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Diversity of oligotrich ciliates(Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences

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Journal of Oceanology and Limnology 2020年第1期38卷 156-167,I0001-I0011页

作者： LU Kaihui LIU Weiwei Alan WARREN XU Yusen ZHU Changyu ZHAO Yan YI Zhenzhen Laboratory of Protozoology Guangzhou Key Laboratory of Subtropical Biodiversity and BiomonitoringSchool of Life ScienceSouth China Normal UniversityGuangzhou 510631China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Department of Life Sciences Natural History MuseumLondon SW75BDUnited K ingdom College of Life Sciences Capital Normal UniversityBeijing 100048China

Although the taxonomy of oligotrich ciliates has been widely investigated,yet the species diversity remains poorly *** newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 region,and it was effective for detecting oligotrich *** the primers,we constructed the cloning libraries to investigate the species diversity of oligotrichs in the northern coastal waters of the South China *** total,165 oligotrich sequences were obtained from five widely separated sampling *** operational taxonomic units(OTUs)were obtained at 99%similarity threshold,and low-abundance OTUs with no more than two sequences contributed most of these(about 78%).Our findings are consistent with previous morphological studies,Strombidium was found the most abundant and widely distributed genus in this *** addition,the BLAST search in the NCBI database resulted in 95%OTUs matching with named oligotrich species in similarity below 99%.Therefore,oligotrich morphospecies diversity has been underestimated as low-abundance species,and the LSU rDNA oligotrich sequence database needs to be better promoted.

关键词： diversity low-abundance species oligotrich ciliates operational taxonomic units (OTUs)

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Thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection

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Chinese Chemical Letters 2022年第1期33卷 334-338页

作者： Wei Zhang Liquan Liu Yangwei Liao Wan Shu Xiaofeng Tang Kejun Dong Zhihao Ming Xianjin Xiao Hongbo Wang Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Detection of point mutations in driver genes is of great significance for the early diagnosis,treatment,and prognostic evaluation of ***,current detection methods do not offer versatility,specificity,and rapid performance ***,multiple mutation detection processes are necessary,which results in long processing times and high *** this study,we developed a thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection(TTI-CS).This strategy is based on the DNA probe,which changes the thermodynamic balance of the DNA cascade by the designed bubble structure,thereby achieving a good distinction between mutant and wild-type *** designed method greatly shortens the detection time through two-way *** addition,this method only changes two inexpensive trigger and bridge sequences,which replace the specific and expensive nucleic acid probes used in analyses based on traditional DNA probe methods,thereby enabling multiple *** performed the detection of synthetic single-stranded DNA for the five mutation points and successfully detected in endometrial cancer *** detection limit of this method is0.1%,which better meets the needs of clinical low-abundance multiple mutation ***,TTI-CS is currently one of the best methods for detecting multiple mutation detections.

关键词： Nucleic acid probes DNA cascade system Universality low-abundance Multiple mutation detection

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