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Genetic analysis and molecular detection of resistance to chlorpyrifos mediated by the A216S substitution in acetylcholinesterase-1 in the plant bug Apolygus lucorum

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Insect Science 2020年第6期27卷 1224-1232页

作者： Kai-Ran Zuo Yi-Hua Yang Yi-Dong Wu Shu-Wen Wu College of Plant Protection Nanjing Agricultural UniversityNanjingChina

The green plant bug Apolygus lucorum is a major pest of Bacillus thuringiensis cotton in ***,we reported that chlorpyrifos resistance in a laboratory-selected strain of ***(BZ-R)is associated with the homozygosis of an allele in the ace-1 gene encoding an alanine to serine substitution at position 216 of *** we describe the results of crosses between the resistant BZ-R strain(41-fold to chlorpyrifos)and the unselected susceptible BZ-S strain homozygous for the wild type alanine allele at position *** to chlorpyrifos was inherited as a semi-dominant trait mainly controlled by a single autosomal gene and co-segregates strongly but not completely with the serine substitution in *** bioassays and enzyme assays showed that minor contributions to resistance are also made by enhanced cytochrome P450 and carboxylesterase activities.A survey of 25 field populations from five Chinese provinces showed strong positive correlations between 50%lethal concentration against chlorpyrifos and S216 allele and genotype frequencies,although the most tolerant populations still only show 40%-50%S216 allele *** results above provide important information for designing effective resistance monitoring and management strategies for *** in China.

关键词： acetylcholinesterase-1 Apolygus lucorum chlorpyrifos resistance inheritance

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Possibility of “Comet-Like” Transport of Acetylcholine Receptors in Command <i>Helix</i>Neurons in Cellular Analog of Habituation

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World Journal of Neuroscience 2014年第2期4卷 133-143页

作者： Arkady S. Pivovarov Galina B. Murzina Denis A. Makhnovsky Mariya S. Tret’yakova Department of Higher Nervous Activity Faculty of Biology Moscow Lomonosov State University Moscow Russia Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences Moscow Russia

The presence of "comet-like" radial transport of acetylcholine receptors by actin microfilaments without the participation of myosin motors in the depression of acetylcholine-induced inward chloric current (ACh-current) in command neurons of defensive behavior of the land snail, Helix lucorum, in a cellular analog of habituation was investigated. For that purpose the effects of CK548, CK-636 (inhibitors of actin-related protein complex Arp2/3, whose activation triggers rapid actin polymerization and the formation of the "comet-like" tail on the actinic filament) and wiskostatin (an N-WASP protein inhibitor, activating Arp2/3) on the depression of ACh-current were studied. The attenuation of ACh-current depression was observed upon the addition of CK548. At the same time, CK-636 and wiskostatin irreversibly strengthened the depression of this current and suppressed its spontaneous recovery. The results of CK-548 action and its mathematical modeling allow suggesting the presence of "comet-like" transport of acetylcholine receptors, initiated by Arp2/3 protein complex in receptor endo and exocytosis in command neurons of Helix lucorum in a cellular analog of habituation. Irreversible inhibition of vital metabolic processes of the neuron by wiskostatin and CK-636, which lead to the decrease in the level of ATP, could have caused irreversible effects of these blockers on current depression.

关键词： CK-548 CK-636 Wiskostatin Depression of Acetylcholine-Induced Inward Current Command Neurons Helix lucorum Cellular Analog of Habituation

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