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Antiviral effects of hepatitis B virus S gene-specific anti-gene locked nucleic acid in transgenic mice

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World Journal of Clinical Cases 2018年第8期6卷 183-191页

作者： Shu-Rong Xiao Gui-Dan Xu Wu-Jun Wei Bin Peng Yi-Bin Deng Department of Medical Laboratory Science the Affiliated Hospital of Youjiang Medical College for Nationalities Department of Hepatobiliary Disease Center Guangxi Clinic Medicine Research

AIM To assess the antiviral effects of hepatitis B virus(HBV) S gene-specific anti-gene locked nucleic acid(LNA) in transgenic *** Thirty HBV transgenic mice were acclimatized to laboratory conditions and positive for serum HBV surface antigen(HBs Ag) and HBV DNA, were randomly divided into 5 groups(n = 7), including negative control(blank control, unrelated sequence control), positive control(lamivudine, anti-sense-LNA), and anti-gene-LNA experimental group. LNA was injected into transgenic mice by tail vein while lamivudine was administeredby gavage. Serum HBV DNA and HBs Ag levels were determined by fluorescence-based PCR and enzymelinked immune sorbent assay, respectively. HBV S gene expression amounts were assessed by reverse transcription polymerase chain reaction. Positive rates of HBsA g in liver cells were evaluated *** Average rate reductions of HBs Ag after treatment on the 3 rd, 5 th, and 7 th days were 32.34%, 45.96%, and 59.15%, respectively. The inhibitory effect of antigene-LNA on serum HBs Ag peaked on day 7, with statistically significant differences compared with pretreatment(0.96 ± 0.18 vs 2.35 ± 0.33, P < 0.05) and control values(P < 0.05 for all). Average reduction rates of HBV DNA on the 3 rd, 5 th, and 7 th days were 38.55%, 50.95%, and 62.26%, respectively. This inhibitory effect peaked on the 7 th day after treatment with anti-gene-LNA, with statistically significant differences compared with pre-treatment(4.17 ± 1.29 vs 11.05 ± 1.25, P < 0.05) and control values(P < 0.05 for all). The mR NA levels of the HBV S gene(P < 0.05 for all) and rates of HBsA g positive liver cells(P < 0.05 for all) were significantly reduced compared with the control groups. Liver and kidney function, and histology showed no abnormalities. CONCLUSION Anti-gene-LNA targeting the S gene of HBV displays strong inhibitory effects on HBV in transgenic mice, providing theoretical and experimental bases for gene therapy in HBV.

关键词： Anti-gene therapy Hepatitis B virus locked nucleic acid Hepatitis B Transgenic mice Anti-sensetherapy

STRESS AND DEFORMATION FIELDS ABOUT A locked FAULT IN DEPTH

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Science China Chemistry 1990年第6期33卷 735-746页

作者：杨修信陈沅俊 Institute of Crustal Dynamics State Seismological Bureau Beijing 100085 PRC

In this paper the stress and deformation fields about a locked fault in depth have been imitated using 3-dimensional finite element method and their time-space variation features have been analysed. The authors consider tentatively that the horizontal stresses about an active fault decrease slightly near the fault. The distributions of stress increasing and decreasing regions due to the locking of a fault are skew-symmetrical about the fault. The stresses about the locked region are increased in a larger range, where great elastic strain energy is accumulated and a stronger earthquake is more easily developed than at the ends of the fault. Reverse variations of crustal stress and deformation may be related to the different stages of the seismogeny of an earthquake focus.

关键词： locked fault in depth stress field deformation field.

locked and Loaded South Africa goes into coronavirus lockdown mode as the nation unites against a common enemy,despite economic uncertainty

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ChinAfrica 2020年第5期12卷 16-17页

作者： Tshawe Lama Tshawe 不详

South Africa goes into coronavirus lockdown mode as the nation unites against a common enemy,despite economic uncertainty The busy Central Avenue in the sprawling South African town of Kempton Park outside Johannesbur... 详细信息

关键词： locked coronavirus street

Surgical Treatment of Lower Cervical locked Facet. An Experience in 20 Cases

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Open Journal of Modern Neurosurgery 2019年第3期9卷 302-313页

作者： Wael A. Hammad Bokhary Mahmoud Al-Azhar University Cairo Egypt

Background: Cervical spine injuries are common as a result of a growing number of high energy accidents. The subaxial Injury Classification System and Severity Score (SLICS) suggest that a unilateral or bilateral facet dislocation must be managed surgically, even in the absence of SCI (Level of Evidence III). The surgical approaches could be anterior, posterior or combined anterior and posterior approaches. Methods: 20 patients, 12 males and 8 females, with age ranged from 20 to 45 years with lower cervical spine locked facet treated operatively by anterior or posterior spinal instrumentation after trial of closed reduction by skull traction. Results: In this series, satisfactory closed reduction was achieved in 15 patients with percentage 75% and fixed anteriorly, but 5 patients with percentage 25% needed posterior approach for reduction. Regarding ASIA score all of the patients improved at least one level after surgery except 2 cases with preoperative score A: they didn’t improve. Mean preoperative pain score VAS was 7.7 (range from 5 to 9) and postoperative mean of VAS was 0.75 with range from 0 to 3 until final follow up. Conclusion: Anterior approach with discectomy and fusion is necessary;however, if the closed reduction failed, posterior open reduction is indicated with lateral mass fixation, combined approaches can be indicated in specific cases such as increase of the kyphotic angle post anterior approach or when an osteotomy may be required to restore cervical alignment and neural decompression.

关键词： Cervical locked Facet Facet Dislocation Cervical Spine Injury

Multi-Order Intermittent Chaotic Synchronization of Closed Phase locked Loop

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International Journal of Modern Nonlinear Theory and Application 2018年第2期7卷 48-55页

作者： Samir M. Shariff Electrical Engineering Department College of Engineering Taibah University Medinah KSA

For the model of a Closed Phase locked Loop (CPLL) communication System consists of both the transmission and receiver ends. This model is considered to be in a multi-order intermittent chaotic state. The chaotic signals are then synchronized along side with our system. This chaotic synchronization will be demonstrated and furthermore, a modulation will be formed to examine the system if it will perfectly reconstruct or not. Finally we will demonstrate the synchronization conditions of the system.

关键词： Chaotic Synchronization Chaotic Signal Communication Systems Closed Phase locked Loop System Multi-Order Model

A Finite Element Model of locked Plating in Femoral Shaft Fractures

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Open Journal of Orthopedics 2014年第4期4卷 104-112页

作者： Brian E. Schwartz Farid M. L. Amirouche Kwang Won Choi Alfonso Mejia Mark Gonzalez Jacob R. Seiler College of Medicine University of Illinois at Chicago Chicago USA Department of Mechanical and Biomedical Engineering University of Illinois at Chicago Chicago USA Department of Orthopaedic Surgery University of Illinois at Chicago Chicago USA

Introduction: The Locking Compression Plate (LCP) system is a versatile technology that can be used either through conventional compression plating techniques or as an internal fixator with locking head screws. There have been only a few biomechanical studies examining the role of locked screw configuration on construct stability with most recommendations based upon empirical evidence or data from compression plating. This study will attempt to determine how different locked screw configurations, fracture gaps (distance between bone fragments), and interface gaps (distance between plate and bone) will affect the peak stress(von Mises stress) experienced by the plate-screw construct and, thereby, look at ways to minimize the risk of hardware failure. Materials Methods: A finite element model (FEM) was developed of a transverse mid shaft femoral fracture bridged by an eight-hole titanium LCP. Seven different screw configurations were investigated. Three different fracture gaps and three different interface gaps were studied as well. Results: The 1368 configuration was found to experience the least peak stress of 2.10 GPa while the 2367, 2457, and all filled configurations were found to have the highest peak stress (25.29 GPa, 22.78 GPa, and 23.54 GPa, respectively). Peak stress increased when the interface gap increased. Peak stress also increased as the fracture gap increased, with the largest jump between the 1 mm and 2 mm gaps. Conclusions: Every fracture is unique, and has a vast amount of parameters that must be considered when the surgeon is developing a treatment plan. For transverse femoral shaft fractures, the results of this study suggest that a working length of 2 screw holes on either side of the fracture may also lead to lower peak stress. In addition, our results demonstrate that minimizing the fracture gap and interface gap will lead to decreased stress in the plate-screw construct.

关键词： Finite Element Model locked Plating Femoral Shaft Fractures

Reconstruction of Humeral Head Defect in locked Posterior Dislocation Shoulder. A Case Series of Nine Patients

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Open Journal of Orthopedics 2015年第2期5卷 25-33页

作者： Said K. Abdel-Hameed Abdel-Khalek A. Ibrahem Alzalabany Mohamed A. Abdel-Aal Abdel-Aleem Soltan Orthopedic Surgery Faculty of Medicine for Girls Al-Azhar University Cairo Egypt Orthopedic Surgery El-Bakry General Hospital Ministry of Health Cairo Egypt

locked posterior dislocation shoulder is uncommon and frequently missed injury. It account for 2% - 4% of all shoulder dislocations. It is commonly associated with osseous defects in humeral head articular surface known as reverse Hill-Sachs lesion. Numerous surgical procedures invented to repair this defect with variable outcomes but evidence based management strategies are lacking. Among these procedures are: transfer of lesser tuberosity or subscapularis tendon, rotational osteotomy of humerus, osteochondral grafts. Salvage procedure as hemiarthroplasty or total shoulder arthroplasty used in huge non-constructable defect or very old neglected dislocation. In our case series, we treated 9 cases (2 females) of locked posterior shoulder dislocation with antero-medial humeral head defects ranging between 30% - 50% of head size. Open reduction of dislocation followed by transfer of the lesser tuberosity together with subscapularis tendon for reconstruction of the humeral head defect. The transfer was fixed with Ethibond suture size 5-0 (Ethicon, Inc. Somerville, New Jersy). The mean follow-up period was 14.5 months (range, 12 - 25 months). Seven cases had no pain or restriction of activities of daily living. No patient had symptoms of instability of the shoulder. According to UCLA Shoulder rating scale, there were 3 cases rated excellent, 4 cases rated good, one case rated fair and one case rated poor. It is concluded that reconstruction of the humeral head defect provides good pain relief, stability and function for patients with a locked posterior dislocation where the defect involves between 30% - 50% of the articular surface circumference. Our technique is simple, cheap and there is no need for second operation for hardware removal.

关键词： locked Posterior Shoulder Dislocation Reverse Hill-Sachs Lesion Anatomic Reconstruction

A Digital Phase locked Loop Speed Control of Three Phase Induction Motor Drive: Performances Analysis

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Energy and Power Engineering 2011年第1期3卷 61-68页

作者： Ben Hamed Mouna Sbita Lassaad 不详

This paper deals with performance analysis and implementation of a three phase inverter fed induction motor (IM) drive system. The closed loop control scheme of the drive utilizes the Digital Phase locked Loop (DPLL). The DPLL is safely implemented all around the well known integrated circuit DPLL 4046. An ex-perimental verification is carried out on one kw scalar controlled IM system drives for a wide range of speeds and loads appliance. This presents a simple and high performance solution for industrial applications.

关键词： Digital Phase locked Loop (DPLL) Induction Motor Scalar Strategy Speed Drives and Load Appliance

QUALITATIVE ANALYSIS OF THE PHASE locked LOOP OF THE THIRD ORDER WITH TANGENT DISCRIMINATOR CHARACTERISTIC

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Chinese Science Bulletin 1984年第1期29卷 6-9页

作者：王联王慕秋陆志奇 Institute of Mathematics Academia Sinica Beijing Xinxiang Teachers College Henan

As pointed out in [1], the technique of phase locking as a branch of newly developed technology has been widely applied in recent years to many fields such as radio communication, radar, aerospace sciences. A lot of practical and theoretical workers have engaged in the design and theoretical analysis of the second order phase locked loops. Up to the present, for the study of the third order loop, little is found

关键词： engaged aerospace pointed locking branch locked globally satisfying qualitative nowadays