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Ecotoxicological effects of mixed pollutants resulted from e-wastes recycling and bioaccumulation of polybrominated diphenyl ethers in Chinese loach(Misgurnus anguillicaudatus)

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Journal of Environmental Sciences 2009年第12期21卷 1695-1701页

作者： QIN Xiaofei XIA Xijuan LI Yan ZHAO Yaxian YANG Zhongzhi FU Shan TI-AN Mi ZHAO Xingru QIN Zhanfen XU Xiaobai YANG Yongjian State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences Beijing 100085 China Taizhou No.1 Middle School Taizhou 318000 China

To understand potential ecotoxicological effects of electrical and electronic equipment waste （e-waste） recycling and polybrominated diphenyl ethers （PBDEs） bioaccumulation in loaches, a semi-field experiment using Chinese loach （Misgurnus anguiUicaudatus） as experimental fish was performed. Larval loaches were kept in net-cage for three months in an e-wastes recycling site and a reference site in Southeastern China. There was significant difference of the survival rate between the loaches from the e-wastes recycling site （27%, 19/70） and from reference site （70%, 49/70）. Histopathological responses were also found in all the livers examined in loaches from the e-wastes recycling site. These results showed that mixed pollutants resulted from e-wastes recycling led to ecotoxicological effects on loaches. The bioaccumulation of polybrominated diphenyl ethers （PBDEs）, the main pollutants in e-waste, in loaches was also studied, the mean concentration of total PBDEs in sediment was 6726.17 ng/g wet weight and in water samples was 4.08 ng/L （dissolved phase）. BDE 209 was the dominant congener in sediment and with relatively high concentration in water. Relatively low concentration of BDE 209 （less than 0.01% of total PBDEs） and high concentration of BDE47 （up to 39.34% of total PBDEs） were detected in loaches.

关键词： e-wastes loach ecotoxicological effects polybrominated diphenyl ethers semi-field experiment

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A Novel Oligosaccharide from the Mucus of the loach

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Chinese Chemical Letters 2002年第4期13卷 321-324页

作者： ChuanGuangQIN KaiXunHUANG 等 DepartmentofBioengineering HubeiPolytechnicUniversityWuhan430068 DepartmentofChemistry HuazhongUniversityofScienceandTechnologyWuhan430074

A novel oligosaccharide was isolated and purified from the mucus of the loach, Misgurnus anguillicaudatus. It was identified by several qualitative tests and characterized by elementary analysis, UV and IR spectrum. Its average molecular weight (Mw=1539.4) was determined by gel permeation chromatography. The major structural monomers of Misgurnus anguillicaudatus oligosaccharide were identified to be D-galactose and L-fucose by paper chromatography and gas chromatography.

关键词： loach Misgurnus anguillicaudatus oligosaccharide isolation structural study.

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Development of Inter-Family Nuclear Transplant Embryos by Transplanting the Nuclei from the loach Blastulae into the Non-Enucleated Zebrafish Eggs

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Chinese Journal of Oceanology and Limnology 2003年第1期21卷 64-71页

作者：李荔张士璀袁金铎李红岩 Department of Marine Biology Ocean University of Qingdao Qingdao 266003 ChinaDepartment of Biotechnology Shenzhen University Department of Marine Biology Ocean University of Qingdao

The developmental fate of the pronuclei in recombined embryos obtained by transplanting the donor nuclei into the non-enucleated eggs remains controversial in the case of fish. In the present study, the nuclei from the loach blastulae were transplanted into non-enucleated zebrafish eggs, the resulting 9 inter-family nuclear transplant embryos developed to larval stages. Although the development timing of the nuclear transplants resembled that of zebrafish, chromosome examination revealed that most of the recombined embryos were diploids with karyotype characteristic of loach, which was also proved by RAPD analysis. Moreover, 3 out of the 9 larval fish formed barb rudiments specific to loach. It was therefore concluded that the nuclear transplant larval fish were inter-family nucleo-cytoplasmic hybrids; and that only the donor nuclei were involved in the development of the nuclear transplant embryos, while the pronuclei in the non-enucleated eggs were likely automatically eliminated during the development.

关键词： nuclear transplantation loach zebrafish nucleo-cytoplasmic hybrid

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Electrofusion between Blastula Cells and Unfertilized Egg in loach (Paramisgurnus dabryanus)

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Developmental and Reproductive Biology 1993年第2期2卷 51-61,T001-T003页

作者：李书鸿易泳兰陈宏溪 Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 Institute of Developmental Biology Chinese Academy of Sciences Beijing 100080

Electrofusion between blastula cells and unfertilized eggs in loach were investigated usingdielectrophoretic field where, under alternating sinusoidal electric field, blastula cells formed beads-like chain in close contact with the unfertilized egg and cell fusion occurred between eggs and thecells in tight contact with them. The nuclei ofblastula cells were brought into the cytoplasm of therecipient eggs, where they promoted the development of the fused eggs just like the zygote *** the development of the fused eggs was different from that of zygotes. Several nuclei might enterone and the same egg simultaneously and all of them could undergo division, resulting in severalblastomere after the first cleavage of the recipient egg. Before blastula stage, the embryo developingfrom the fused egg showcd irregular shape, but it was soon regulated and developed to a normalblastula which often continued its development into a normal individual. Cell/egg electrofusion cameto its highest fosion rate (80%) 8nd hatching rate (20%), with cell density at 1×10~3 cells/ml, Ca^(++)concentration at 10 mM, mannitol at 0.2 M and when the blastula cells were digested with 100μg/ml pronase E for 6-10 min at 20℃. The mechanism underlying development of electrofused eggsis discussed. As the result indicates, electrofusion might prove to be a promising biotechnology justas nuclear transplantation.

关键词： Blastula Cell Unfertilized Egg Electrofusion loach

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Infrared Spectroscopic Study of loach Liver under Cadmium Stress

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Agricultural Science & Technology 2008年第2期9卷 135-138页

作者：田志环德州学院生物系

[ Objective ] In order to study the influence of cadmium stress on loach liver. [ Method ] FTIR-NEXUSTM Fourier transform infrared spectrometer（FTIR） was used to scan liver sample with wavelength from 1 800 -400 cm^-1. The collected infrared spectrum was conducted deconvolution and curve fitting treatment to analyze changing trend of relevant macromolecular compounds in loach liver tissue under Cd^2＋ stress. [ Result ] The structures of protein and nucleic acid as well as their synthesis pathways changed significantly. [ Conclusion]FTIR technique possessed broad application value in studying animal resistance and environment monitoring.

关键词： FTIR Cadmium loach

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Study on the Toxicity of Acetochlor to loaches

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Agricultural Biotechnology 2020年第3期9卷 49-52,66页

作者： Yan WANG Peng DONG Pei XU Yuxiang LIN Zexin CHEN Yihong HU Chenzhong JIN Yiding WANG Kaifa GUO Qianxia ZHU Key Laboratory of Pesticide Hamless Application/Hunan Provincial Collaborative Innovation Center for Field Weeds Control Hunan University of HumanitiesScience and TechnologyLoudi 417000China Xinsheng Planting and Breeding Farmers Specialized Cooperative of Shuangfeng County Hunan ProvinceShuangfeng 417100China

[Objectives] This study was conducted to investigate the toxic effect of acetochlor in loaches. [Methods]loaches were put into the acetochlor solution with concentrations of 0,0. 062 5,0. 125,0. 25,0. 5 and 1 μl/L,respectively. The effects on dismutase( SOD),peroxidase( POD),catalase( CAT) and glutathione( GSH) activity and malondialdehyde( MDA) content in the liver of loaches were analyzed after 12,24,48 and 72 h of exposure. [Results] The activity of SOD,POD,CAT and GSH increased with the stress time and concentration,reached their maximums at 48 h after acetochlor treatment,and then ***,MDA content increased with the stress time and concentration. [Conclusions] Acetochlor had obvious oxidative stress on loaches,and the activity of SOD,POD,CAT and GSH showed an obvious induction-inhibition effect with the increase of acetochlor concentration and the extension of stress time.

关键词： Acetochlor loach Antioxidative enzyme system Malondialdehyde

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花鳅属鱼类一新种——斑条花鳅

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Current Zoology 1984年第1期 82-84页

作者：严纪平郑米良浙江水产学院

本文报道了作者于1982年5月、7月和11月采自浙江奉化溪口的鳅科花鳅属鱼类一新种,定名为斑条花鳅(Cobitis laterimaculata)。本文描述了该新种的形态特征,并简要记录其主要生态资料。还将该新种和近似种中华花鳅(Cobitis sinensis)就背... 详细信息

本文报道了作者于1982年5月、7月和11月采自浙江奉化溪口的鳅科花鳅属鱼类一新种,定名为斑条花鳅(Cobitis laterimaculata)。本文描述了该新种的形态特征,并简要记录其主要生态资料。还将该新种和近似种中华花鳅(Cobitis sinensis)就背鳍、臀鳍位置,脊椎骨数目,体色及个体大小等方面作了比较。

关键词： Pisces loach Cobitis laterimaculata sp nov. Upper reaches of Yongjiang River.

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Nuclear transfer of fish using delayed activating eggs as recipient

Nuclear transfer of fish using delayed activating eggs as re...

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2005水产科技论坛

作者： WU Yan, FU Hong-tuo , GONG Yong-sheng (Freshwater Fisheries Research Center, Key Laboratory of Genetic Breeding of Aquatic Animals and Aquaculture Biology, CAFS Wuxi 214081, China)

＜正＞The effects were assessed using the delayed activating eggs as recipient of nuclear transfer in comparison with normal recipient eggs. After 15 min and 30 min marinating of unfertilized eggs in artificial fertilization experiments, the average hatching rate of fertilized eggs of SOF group was 12. 6% and 5. 6% respectively, but that of control group only 2. 5% and 0. 15%. The fusion rate of SOF group was double that of control group averagely in nuclear transfer experiment, but the hatching rate was similar between them. Artificial fertilization experiments showed Synthetic Ovarian Fluid （SOF） certainly postponed the activation of unfertilized eggs and raised their hatching rate markedly. At the same time, results also indicated the activating state of recipient eggs was not the most important factor of nuclear transfer. Our work is significant theoretically to understand the relationship between activation and development of eggs as well as valuable to improvement of fusion parameters.

关键词： cell fusion nuclear transfer egg activation overmature fish loach

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Effect of New Fly Maggot Protein Feed on mRNA Expression of TOR Signaling Pathway-related Genes in loaches (Misgurnus anguillicaudatus)

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Agricultural Biotechnology 2021年第1期10卷 9-11页

作者： Haitao ZHANG Yunlong LI Yongliang CHEN Kailang GU Feng GAO Xuzhou Vocational College of Bioengineering Xuzhou 221006China

[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two kinds of test feed with equal nitrogen and energy were prepared by replacing 60% of the fish meal in the control group with the new fly maggot protein feed, i.e., Diet1(control group) and Diet2(60% fish meal replacement group). The feeding experiment was carried out in an indoor circulating water system, and the breeding period was 60 d. [Results] For the livers, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly higher than those in the Diet1 group(Ploach livers and muscles.

关键词： New fly maggot protein feed loach TOR signaling pathway

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