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The Impact of Varied Fertilizers on the Yield and Quality of Panicum miliaceum under low nitrogen Conditions

Phyton-International Journal of Experimental Botany 2024年第10期93卷 2629-2644页

作者： Mengyao Wang Jiao Mao Yuanmeng Xu Shu Wang Yuhan Liu Jiangling Ren Sichen Liu Zhijun Qiao Xiaoning Cao Center for Agricultural Genetic Resources Research Shanxi Agricultural UniversityTaiyuan030031China College of Agriculture Shanxi Agricultural UniversityJinzhong030801China Key Laboratory of Crop Genetic Resources and Germplasm Development in Loess Plateau Ministry of Agriculture and Rural AffairsTaiyuan030031China

To clarify the response characteristics of broomcorn millet yield and quality to various fertilizers under low nitrogen conditions,the present study investigated the effects of different fertilization treatments,including no fertilization(HCK,CCK),urea(HF1,CF1),phosphate fertilizer(HF2,CF2),compound fertilizer(HF3,CF3),and organic fertilizer(HF4,CF4),on the agronomic traits,quality,and starch pasting properties of proso *** experimental materials,non-waxy proso millet(Hequ red proso millet,denoted as HQH)and waxy proso millet(Chishu 1,denoted as CS1)were *** results showed that under low nitrogen conditions,urea treatment(HF1)significantly increased plant height and yield,and phosphate fertilizer treatment(HF2)significantly increased thousand-grain weight(TW).For waxy proso millet Chishu NO.1(CS1),urea treatment(CF1)significantly increased yield,phosphate fertilizer treatment(CF2)significantly influenced the height of the plants,and compound fertilizer treatment(CF3)significantly increased thousand-grain *** terms of quality,phosphate fertilizer treatment(CF2)and organic fertilizer treatment(CF4)resulted in a substantial augmentation of both the total starch content and amylose content of waxy proso millet Chishu NO.1(CS1),respectively,and organic fertilizer treatment(HF4,CF4)resulted in a notable enhancement of the protein content of proso *** fertilizer treatment(HF4)increased peak viscosity(PV),hot viscosity(HV),setback viscosity(SV),final viscosity(FV),breakdown viscosity(BV),pasting time(PTim),and pasting temperature(PTemp)of non-waxy proso *** fertilizer treatment(CF2)increased the peak viscosity(PV),hot viscosity(HV),breakdown viscosity(BV),final viscosity(FV),pasting time(PTim),and pasting temperature(PTemp)of waxy proso *** fertilizer treatment(HF4)and phosphate fertilizer treatment(CF2)under low nitrogen were the most effective in improving the comprehensive quality of non-waxy and waxy proso millet,r

关键词： Proso millet low nitrogen yield quality

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Cloning and Expression Analysis of Sucrose Non-fermenting-1 Related Protein Kinase (SnRK) in Cucumis sativus L. Under low nitrogen Conditions

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Journal of Northeast Agricultural University(English Edition) 2013年第4期20卷 1-9页

作者： He Hong-mei Qin Zhi-wei Feng Zhuo Wu Tao College of Horticulture Northeast Agricultural University

The sucrose non-fermenting-1 related protein kinase（SnRK）, whose expression is induced by kinds of hyperosmotic stresses, plays a key role in improving stress resistance of plants. In order to investigate the molecular mechanism of low nitrogen resistance in cucumber, the full-length cDNA of SnRK gene was cloned in this study. The result showed that SnRK gene was 1 548 bp in length, encoded 515 amino acids, and had more than 80% homology with other crops. The protein encoded by this gene was an unstable and hydrophilic protein with no transmembrane structure and no signal peptide. Under nitrogen-free conditions and low nitrogen conditions, the expression pattern analysis of SnRK gene showed that this gene was up-regulated and its expression increased and was significantly higher than the normal level as the nitrogen concentration decreased. In addition, the expression of SnRK gene was also inhibited in the high nitrogen level and was significantly lower than the normal level. The result of this study would help us understand the molecular mechanism of low nitrogen resistance in cucumber.

关键词： cucumber SnRK gene clone expression analysis low nitrogen

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Functional analysis of the nitrogen metabolism-related gene CsGS1 in cucumber

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Journal of Integrative Agriculture 2021年第6期20卷 1515-1524页

作者： XIN Ming QIN Zhi-wei YANG Jing ZHOU Xiu-yan WANG Lei College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030P.R.China College of Resources and Environment Northeast Agricultural UniversityHarbin 150030P.R.China

Glutamine synthetase(GS)plays an important role in nitrogen(N)metabolism in *** this study,we cloned and sequenced the CsGS1 gene,and analyzed the expression patterns and subcellular localization of the GS1 protein in response to different N conditions in order to determine its role in low-nitrogen(LN)***1 was abundantly expressed in the leaves of the low N-requiring cultivar D0328,while the high N-requiring cultivar D0422 showed similar expression levels across different tissues including leaves,shoots and ***,the GS1 protein was primarily localized in the cytoplasm of plant *** cultivars were then transformed with the CsGS1 coding sequence or antisense sequence via Agrobacterium tumefaciens in order to overexpress and silence GS1 expression,*** of CsGS1 significantly improved LN tolerance and photosynthetic parameters,and increased chlorophyll b content,biomass,plant height,root length,N accumulation and GS activity under LN condition compared to the ***1 silencing on the other hand significantly reduced the above *** together,CsGS1 is crucial for maintaining N metabolism in cucumber plants during N deprivation,and is a promising target for generating novel transgenic breeds with increasing nitrogen utilization efficiency.

关键词： cucumber CsGS1 low nitrogen functional verification

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