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Effect of low-temperature treatment on bacterial cultivation in bacterial induced mineralization

Science China(Technological Sciences) 2022年第4期65卷 870-881页

作者： WANG Lu LIU ShuHua REN Jun State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan 430072China School of Architecture and Planning Yunnan UniversityKunming 650051China

In this paper,the effect of low-temperature treatment on bacterial activity was investigated and its performance in preparing the consolidated sand was *** factors,including absorbance(OD600),p H,electric conductivity(EC)and urease activity(UA)were monitored to evaluate the bacterial ***,the calcium carbonate yields under different bacterial solution and cementation solution volume ratios were ***,the compressive strength and microstructure of consolidated sand were investigated and the mechanism was explored by characterising the products among sand *** results showed that for both continuous and interval cultivation of bacteria,stable bacterial solution was harvested within the cultivation period of 30 ***-temperature treatment showed limited inhibition at early cultivation stage,while it had strong inhibition at middle cultivation *** interval cultivation improved the cultivation efficiency of *** was observed that the low-temperature preservation for a stable bacterial solution has little influence on UA before 4 days,while UA decreased seriously after its preservation time was extended to over 8 *** optimal volume ratio of bacterial solution and substrate solution was 1:1,resulting in the highest calcium carbonate *** improved compressive strength of consolidated sand after 3 injections was attributed to the irregular-shaped calcite products,which coated and bonded the sand grains together.

关键词： microbial mineralization cultivation methods low-temperature treatment bacterial activity microstructure

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