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Development of an Inoculation Technique for Rapidly Evaluating maize inbred lines for Resistance to Stalk Rot Caused by Fusarium spp. in the Field

Development of an Inoculation Technique for Rapidly Evaluati...

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中国植物病理学会2024年学术年会

作者：何影李永刚 College of Plant Protection Northeast Agricultural University

maize(Zea mays L.) stalk rot,primarily caused by the soilborne fungal pathogen Fusarium spp.,is a major crop disease worldwide that adversely affects maize quality and *** study was initiated with the aim to develop and utilize a rapid continuous injection inoculation technique for evaluating maize inbred lines for resistance to Fusarium *** field conditions,which could help in the identification and development of new sources of and strategies for host resistance to manage the *** injection inoculation is a rapid,stable,and simple method that can evaluate the resistance of maize inbred lines to Fusarium stalk rot(FSR) within 20 days,which is a significant improvement over the traditional methods that require a longer *** verify the feasibility and reliability of the inoculation method,Fusarium graminearum,***,and *** were isolated,identified,and inoculated into maize at the six-leaf stage(V6) by a veterinary adjustable bottle continuous vaccination *** results showed that,of a total of 97 inbred maize lines,6(6.2%)showed high resistance to maize stalk rot,20 showed resistance(20.6%),32 were susceptible(33.0%),and 39 were very susceptible(40.2%).Based on simple sequence repeat markers,an analysis of molecular variance indicated a significant correlation between population of the inbred maize line and resistance to FSR(P=0.001),which suggests that the resistance to FSR is a heritable ***,this study provided a systematic,rapid,stable,and simple identification method for maize inbred lines resistant to FSR in the field,which could be used to select resistant lines for breeding programs and to develop strategies for managing the *** the same time,this method is also suitable for geneticdiversity analysis of maize inbred lines resistant to FSR,which can help in understanding the genetic basis of resistance to the disease.

关键词： continuous injection method Fusarium spp. maize inbred lines resistance stalk rot

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Efficient and genotype independent maize transformation using pollen transfected by DNA-coated magnetic nanoparticles

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Journal of Integrative Plant Biology 2022年第6期64卷 1145-1156页

作者： Zuo-Ping Wang Zhong-Bao Zhang Deng-Yu Zheng Tong-Tong Zhang Xiang-Long Li Chun Zhang Rong Yu Jian-Hua Wei Zhong-Yi Wu Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology Institute of BiotechnologyBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Capital Normal UniversityBeijing 100048China

Current gene delivery methods for maize are limited to specific genotypes and depend on timeconsuming and labor-intensive tissue culture ***,we report a new method to transfect maize that is culture-free and genotype *** enhance efficiency of DNA entry and maintain high pollen viability of 32%-55%,transfection was performed at cool temperature using pollen pretreated to open the germination aperture(40%–55%).Magnetic nanoparticles(MNPs)coated with DNA encoding either red fluorescent protein(RFP),β-glucuronidase gene(GUS),enhanced green fluorescent protein(EGFP)or bialaphos resistance(bar)was delivered into pollen grains,and female florets of maize inbred lines were *** fluorescence was detected in 22%transfected pollen grains,and GUS stained 55%embryos at 18 d after *** fluorescence was detected in both silk filaments and immature *** T1 generation of six inbred lines showed considerable EGFP or GUS transcripts(29%–74%)quantitated by polymerase chain reaction,and 5%–16%of the T1 seedlings showed immunologically active EGFP or GUS ***,1.41%of the bar transfected T1 plants were glufosinate resistant,and heritable bar gene was integrated into the maize genome effectively as verified by DNA *** results demonstrate that exogenous DNA could be delivered efficiently into elite maize inbred lines recalcitrant to tissue culture-mediated transformation and expressed normally through our genotype-independent pollen transfection system.

关键词： exogenous genes genotype independence magnetic nanoparticles maize inbred lines pollen transfection pollen aperture

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