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Effects of Cow’s Milk on Reproduction in ICR male mice

Biomedical and Environmental Sciences 2009年第2期22卷 161-163页

作者： YU-XIA MA NAOYUKI EBINE KAZUO AOKI MASAHIRO KUSUNOKI AND JUNICHI MISUMI Department of Human Environmental and Social Medicine Division of Preventive Medicine Faculty of Medicine Oita University 1-1 ldaigaoka Hasama-machi Oita 879-5593 Japan Department of Nutrition and Food Hygiene College of Public Health Hebei Medical University Shijiazhuang 050017 Hebei China

Objective To study the effects of Cow＇s milk on the reproduction in male mice. Methods Twenty-four male mice were divided randomly into two groups： milk group （M） and control group （C）. Each mouse was given 10 mL milk per day from 4 to 16 weeks in the group M. At the age of 17 weeks, all the mice were sacrificed. Results Serum testosterone was decreased in the group M （P=0.037）. No significant difference was found in weight of testes, seminal vesicle or adrenal gland of mice between the groups C and M. However, the weight of seminal vesicle decreased when expressed in g/100 g body weight in the group M. Epididymal sperm concentration, motility, morphology, and sperm head number were not affected by milk. Conclusion Cow＇s milk has adverse effects on the reproductive system in ICR male mice. Further studies are needed to clarify the specific effects of milk on reproductive health.

关键词： Cow's milk male mice Reproductive health

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Effects of Saikokaryukotsuboreito on Spermatogenesis ant Fertility in Aging male mice

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Chinese Medical Journal 2016年第7期129卷 846-853页

作者： Zhi-Jun Zang Su-Yun Ji Ya-Nan Zhang Yong Gao Bin Zhang Department of Infertility and Sexual Medicine The Third Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510630 China Department of Dermatology Guangdong Provincial Dermatology Hospital Guangzhou Guangdong 510091 China Reproductive Medicine Centre and Guangdong Provincial Key Laboratory of Reproductive Medicine The First Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510080 China

Background： Aspermia caused by exogenous testosterone limit its usage in late-onset hypogonadism （LOH） patients desiring fertility. Saikokaryukotsuboreito （SKRBT） is reported to improve serum testosterone and relieve LOH-related symptoms. However, it is unclear whether SKRBT affects fertility. We aimed to examine the effects of SKRBT on spermatogenesis and fertility in aging male mice. Methods： Thirty aging male mice were randomly assigned to three groups, mice were orally administered with phosphate-buffer solution or SKRBT （300 mg/kg, daily） or received testosterone by subcutaneous injections （10 mg/kg, every 3 days）. Thirty days later, each male mouse was mated with two female mice. All animals were sacrificed at the end of 90 days. lntratesticular testosterone （ITT） levels, quality of sperm, expression of synaptonemal complex protein 3 （SYCP3）, and fertility were assayed. Results： In the SKRBT-treated group, ITT, quality of sperm, and expression of SYCP3 were all improved compared with the control group （ITT： 85.50 ＋ 12.31 ng/g vs. 74.10 ±11.45 ng/g, P = 0.027; sperm number： [ 14.94 ± 4.63] × 106 cells/ml vs. [8.79±4.38] × 106 cells/ml, P = 0.002; sperm motility： 43.16 ± 9.93% vs. 33.51 ± 6.98%, P = 0.015; the number of SYCP3-positive cells/tubule： 77.50 ± 11.01 ng/ml vs. 49.30 - 8.73 ng/ml, P 〈 0.001 ; the expression of SYCP3 protein： 1.23± 0.09 vs. 0.84 ± 0.10, P 〈 0.001 ）, but fertility was not significantly changed （P 〉 0.05, respectively）. In the testosterone-treated group, ITT, quality of sperm, and expression of SYCP3 were markedly lower than the control group （ITT： 59.00 ±8.67, P = 0.005; sperm number： [4.34 ± 2.45] 100 cells/ml, P = 0.018： sperm motility： 19.53 ± 7.69%, P = 0.001 ; the number of SYCP3-positive cells/tubule section 71.98 ：k 8.88%, P= 0.001 ; the expression of SYCP3 protein： 30.00 ± 11.28, P 〈 0.001 ; the percentage of SYCP3-positive tubules/ 0.71± 0.09, P 〈 0.001 ）, and fertility was also suppressed （P 〈 0.05, respectively）. Conclusion：

关键词： Late-onset Hypogonadism male mice Saikokaryukotsuboreito Spermatogenesis Testosterone Synthesis

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PTA wastewater molecular toxicity detected with gene chip

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Journal of Environmental Sciences 2006年第3期18卷 514-518页

作者： ZHU Cheng-jun CHENG Shu-pei ZHANG Xu-xiang LANG You-zhe SUN Shi-lei GU Ji-dong ZHAO Da-yong PAN Wen-yang YU Hong-xia State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China Department of Ecology and Biodiversity University of Hong Kong Hong Kong China

The purified terephthalic acid （PTA） petrochemical wastewater molecular toxicity detected by use of Mouse Genome 430A 2.0 GeneChip was conducted in this research. The toxic dose to male mice was 0.03 g/（kg, d） of PTA in the wastewater. The mice liver total RNA was isolated as the temple for synthesis of eDNA and then the cDNA as the temple for synthesis of cRNA. Hybridizing the cRNA with the target genes on the gene chip, there were 232 genes expression levels up-regulated and 74 genes down-regulated discovered obviously. The foremost 40 genes for both the highest and the lowest expression levels involved endogenetic steroid and hormone metabolism, immune system, the leukocyte activity and inflammation, detoxification in liver, reproduction and growth hormone, regulation immune factors of anti-tumor and anti-infection and cancer to the mice sampled. The data suggest the PTA wastewater contained over 5 aromatics and their toxicities integrated were much higher than the pure chemical PTA. And the pure chemical PTA toxicities data cannot be used to evaluate the toxicity of the PTA wastewater instead.

关键词： gene chip PTA wastewater male mice molecular toxicity predication of security

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