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In-situ synthesis of quantum dots in the nucleus of live cells

National Science Review 2024年第3期11卷 172-182页

作者： Yusi Hu Zhi-Gang Wang Haohao Fu Chuanzheng Zhou Wensheng Cai Xueguang Shao Shu-Lin Liu Dai-Wen Pang State Key Laboratory of Medicinal Chemical Biology Frontiers Science Centre for New Organic MatterTianjin Key Laboratory of Biosensing and Molecular Recognition Research Centre for Analytical Sciences College of Chemistry School of Medicine and Frontiers Science Centre for Cell Responses Nankai University Haihe Laboratory of Sustainable Chemical Transformations

The cell nucleus is the main site for the storage and replication of genetic material, and the synthesis of substances in the nucleus is rhythmic, regular and strictly regulated by physiological processes. However,whether exogenous substances, such as nanoparticles, can be synthesized in situ in the nucleus of live cells has not been reported. Here, we have achieved in-situ synthesis of CdSxSe1-xquantum dots（QDs） in the nucleus by regulation of the glutathione（GSH） metabolic pathway. High enrichment of GSH in the nucleus can be accomplished by the addition of GSH with the help of the Bcl-2 protein. Then, high-valence Se is reduced to low-valence Se by glutathione-reductase-catalyzed GSH, and interacts with the Cd precursor formed through Cd and thiol-rich proteins, eventually generating QDs in the nucleus. Our work contributes to a new understanding of the syntheses of substances in the cell nucleus and will pave the way for the development of advanced ‘supercells’.

关键词： glutathione live cell nucleus quantum dot intracellular synthesis

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Global mapping of live cell mechanical features using Peak Force QNM AFM

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Biophysics Reports 2020年第1期6卷 9-18页

作者： Jinrong Hu Shenbao Chen Dandan Huang Yan Zhang Shouqin Lü Mian Long Center of Biomechanics and Bioengineering Key Laboratory of Microgravity (National Microgravity Laboratory)Beijing Key Laboratory of Engineered Construction and Mechanobiology and CAS Center for Excellence in Complex System Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Atomic force microscopy(AFM) has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force mode.However, these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points,making the measurements time-consuming and highly localized. Peak Force Quantitative Nano Mechanics(PF QNM) is a high-speed(faster by 3–4 order of magnitude) and global surface mechanical property mapping method with high spatial resolution, overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity. In this protocol, we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials.

关键词： Global mechanical mapping live cell Atomic force microscopy PeakForce QNM

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3D Bioprinting with live cells

Engineered Regeneration

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Engineered Regeneration 2022年第3期3卷 292-309页

作者： Alicia Persaud Alexander Maus Lia Strait Donghui Zhu Department of Biomedical Engineering Stony Brook UniversityUSA

In recent years,the shortage of available organs for transplant patients has grown exponentially across the globe.Consequently,the healthcare industry is in dire need of artificial substitutes.Many recent research studies and tissue engineering groups have decided to utilize 3D bioprinting to produce these artificial organs.This synthetic organ printing is made possible by advancements in the materials required for the constructs,the printing method-ologies used to produce them,and the final physical structures’varying properties.The cutting-edge research and technology related to 3D and 4D live cell bioprinting have recently allowed researchers to produce multiple types of artificial organs and tissues.These tissues can be utilized for drug screening and organ replacement applica-tions.This article provides an extensive review of all the pertinent 3D live cell bioprinting technologies.First,we describe scaffolding methods and their comparison with the traditional technologies.Second,we explain the 3D bioprinting technology,its evolution,and its multiple types.Moreover,we describe the pros and cons of each bioprinting method.Third,we have discussed the critical bioink properties and their impact on the formation of 3D bioprinting models.In addition,we also describe the mechanical properties of bioprinters.Fourth,we have thoroughly discussed the various types of hydrogels and their properties.Every kind of hydrogel is utilized in specific applications,and we have presented a comprehensive list of its advantages and disadvantages.Fifth,we have discussed various applications of 3D bioprinting technology.We have considered a case study of human or-gans and elaborated on how bioprinters can revolutionize the organ replacement industry.Finally,we evaluated the possibility of 4D printing in the future organ industry,incorporating temporal factors into the bioprinting process.

关键词： Bioprinting Three-dimensional Scaffolding live cell Hydrogels 4D bioprinting Applications

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Super-resolution imaging of the dynamic cleavage of intercellular tunneling nanotubes

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Frontiers of Optoelectronics 2020年第4期13卷 318-326页

作者： Wanjun GONG Wenhui PAN Ying HE Meina HUANG Jianguo ZHANG Zhenyu GU Dan ZHANG Zhigang YANG Junie QU Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

As a new method of cell-cell communication,tunneling nanotubes(TNTs)play important roles in cell-cell signaling and mass exchanges.However,a lack of powerful tools to visualize dynamic TNTs with high temporal/spatial resolution restricts the exploration of their formation and cleavage,hindering the complete understanding of its mechanism.Herein,we present the first example of using stochastic optical reconstruction microscopy(STORM)to observe the tube-like structures of TNTs linking live cells with an easily prepared fluorescent dye.Because of this new imaging microscopy,the cleavage process of TNTs was observed with a high spatial resolution.

关键词： super-resolution tunneling nanotubes(TNTs) live cell

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Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells

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Biophysics Reports 2021年第5期7卷 377-383页

作者： Chenyi An Wei Chen Department of cell Biology and Department of Cardiology of the Second Affiliated Hospital Zhejiang University School of Medicine Zhejiang University Key Laboratory for Biomedical Engineering of Ministry of Education and State Key Laboratory for Modern Optical Instrumentation Zhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University

Complex physical cues including two-dimensional membrane environment, dynamic mechanical force,and bioelectric activity inevitably affect membrane receptor functions. Multiplexed single-molecule force spectroscopy(SMFS) techniques with the capability of live-cell measurements are essential to systemically dissect receptor’s functions under complex biophysical regulation. In this review, we summarize recent progress of live-cell based SMFS techniques and specifically focus on the progress of SMFS on the biomembrane force probe with enhanced mechanical stability and multiplexed capability of fluorescence imaging. We further suggest the necessity of developing multiplexed SMFS techniques with simultaneous bioelectric regulation capability to investigate membrane potential regulated membrane receptor functions. These state-of-art multiplexed SMFS techniques will dissect membrane receptors functions in a systematic biophysical angle, resolving the biochemical, biomechanical and bioelectrical regulatory mechanisms in physiologically relevant conditions.

关键词： Multiplexed SMFS techniques Membrane receptor Biophysical regulation live cell

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