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A comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses,breeding mares and riding-horses in Italy

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Asian Pacific Journal of Tropical Biomedicine 2013年第3期3卷 169-173页

作者： Karina Mallardo Sandra Nizza Filomena Fiorito Ugo Pagnini Luisa De Martino Department of Pathology and Animal Health Infectious Diseases SectionUniversity of Naples "Federico Ⅱ"

Objective:To investigate the prevalence of methicillin-resistant staphylococci(MRS)which is a potencial risk factor of transmission between animals and humans in different types of horses(harness racing-horses,breeding mares and riding-horses)and to compare the antimicrobial resistance of the isolates.Methods:A total of 191 healthy horses,housed at different locations of the Campania Region(Italy),were included in the study.Nasal swab samples were collected from each nostril of the horses.The mecA gene was detected by a nested PCR technique.Antibiotic susceptibility was tested for each isolate.Results:MRS was isolated from nasal samples of 68/191(35.6%;95%CI:28.9%-42.9%)healthy horses.All isolates were coagulase-negative with the exception of two coagulase-positive MRS strains,identified as Staphylococcus aureus and Staphylococcus pseudintermedius.2/83(2.4%;95%,CI:0.4%-9.2%).Interestingly,both coagulase-positive MRS isolates were from harness racing-horses.These horses also presented a significantly higher positivity for MRS(53.3%;95%CI:40.1%-66.1%)than the breeding mares and riding-horses groups.Antibiotic susceptibility testing showed difference between isolates due to different origins except for an almost common high resistance to aminopenicillins,such as ampicillin and amoxicillin.Conclusions:It can be concluded that harness racing-horses may act as a significant reservoir of MRS as compared to breeding mares and riding-horses.

关键词： Methicillin-resistant staphylococci mares Harness racing-horse Riding-horse Nasal swabs

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Endoscopic monitoring the estrous phase of Arabian mares with assaying its biopsy

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Asian pacific Journal of Reproduction 2017年第3期6卷 140-143页

作者： Mohamed Fathi Haithem AMFarghali Nagla AAbdelkader Khattab MA El Karmoty AF Department of Theriogenoloy Faculty of Veterinary MedicineCairo UniversityEgypt Department of Surgery Anesthesiology and RadiologyFaculty of Veterinary MedicineCairo UniversityEgypt Department of Cytology and Histology Faculty of Veterinary MedicineCairo UniversityEgypt Department of Anatomy and Embryology Faculty of Veterinary MedicineCairo UniversityEgypt

Objective:To evaluate the changes occur in the vagina, cervix and endometrium during the pre- ovulatory period in Arabian mares by using endoscope as a diagnostic tool as well as the availability of taking an endoscopic biopsy during this stage.Methods:Ten cyclic and healthy arabian mares were subjected to ultrasound scanning of their ovaries to estimate the diameter of the preovulatory follicles, collection of blood samples for estimating the hormonal levels (Estradiol-17β and progestrone) for confirming the estrous phase, endoscopic monitoring their vagina, cervix and endometrium, the availability of taking biopsical samples and histological examination of the obtained samples.Results:The obtained data revealed that, the mean diameter of the preovulatory follicles was (3.95±0.34) cm, the plasma estradiol and progestrone levels were (85.98±5.26) pg/mL and (0.73±0.23) ng/mL, respectively, endoscopic monitoring the vagina, cervix and endometrium revealed hyperemic vaginal mucous membrane, soft and opened cervical ostium with abundant estrous mucous and folding, hyperemic and edematous appearance of the endometrium, respectively and the histological features of the biopsical endometrial samples revealed that, The lamina propria-sub mucosa was packed with simple or branched tubular glands lined with simple columnar epithelium showing oval or rounded dark nuclei with different degrees of secretory activities of the glands.Conclusions: The endoscope proved to be a reliable tool for confirming the estrous phase in mares and monitoring the gross features of the vagina, the cervix and the endometrium, in addition to the availability of taking biopsical smears.

关键词： Arabian mares Endoscope Estrous

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Collagen types in relation to expression of estradiol and progesterone receptors in equine endometrial fibrosis

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Advances in Bioscience and Biotechnology 2013年第4期4卷 599-605页

作者： Diego Lunelli Silvana Maris Cirio Selene C. Leite Carlos Eduardo Camargo Luiz Ernandes Kozicki Federal University of Paraná Paraná Brazil Pontifical Catholic University of Paraná Paraná Brazil Veterinary Medicine College Pontifical Catholic University of Paraná Paraná Brazil Veterinary Medicine College University Centrum of Evangelica Curitiba Brazil

The aim of this study was to determine the influence of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of 25 crossbred mares were studies. Two endometrial samples were collected from each mare,1 inthe estrus and1 inthe diestrus phase. The samples were classified according to histological changes. Collagen was typed and quantified using the picrosirius red histochemical technique, and steroid receptors were identified by immunohistochemistry. The results showed a predominance of collagen type III in all the endometrial samples. The expression of estrogen (RE2) and progesterone (RP4) receptors varied according to the estrous cycle. RE2 and RP4 expression varied in the estrus and diestrus phases;there was no influence of collagen I or II on receptor expression.

关键词： Endometriosis mares Picrosirius Red Immunohistochemistry Estradiol Receptors Progesterone Receptors

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