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Mycoplasma pneumoniae macrolide resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?

Biomedical and Environmental Sciences 2020年第12期33卷 916-924页

作者： DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei Beijing Tropical Medicine Research Institute Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Disease Capital Medical UniversityBeijing 100050China Qingdao Women and Children`s Hospital Qingdao 266000ShandongChina Department of Pediatrics Peking University Third HospitalBeijing 100191China Department of Pediatrics Civil Aviation General HospitalBeijing 100123China Department of Pediatrics Beijing Chao-yang HospitalCapital Medical UniversityBeijing 100043China Department of Pediatrics Emergency General Hospital(China Meitan General Hospital)Beijing 100028China Department of Respiratory Beijing Children’s HospitalCapital Medical UniversityBeijing 100045China Department of Pediatrics Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China

Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric *** Real-time quantitative polymerase chain reaction(PCR)was used to identify ***,and MLVA was *** domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point *** also investigated the activities of antibiotics against *** isolates in *** The PCR detection rate of *** in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type *** other types were *** strains were resistant to levofloxacin or *** In 2016,a specific decrease in the macrolide resistance rate occurred in *** detection rate and macrolide resistance rate of outpatients are lower than those of *** A2063 G mutants *** have high levels of resistance to erythromycin and *** primary MLVA type is 4-5-7-2,followed by *** other MLVA types were *** strains resistant to tetracycline or levofloxacin were found in vitro.

关键词： Culture macrolide resistance MLVA type Mycoplasma pneumoniae

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Identification of Campylobacter jejuni and determination of point mutations associated with macrolide resistance using a multiplex TaqMan MGB real-time PCR

Identification of Campylobacter jejuni and determination of ...

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中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

作者：郝海红刘杰 KUANG Xiuhua DAI Menghong CHENG Guyue WANG Xu PENG Dapeng HUANG Lingli AHMAD Ijaz REN Ni LIU Zhenli WANG Yulian 袁宗辉 National Reference Laboratory of Veterinary Drug Residues(HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural University MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural University

The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance.A multiplex fluorescence real-time PCR assay was developed based on TaqMan minor groove binder(MGB) *** VS1-MGB probe was designed based on the VS1 gene and was used to identify Campylobacter *** 23 S rDNA-MGB probe was designed to distinguish macrolide resistance mutations in 23 S rDNA,while 57D-MGB and 74D-MGB were designed to detect resistance mutations in ribosomal protein *** specificity and accuracy of our method were identical to the conventional biochemical tests,mapA PCR,minimum inhibitory concentration(MIC) determination and DNA *** linear detection limit of the method was 003 ng genomic DNA and three colony formation unit(CFU) per *** 6 of 18 cases,the nature of Erythromycin resistance could be correctly determined from natural isolates;absence of the tested mutations was demonstrated in the remaining four resistant ***,a multiplex TaqMan MGB real-time PCR assay with high specificity and accuracy was developed to simultaneously identify *** and detect the gene mutations associated with macrolide *** multiplex method can potentially simplify the identification of Camp,jejuni and determine macrolide resistance due to mutations in 23 S rDNA or ribosomal protein *** method has a potential for application in different research areas and molecular surveillance.

关键词： C.jejuni macrolide resistance MGB point mutation real-time PCR.

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Surveillance of Mycoplasma pneumoniae infection among children in Beijing from 2007 to 2012

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Chinese Medical Journal 2014年第7期127卷 1244-1248页

作者： Zhao Hanqing Li Shaoli Cao Ling Yuan Yi Xue Guanhua Feng Yanling Yan Chao Wang Liqiong Fan Zhaoyang Sun Hongmei Department of Bacteriology Capital Institute of PediatricsBeijing 100020 China Affiliated Children's Hospital of Capital Institute of Pediatrics Beijing 100020 China

Background Mycoplasma pneumonia （***） is one of the key pathogens of community-acquired pneumonia.A global pandemic of *** has occurred since *** aim of this study was to survey the prevalence of *** in children in Beijing from *** A total of 3 073 clinical specimens were obtained from pediatric patients with respiratory tract infections from January 2007 to December 2012,and examined by nested polymerase chain *** products were visualized by 2％ agarose gel electrophoresis,positive products sequenced,and compared with reference sequences in *** resistance-associated mutations were also detected for some positive *** Of the 3 073 specimens,588 （19.13％） were positive for ***,12.4％ of which were accompanied by viral *** rates for *** were highest in 2007 and 2012,showing a significant difference when compared with other *** tended to occur in autumn and winter and positive rates were significantly higher for children aged *** rate of macrolide resistance-associated mutations was 90.7％,and the predominant mutation was an A→G transition （89.92％） at position 2063 in domain V of the 23S rRNA *** *** outbreaks occurred in 2007 and 2012 in pediatric patients in Beijing,which is consistent with the global prevalence of *** can cause multi-system infections in children,and may be accompanied with viral *** determined that school-age children are more susceptible to this disease,particularly in autumn and *** mutations associated with macrolide resistance were very common in ***-positive specimens during this period in Beijing.

关键词： children Mycoplasma pneumoniae prevalence co-infection macrolide resistance

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Overview of the epidemic characteristics of Mycoplasma pneumoniae infection around COVID pandemic

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iLABMED 2023年第3期1卷 148-157页

作者： Hongmei Sun Li Xiao Department of Bacteriology Capital Institute of PediatricsBeijingChina Department of Medicine University of Alabama at BirminghamBirminghamAlabamaUSA

Mycoplasma pneumoniae(***)is a cell wall‐less respiratory pathogen causing community‐acquired pneumonia and extrapulmonary *** is transmitted through close contact and shows periodic regional *** coronavirus disease(COVID‐19)pandemic interfered with the global spread of ***.A large‐scale post‐COVID outbreak is currently ongoing in *** help physicians better understand and manage this epidemic,we provide this review summarizing current knowl-edge on the pathogenesis,epidemic characteristics,macrolide resistance,diagnostic methods,and clinical treatment strategies for this pathogen.

关键词： diagnosis epidemic genotype macrolide resistance Mycoplasma pneumoniae treatment

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