early diagnosis of GSD in Pyropia.

关键词： Pyropia yezoensis green spot disease (GSD) Pseudoalteromonas marina PCR detection early diagnosis

Cobetia marina CICC10367渗透压冲击下羟基四氢嘧啶的合成及释放

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微生物学报 2009年第12期49卷 1590-1595页

作者：郎亚军任亚男柏林张苓花大连海事大学环境科学与工程学院大连116026

【目的】筛选获得耐受渗透压冲击的羟基四氢嘧啶合成菌株,利用"细菌挤奶"工艺提高羟基四氢嘧啶的产率。【方法】从盐池中分离羟基四氢嘧啶合成菌株,并对其进行形态、生理生化及16SrDNA鉴定。考察了培养基及其NaCl浓度对羟基四氢嘧啶合... 详细信息

【目的】筛选获得耐受渗透压冲击的羟基四氢嘧啶合成菌株,利用"细菌挤奶"工艺提高羟基四氢嘧啶的产率。【方法】从盐池中分离羟基四氢嘧啶合成菌株,并对其进行形态、生理生化及16SrDNA鉴定。考察了培养基及其NaCl浓度对羟基四氢嘧啶合成的影响,在优化的条件下利用"细菌挤奶"工艺制备羟基四氢嘧啶。【结果】筛选获得的一株羟基四氢嘧啶合成菌株,鉴定为Cobetia marina CICC10367(*** CICC10367)。NaCl浓度为90g/L的、谷氨酸单钠为唯一碳氮源的培养基有利于羟基四氢嘧啶合成,最高合成量为694.5mg/L。6次渗透压冲击的细菌挤奶,羟基四氢嘧啶的总合成量4179.0mg/L,产率597.0mg/L/d,羟基四氢嘧啶对底物的转化率为80.2mg/g。【结论】筛选获得的菌株*** CICC10367在NaCl诱导下合成羟基四氢嘧啶,该菌株耐受渗透压冲击,利用细菌挤奶工艺显著地提高了该菌株羟基四氢嘧啶的产率和转化率。

关键词： Cobetia marina 羟基四氢嘧啶渗透压冲击细菌挤奶

marina RINALDI From Jtaly with love

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国际服装动态 2006年第9期 74-75页

在意大利北部的小城Reggio Emilia(瑞吉欧．艾米利亚),一向以浓厚的人文精神和艺术氛围闻名遐迩。意大利传统香醋(balsamic vinegar)、巴马芝士(parmesan cheese)和意大利的三色国旗均发源于此。就连世界著名的男高音歌唱家帕华洛帝(Luc... 详细信息

在意大利北部的小城Reggio Emilia(瑞吉欧．艾米利亚),一向以浓厚的人文精神和艺术氛围闻名遐迩。意大利传统香醋(balsamic vinegar)、巴马芝士(parmesan cheese)和意大利的三色国旗均发源于此。就连世界著名的男高音歌唱家帕华洛帝(Luciano Pavarotti)亦是在Reggio Emilia举办的歌唱比赛中夺得冠军,在当地的歌剧院获得首个正式演出机会,从而开始了他的艺术事业。

关键词：艾米利亚男高音歌唱家艺术事业三色人文精神 marina 艺术氛围巴马家族企业纯羊毛

Utilization of Vegetable Waste Juice by Purple Non-Sulfur Bacterium (Afifella marina Strain ME) for Biomass Production

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Journal of Geoscience and Environment Protection 2018年第5期6卷 210-219页

作者： Sujjat Al Azad Wong Hao Jie M. T. B. M. Lal Borneo Marine Research Institute University Malaysia Sabah Jalan UMS Kota Kinabalu Sabah Malaysia

This experiment was conducted to determine the possibility of utilization of vegetable waste juice in the production of purple non-sulfur bacterium Afifella marina biomass. Bacterium was cultured in four diluted such as, 10%, 20%, 30% and 40% (v/v) vegetable waste juices. In addition synthetic 112 media were used as control to compare the growth characteristics of Afifella marine. The bacterium Afifella marina grew well in 10% diluted vegetable waste juice in anaerobic light condition with the highest production of 5.02 g/l dry cell weight in fifth day of culture, whereas the highest total carotenoid production of 0.87 mg/g dry cell weight was recorded in third day of culture. The highest dry cell weight productions recorded in 10% of VWJ was significantly higher compared to four other treatments (F = 14.63;p = 0.00). However, the total carotenoid production of bacteria in 10% VWJ showed no significant difference compared to the carotenoid production in 112 synthetic media, but was significantly higher compared to three other treatments (P = 0.00). The bacterium, Afifella marina has the ability to use vegetable waste juice at certain level for the production of bacterial biomass.

关键词： Afifella marina Vegetable Waste Juice Dry Cell Weight and Carotenoid Production

Physical Properties and Exchange System of Seawater in Royal Yacht Club marina in the Northernmost Gulf of Aqaba

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Natural Science 2019年第4期11卷 106-121页

作者： Riyad Manasrah Thekrayat Al-Majali Faculty of Marine Sciences The University of Jordan Aqaba Jordan Mote Marine Laboratory Sarasota FL USA

This research was aimed at studying the general environmental status of the marina at Royal Yacht Club (RYC), which is located in the Jordanian Gulf of Aqaba. The physical properties (temperature, salinity and density) were measured at surface and bottom water at 19 sites (T1 - T19). Total hydrocarbon was measured at surface at 12 sites (HC1-HC12). The water exchange and residence time were calculated using data of current speed and direction at 16 sites (C1 - C16). The mean values of temperature, salinity and density at surface and bottom waters in the RYC’s marina were 27.13°C ± 0.03°C, 40.51 ± 0.02 psu and 26.83 ± 0.02 kg·m-3, respectively. The results of physical parameters revealed no significant differences among sites, which are all comparable with open waters. The measurements range of total hydrocarbon in the marina was 1.404 mg/l (at site HC11) to 98.56 mg/l (at HC9). In general, all the high values of total hydrocarbon were found at the corners of the marina and at areas with relatively intense boats’ movements. There was no correlation between distribution of total hydrocarbon and temperature (R2 = 0.048;p = 0.49) in the marina. Therefore, the major factors that influence the distribution of total hydrocarbon within the marina are the current system and boats’ movements as well as the location reference to the gate. The residence time of waters inside the marina was 1.32 days. This flushing time is acceptable to secure good environmental conditions inside the marine to avoid stagnant seawater. The mean value of current speed at the RYC’s marina gate with open water was 5.33 ± 2.63 cm/s, which is relatively comparable with current speed in vicinity of study area. In conclusions, the environmental condition of the RYC’s marina is acceptable in term of the residence time and exchange system with open water. Slightly high values of total hydrocarbon were detected, which can be solved by regular cleaning.

关键词： Temperature Salinity Total Hydrocarbon Flushing Time marina Royal Yacht Club Gulf of Aqaba

Assess the Main Factors Affecting the International marina of Taba Heights at the Gulf of Aqaba, Red Sea, Egypt

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Journal of Water Resource and Protection 2014年第1期6卷 22-28页

作者： Mohamed Hamza Hasan Aquatic Environment Department Faculty of Fish Resources Suez University Suez Egypt

The increase in maritime traffic means that ports have to continuously improve their efficiency. This involves reducing the inactivity caused by adverse conditions that affect its operation and maintenance which includes the sedimentation rate, the shallow depth, water current regimes and tidal cycle. This study examines the hypothesis that these factors could affect Taba Heights international marina. The results showed that the harbor offered very good protection against sediment deposition (rate of sediment deposition ranging from 0.2 to 1.9 mg·cm-2·day-1) and currents (two main currents having opposite directions, the first at bearing 261 degree North with mean speed of 3.5 cm/sec., the second at bearing 85 degree North with mean speed of 4.5 cm/sec), while it had a suitable tidal range (0.3 m for neap tidal range and 0.9 m for spring tidal range). The bathymetric study of the marina area showed deeper water at the approaching channel (3.5 to 4.0 meters deep) and inside the marina (3.0 - 4.0 m) than the surrounding marine areas (0.5 to 1.5 meters). The data obtained illustrated that all the investigated factors had limited effect on the operation of Taba Hieghts international marina.

关键词： International marina Current Sedimentation Rate Bathymetry Tide Gulf of Aqaba Red Sea