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IMMUNORESPONSES OF HUMANIZED SCID MICE TO HUMAN lung cancer cells

Chinese Medical Sciences Journal 1996年第2期11卷 110-112页

作者：陈力真王树蕙张云王世真 Institute of Basic Medical Sciences CAMS & PUMC Beijing.

HuPBLSCID mice were used to explore how they would response to human tumor cells of *** 80llMLC cells appeared to be fetal to the the mice due to the production of human TNF- The hupBL-SCID mice did not generate any noticeable amount of specific human immunoglobulin either by single immunization with living 801/MLC cells or by repeated immunization with irradiated 80llMLC cells. Our preliminary experiments with huPBL-SCID mice showed that such chimeras would be a very useful models for tumor immunological researches.

关键词： huPBL-SCID mice lung cancer cells immunoglobulin

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The effects of the same target on malignant proliferation of human lung cancer cells with different expression levels of COX-2 protein

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The Chinese-German Journal of Clinical Oncology 2010年第3期9卷 125-132页

作者： Weiying Li Hui Wang Baitang Lai Xuehui Yang Chunyan Zhang Beijing Tuberculosis and Thoracic Tumor Research Institute Beijing 101149 China

Objective: The aim of the study was to explore the effects of the same target (si-10) on lung cancer cells with different expression levels of cyclooxygenase-2 (COX-2) protein by RNAi and malignant proliferation of these cells. Methods: COX-2 was selected as the target and one siRNA expression vector with the best effect was selected and thought as the subject from three COX-2 siRNA expression vectors with human U6 promoter. The siRNA expression vector (psi-10) and the vacant vector (pEGFP) were transfected into these cells with different COX-2 expression states (801D, A549 and LTEP-A2) with lipofectamine respectively and the transfected cell strains were constructed. The change of COX-2 expression levels was examined by Western blot and RT-PCR. The effects on the proliferation of lung cancer cells were studied by cell growth curve and clonogenic assay. Results: The siRNA and U6 promoter were validated by PCR, restriction endonucleases identification and DNA sequencing and BLAST alignment and cloned into the pEGFP vector. The cell strains transfected that 801D was used as maternal line were named as 801D-p and 801D-10 respectively. The cell strains transfected that A549 was used as maternal line were named as A549-p and A549-10 respectively. The cell strains transfected that LTEP-A2 was used as maternal line were named as LTEP-A2-p and LTEP-A2-10 respectively. These cells transfected pEGFP (801D-p, A549-p and LTEP-A2-p) had the expression of GFP and 801D-10, A549-10 and LTEP-A2-10 cells had not in 24, 48 and 72 hours after transfected. The results of RT-PCR and Western blot showed the siRNA expression vector produced marked effects in two cells (A549 and LTEP-A2) expressing COX-2 and the expression of COX-2 was inhibited. But the inhibited effects were differ- ent and the expression of COX-2 was more inhibited obviously in LTEP-A2 cells than in A549 cells though the expression of COX-2 was also inhibited obviously in A549 cells. In contract to their maternal line, t

关键词： cyclooxygenase-2 (COX-2) lung cancer cells RNAi malignant proliferation

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Reduced apurinic/apyrimidinic endonuclease(APE1) activity enhances the antitumor activity of oxymatrine in lung cancer cells

Reduced apurinic/apyrimidinic endonuclease(APE1) activity en...

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中国生物化学与分子生物学会2016年全国学术会议

作者： Xu Wenya Lin Ziying Li Chunyan Liu Gang Clinical Research Center Affiliated Hospital of Guangdong Medical University

lung cancer is the leading cause of cancer-related deaths worldwide and is associated with a very poor *** exerts anti-tumor effects by inducing apoptosis and inhibiting the proliferation of different cancer cells;however,the anticancer effects and mechanism of action of oxymatrine have not been evaluated sufficiently in human lung cancer cells.

关键词： Oxymatrine apurinic endonuclease 1 apoptosis lung cancer cells

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Inhibition　of　GST－π　Expression　by　Retrovirus－mediated　Antisense　RNA　Transfection

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Developmental and Reproductive Biology 1995年第1期4卷 70-76,T001页

作者：周中军金顺钱罗贤懋陈凤魏慧娟

Human antisense GST-πRNA was transferred into adriamycine-treatedadenocarcinoma cell of lung. The over-expression of GST-πgene wasinhibited and the total GST activity towards CDNB, the test substrate, wasdecreased. Transfected cells showed elevated sensitivity to adriamycine ascompared with un-transfected cells. The results suggest that GST-π plays arole in drug resistance of human adenocarcinoma of lung, and inhibition ofGST eazymes might partially restore the sensitivity of cancer tochemotherapy.

关键词： GST-π Antisense RNA Gene transfection lung cancer cells

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Death Receptors 4 and 5 Play Opposite Roles in the Regulation of Geranylgeranyltransferase I Inhibition-mediated Augmentation of TRAIL-inducing Ligand-induced Apoptosis

Death Receptors 4 and 5 Play Opposite Roles in the Regulatio...

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2011医学科学前沿论坛第十二届全国肿瘤药理与化疗学术会议

作者： ShrutiM.Raja YuriK.Peterson FadloR.Khuri, Department of Hematology and Medical Oncology Winship Cancer InstituteEmory University School of MedicineAtlanta Georgia Department of Pharmacology and cancer Biology Duke University Medical CenterDurhamNorth Carolina 27710

Geranylgeranyltransferase I(GGTase I) has emerged as a cancer therapeutic ***,small molecules that inhibit GGTase I have been developed and exhibit encouraging anticancer activity in preclinical ***, their underlying anticancer mechanisms remain *** we have demonstrated a novel mechanism by which GGTase I inhibition modulates *** of GGTase I by GGT1-298 induced apoptosis and augmented tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-induced apoptosis in human lung cancer ***-298 induced the expression of DR4 and DR5 and reduced c-FLIP *** expression of c-FLIP or knockdown of DR5 expression protected cells from induction of apoptosis by GGTI-298/TRAIL,indicating that induction of DR5 and downregulation of c-FLIP mediate augmentation of TRAIL-induced apoptosis by GGTase I ***,DR4 knockdown sensitized cancer cells to GGTI298/TRAIL-induced *** combination of GGTI-298 and TRAIL was more effective than each single agent in decreasing the levels of IκBαand p-Akt,implying that GGTI298/TRAIL activates NF-κB and inhibits ***,knockdown of DR5,but not DR4,prevented GGTI298/TRAIL-induced IκBαand p-Akt reduction,suggesting that DR5 mediates reduction of IκBαand p-Akt induced by GGTI298/*** contrast,DR4 knockdown further facilitated GGTI298/TRAIL-induced p-Akt ***,it appears that DR4 plays an opposite role to DR5 in regulation of GGTI/TRAIL-induced apoptotic signaling.

关键词： GeranylgeranyltransferaseⅠinhibitors death receptor 4 death receptor 5 c-FLIP TRAIL lung cancer cells

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