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Genome-wide identification and characterization of the abiotic-stress-responsive lipoxygenase gene family in diploid woodland strawberry(Fragaria vesca)

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Journal of Integrative Agriculture 2022年第7期21卷 1982-1996页

作者： LI Zhi-qi XIE Qian YAN Jia-hui CHEN Jian-qing CHEN Qing-xi College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Horticultural Plant Biology and Metabolomics Center of Haixia Institute of Science and Technology Fujian Agriculture and Forestry UniversityFuzhou 350002P.R.China

lipoxygenase(LOXs)is a kind of dioxygenase without heme and iron,which plays an important role in the development and adaptation of many plants to the ***,the study of strawberry LOX gene family has not been *** this study,14 LOX genes were identified from the diploid woodland strawberry *** phylogenetic tree divides the FvLOX gene into two subfamilies:9-LOX and *** duplication event analysis showed that whole-genome duplication(WGD)/segmental duplication and dispersed duplication effectively promoted the expansion of strawberry LOX ***-PCR analysis showed that FvLOX genes were expressed in different *** profile analysis showed that FvLOX1 and FvLOX8 were up-regulated under low temperature stress,FvLOX3 and FvLOX7 were up-regulated under drought stress,FvLOX6 and FvLOX9 were up-regulated under salt stress,FvLOX2,FvLOX3 and FvLOX6 were up-regulated under salicylic acid(SA)treatment,FvLOX3,FvLOX11 and FvLOX14 were up-regulated under methyl jasmonate(MeJA)treatment,FvLOX4 and FvLOX14 were up-regulated under abscisic acid(ABA)*** analysis showed that FvLOX genes were involved in plant growth and development and stress *** analyzed and identified the whole genome of strawberry FvLOX family and characterized a variety of FvLOX candidate genes involved in abiotic stress *** study laid a theoretical and empirical foundation for the response mechanism of strawberry to abiotic stress.

关键词： lipoxygenase gene family strawberry abiotic stress expression pattern cis-acting element

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Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 443-450页

作者： Yu-ying ZHAO Chun-lu QIAN Ji-cheng CHEN Yan PENG Lin-chun MAO Department of Food Science and Nutrition College of Biosystem Engineering and Food Seience Zhejiang University Hangzhou 310029 China College of Horticulture and Gardening Yangtze University Jingzhou 434025 China

This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca^2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be the result of gene expression,which could be This study was to investigate the responses of phospholipase D（PLD） and lipoxygenase（LOX） to mechanical wounding in postharvest cucumber（Cucumis sativus ***-2） ***-associated Ca^2＋ content,activities and gene expression of PLD and LOX,and contents of phosphatidylcholine（PC）,phosphatidylinositol（PI）,and phosphatidic acid（PA） were determined in cucumber fruits following mechanical *** show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding,while membrane-associated Ca2＋ content *** with the increase of PLD and LOX activities,accumulation of PA and losses of PC and PI were observed in all fruits,but there were differences of degrees between wounded and control *** suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding *** activation of PLD and LOX might be

关键词： Cucumber Mechanical wounding Phospholipase D lipoxygenase Phospholipid

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Purification and characterization of lipoxygenase from Entermorpha clathrata

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Acta Oceanologica Sinica 2008年第1期27卷 92-101页

作者： YANG Wenge XUE Changhu XU Dalun FU Xueyan HE Xiong College of Food Science and Technology Ocean University of China Qingdao 266003 China Faculty of Life Science and Biotechnology Ningbo University Ningbo 315211 China Zhejiang Pharmaceutical College Ningbo 315100 China

The purification and characterization of lipoxygenase （LOX, EC 1.***.11.12） from the green algae Enteromorpha clathrata were studied. Two components marked LOX - 1 and LOX - 2 were purified and their molecular masses were estimated to be 102 and 79 ku by SDS - PAGE. Both LOX - 1 and LOX - 2 were stable over the pH wide range 6.0 - 10.0 and had the optimum pH of 10.0 and 8.6, at optimum temperature of 30 and 25℃, respectively. Substrate specificities of LOX - 1 and LOX - 2 were the greatest towards linoleic acid, followed by arachidonic acid and linolenic acid. The Michaelis constant values of LOX - 1 and LOX - 2 were 0.23 and O. 20 mmol/dm^3 with the substrate of linoleic acid. The LOX activities were stimulated greatly by Ca^2＋ but inhibited by Hg2 ~ and the antioxidants such as BHA, BHT and TBHQ. The hydroperoxide products of LOX were analysed by HPLC with the substrate of methyl linoleate, and the results showed that LOX - 1 formed mainly 9-hydroperoxides while LOX - 2 formed both 9- and 13-hydroperoxides at a ratio of 24： 76.

关键词： Entermorpha clathrata lipoxygenase purification characterization

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Induction of phenylalanine ammonia-lyase and lipoxygenase in cotton seedlings by mechanical wounding and aphid infestation

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Progress in Natural Science:Materials International 2005年第5期15卷 419-423页

作者： QIN Qiuju, SHI Xueyan, LIANG Pei and GAO Xiwu*(Department of Entomology, China Agriculture University, Beijing 100094, China) Department of Entomology China Agriculture University Beijing 100094 China

It has been suggested that infestation of plants causes increases in the activities of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX), key enzymes in the phenolic compounds synthesis pathway and the octadecanoid pathway, respectively. The purpose of this work is to investigate whether the infestation of cotton aphid (Aphis gossypii) and mechanical wound can cause the induction of PAL and LOX activities in cotton seedlings, and whether the induction occurs in healthy seedlings growing nearby the attacked ones. The specific activities of PAL and LOX were measured using spectrophotometric method after aphid infestation and mechanical wounding. Result indicated that PAL activity and LOX activity were greatly induced by mechanical wounding and aphid infestation in cotton seedlings. The induction of PAL and LOX occurred not only in wounded and infested seedlings but also in intact healthy seedlings growing nearby. After exposed to the aphid infestation-induced volatiles, the specific activity of PAL in cotton seedlings increased by 6% at 24 h, 80% at 48 h, 235% at 72 h compared to the control, and the specific activity of LOX increased by 18% at 24 h, 34% at 48 h, 24% at 72 h, respectively. In comparison, the specific activity of PAL in unwounded seedlings exposed to wound-induced volatiles increased by 0.0 at 24 h, 200% at 48 h, 164% at 72 h, respectively and the specific activity of LOX increased by 28% at 24 h, 37% at 48 h, 8% at 72 h, respectively. It suggests that the induced volatiles are involved in plant-plant communication as airborne transferred signals.

关键词： phenylalanine ammonia-lyase lipoxygenase cotton aphid mechanical damage cotton plants.

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Effect of Ethylene on Polygalacturonase,lipoxygenase and Expansin in Ripening of Tomato Fruits

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Transactions of Tianjin University 2009年第3期15卷 173-177页

作者：薛照辉寇晓虹罗云波朱本忠许文涛 School of Agriculture and Biological Engineering Tianjin University Zhongyi Animal and Science Technology Ltd. College of Food Science and Nutritional Engineering China Agricultural University

Fruit ripening is a complex process and is regulated by many factors. Ethylene and polygalacturonase （PG）, lipoxygenase （LOX）, expansin （EXP） are all critical regulating factors in fruit ripening and softening process. With antisense ACS tomato, Nr mutant tomato and cultivated tomato as materials, Northern blot hybridization showed that PG, LeEXP1 and LOXexpressed differently in different parts of cultivated tomato fruit during ripening, which was related to fruit ripening. The ripening process of columella and radial pericarp was faster than pericarp. In both Nr mutant and antisense ACS transgenic tomato fruit, expression levels ofPG, LeEXPI and LOXwere generally lower than those in cultivated fruit but still related to fruit ripening. The expression levels ofPG, LeEXP1 and LOX increased in the mature green tomato fruits after 0.5 h treatment with ethylene （100 μL/L）. These results indicate that gene expression ofPG, LeEXP1 and LOXwere positively regulated by ethylene. The time and cumulative effect of the concentration exists in the expression of PG regulated by ethylene. The regulation of LOX expression mainly depended on the fruit development after great amount of ethylene was produced. PG played a major role in ripening and softening of tomato fruit, and cooperated with the regulation of EXP and LOX.

关键词： tomato ripening and softening ethylene expansin lipoxygenase polygalacturonase gene expression

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12/15-lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy

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Journal of Diabetes Mellitus 2013年第3期3卷 101-110页

作者： Roman Stavniichuk Alexander A. Obrosov Viktor R. Drel Jerry L. Nadler Irina G. Obrosova Mark A. Yorek Department of Internal Medicine Eastern Virginia Medical School Norfolk USA Department of Veterans Affairs Iowa City Health Care System and Department of Internal Medicine University of Iowa Iowa City USA Pennington Biomedical Research Center Louisiana State University System Baton Rouge USA

Background: Increased mitogen-activated protein kinase (MAPK) phosphorylation has been detected in peripheral nerve of human subjects and animal models with diabetes as well as high-glucose exposed human Schwann cells, and have been implicated in diabetic peripheral neuropathy. In our recent studies, leukocytetype 12/15-lipoxygenase inhibition or gene deficiency alleviated large and small nerve fiber dysfunction, but not intraepidermal nerve fiber loss in streptozotocin-diabetic mice. Methods: To address a mechanism we evaluated the potential for pharmacological 12/15-lipoxygenase inhibition to counteract excessive MAPK phosphorylation in mouse and cell culture models of diabetic neuropathy. C57Bl6/J mice were made diabetic with streptozotocin and maintained with or without the 12/15-lipoxygenase inhibitor cinnamyl-3,4-dihydroxy-α-cyanocinnamate (CDC). Human Schwann cells were cultured in5.5 mMor30 mMglucose with or without CDC. Results: 12(S) HETE concentrations (ELISA), as well as 12/15-lipoxygenase expression and p38 MAPK, ERK, and SAPK/JNK phosphorylation (all by Western blot analysis) were increased in the peripheral nerve and spinal cord of diabetic mice as well as in high glucose-exposed human Schwann cells. CDC counteracted diabetes-induced increase in 12(S)HETE concentrations (a measure of 12/15-lipoxygenase activity), but not 12/15-lipoxygenase overexpression, in sciatic nerve and spinal cord. The inhibitor blunted excessive p38 MAPK and ERK, but not SAPK/ JNK, phosphorylation in sciatic nerve and high glucose exposed human Schwann cells, but did not affect MAPK, ERK, and SAPK/JNK phosphorylation in spinal cord. Conclusion: 12/15-lipoxygenase inhibition counteracts diabetes related MAPK phosphorylation in mouse and cell culture models of diabetic neuropathy and implies that 12/15-lipoxygenase inhibitors may be an effective treatment for diabetic peripheral neuropathy.

关键词： Diabetes lipoxygenase Neuropathy Schwann Cells Mitogen-Activated Protein Kinase

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Effects of 1-MCP treatment and cold storage on aroma and lipoxygenase genes expression in postharvest ‘Jinyan’ kiwifruit

Effects of 1-MCP treatment and cold storage on aroma and lip...

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第三届全国植物开花·衰老与采后生物学大会

作者： Xiaoxia An Yanan Zhang Miaolian Xiang Ming Chen Jinyin Chen College of Agronomy Jiangxi Agricultural University Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province Pingxiang University

In this study, the effects of 1μL/L 1-MCP treatment and cold storage（0±0.5℃） on aroma volatiles and lipoxygenase（LOX） genes expression in postharvest ’Jinyan’ kiwifruit were investigated, which aiming to provide theoretical basis and technical reference for postharvest storage and preservation of kiwifruit. The results showed that 1-MCP treatment and cold storage significantly inhibited the respiration rate, slowed the decrease of fruit firmness and the increase of total soluble solids（TSS） of ’Jinyan’ kiwifruit during storage. Furtherly, the relative content of aroma components were analyzed by GC-MS, which indicated that ethyl butyrate, hexanal and（E）-2-hexenal were the characteristic aroma volatiles in ’Jinyan’ kiwifruit. During the whole storage, ester volatiles content increased while aldehyde components decreased with the ripening of kiwifruit. Compared with the control, the production of ethyl butyrate was significantly inhibited by 1-MCP treatment and cold storage during shelf stage, whereas the concentration of hexanal and（E）-2-hexenal were higher in 1-MCP treatment and refrigerated fruits than the control. The genes expression analysis results showed that LOX gene family members had different expression pattern during kiwifruit ripening. The expression levels of AcLOX2, AcLOX3 and AcLOX4 increased rapidly at 2 d after 1-MCP treatment and higher than CK during the whole storage. But the transcript levels of Ac LOX1 and AcLOX5 were consistent with the changes of esters content with the prolongation of storage period, which indicated that AcLOX1 and AcLOX5 may be involved in the synthesis of esters in ’Jinyan’ kiwifruit.

关键词： Kiwifruit 1-MCP Aroma components lipoxygenase Cold storage

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In vitro antioxidant,antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae

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Journal of Integrative Medicine 2018年第4期16卷 255-262页

作者： Carola Analla Torres Cristina Marisel Perez Zamora Maria Beatriz Nunez Ana Maria Gonzalez Laboratory of Microbiology and Pharmaceutical Technology Department of Basic and Applied SciencesNational University of Chaco AustralPresidencia Roque Saenz Pena 3700ChacoArgentina National Council for Scientific and Technical Research(CONICET) Godoy Cruz 2290Buenos Aires C1425FQBArgentina Institute of Botany of the Northeast(IBONE-CONICET) Sargento Juan Bautista Cabral 2131CorrientesArgentina

Objectives： This study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. These species are Adenocalyrnrna rnarginatum （Cham.） DC., Amphilophium vauthieri DC., Cuspidaria convoluta （VEIL） A. H. Gentry, Dolichandra dentata （K. Schum.） L. G. Lohmann, Fridericia caudigera （S. Moore） L. G. Lohmann, Fridericia chica （Bonpl.） L. G. Lohmann and Tanaecium selloi （Spreng.） L. G. Lohmann. Methods： The antioxidant activity was evaluated using three methods, 2,2＇-azino-bis（3-ethylbenzothiazo line-6-sulfonic acid）（ABTS）, 2,2-diphenyl-l-picrylhydrazyl （DPPH）, and ferric reducing antioxidant power. lipoxygenase-inhibiting activity was assayed spectrophotometrically; the result was expressed as percent inhibition. The antimicrobial activity was assessed using the agar disk diffusion method. Minimal inhibitory concentration （MIC） and minimal bactericidal]fungicidal concentration were also determined for each extract against 12 pathogenic bacterial strains of Staphylococcus aureus and seven fungal strains of the Candida genus. The identification of the major compounds present in the most promising extract was established by high-performance liquid chromatography-tandem mass spectrometry. Results： C convoluta, F. caudigera, and F. chica exhibited the best antioxidant activity by scavenging DPPH and ABTS~ radicals and reducing Fe3＋ ion. These extracts showed a notable inhibition of lipnxygenase. F. caudigera was found to have the lower MIC value against S. aureus strains and six Candida species. The extracts of F. caudigera and C. convoluta were active even against methicillin-resistant S. aureus. C convoluta had higher total phenol content, better antioxidant activity and superior anti- inflammatory and antimicrobial activity. The main phenolic compounds found in this extract

关键词： Cuspidaria Fridericia Antioxidants lipoxygenase Antimicrobials Herbal drugs Free radical scavengers

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Development of Novel Assay for the Determination of lipoxygenases Activity

Development of Novel Assay for the Determination of Lipoxyge...

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第八届全国化学生物学学术会议

作者： Weiqiang Lu Xue Zhao Jin Huang Shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science and Technology

＜正＞lipoxygenases（LOXs） are important targets for inflammation-related *** the development of the anti-flammatory drug by targeting LOXs is slowed down because of the limit of existing assay *** the present study,we developed two new methods for the determintion of LOXs enzyme activity by taking advantage of the oxidation capacity of LOXs

关键词： lipoxygenase assay methods anti-inflammation durg

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From Vietnamese plants to a biflavonoid that relieves inflammation by triggering the lipid mediator class switch to resolution

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Acta Pharmaceutica Sinica B 2021年第6期11卷 1629-1647页

作者： Tran Thi Van Anh Alilou Mostafa Zhigang Rao Simona Pace Stefan Schwaiger Christian Kretzer Veronika Temml Carsten Giesel Paul M.Jordan Rossella Bilancia Christina Weinigel Silke Rummler Birgit Waltenberger Tran Hung Antonietta Rossi Hermann Stuppner Oliver Werz Andreas Koeberle Institute of Pharmacy/Pharmacognosy and Center for Molecular Biosciences Innsbruck(CMBI) University of InnsbruckInnsbruck 6020Austria Faculty of Pharmacy University of Medicine and Pharmacy at Ho Chi Minh City 700000Viet Nam Michael Popp Institute and Center for Molecular Biosciences Innsbruck(CMBI) University of InnsbruckInnsbruck 6020Austria Department of Pharmaceutical/Medicinal Chemistry Institute of PharmacyFriedrich Schiller University JenaJena 07743Germany Institute of Pharmacy Department of Pharmaceutical and Medicinal ChemistryParacelsus Medical University SalzburgSalzburgSalzburg 5020Austria Department of Pharmacy School of MedicineUniversity of Naples FedericoⅡNaples'80131Italy Institute of Transfusion Medicine University Hospital JenaJena 07747Germany

Chronic inflammation results from excessive pro-inflammatory signaling and the failure to resolve the inflammatory *** mediators orchestrate both the initiation and resolution of *** from pro-inflammatory to pro-resolving lipid mediator biosynthesis is considered as efficient strategy to relieve chronic inflammation,though drug candidates exhibiting such features are *** from a library of Vietnamese medical plant extracts,we identified isomers of the biflavanoid 8-methylsocotrin-4’-ol from Dracaena cambodiana,which limit inflammation by targeting 5-lipoxygenase and switching the lipid mediator profile from leukotrienes to specialized pro-resolving mediators(SPM).Elucidation of the absolute configurations of 8-methylsocotrin-4’-ol revealed the 2 S,γSisomer being most active,and molecular docking studies suggest that the compound binds to an allosteric site between the 5-lipoxygenase *** identified additional subordinate targets within lipid mediator biosynthesis,including microsomal prostaglandin E2 *** production is efficiently suppressed in activated human neutrophils,macrophages,and blood,while the induction of SPM biosynthesis is restricted to M2 *** shift from leukotrienes to SPM was also evident in mouse peritonitis in vivo and accompanied by a substantial decrease in immune cell *** summary,we disclose a promising drug candidate that combines potent 5-lipoxygenase inhibition with the favorable reprogramming of lipid mediator profiles.

关键词： lipoxygenase Inflammation Resolution Natural product Lipidomics Lipid mediator

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