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Relationship between enteric microecologic dysbiosis and bacterial translocation in acute necrotizing pancreatitis

World Journal of Gastroenterology 1998年第3期4卷 62-65页

作者： WU Cheng Tang, LI Zhan Liang and XIONG De Xin

AIM To investigate the potential role of intestinal microflora barrier in the pathogenesis of pancreatic infection. METHODS Fifteen dogs were colonized with a strain of *** JM109 bearing ampicillin resistance plasmid PUC18. The animals were divided into two groups. In experimental group ( n =8), acute necrotizing pancreatitis (ANP) was induced by injection of 0 5ml/kg of sodium tarocholate with 3000U/kg trypsin into the pancreatic duct. The control group ( n =7) underwent laparotomy only. All animals were sacrificed 7 days later. Mucosal and luminal microflora of intestine were analyzed quantitatively, and various organs were harvested for culturing, blood samples were obtained for determination of serum amylase activities and plasma lipopolysaccharide (LPS) concentrations. RESULTS In the experimental group, the number of *** in the intestine was much higher than those of the controls, while bifidobacterium and lactobacillus were decreased significantly (jejunum, 1 75±0 95 vs 2 35±0 79, P blood culture was 75 0% and 62 5% on day 1 and 2 after induction of ANP, respectively, but no bacterium was found in the controls. As compared with the control group, blood LPS levels and s

关键词： pancreatitis bacterial translocation intestines lipopolysaccharide/blood amylase/blood bifidobacterium lactobacillus

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Relationship between plasma D(-)-lactate and intestinal damage after severe injuries in rats

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World Journal of Gastroenterology 2001年第4期7卷 555-558页

作者： Xiao-Qing Sun Xiao-Bing Fu Rong-Zhan Yi Lü Qun Deng Xiao-Guo Jian Zhi-Yong Sheng Bum Institute, 304~(th)Hospital,Beijing 100037,China Department of General Surgery,Chinese PLA 304 Hospital,Beijing 100037,China Burn Institute Trauma Center 304th Hospital Beijing 100037 China. fu xb@***

AIM To explore the kinetic changes in plasma D(-)- lactate and lipopolyssccharide(LPS)levels,and investigate whether D(-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/ reperfusion,burn,and acute necrotizing pancreatitis (ANP). METHODS Three models were developed in rats:① gut ischemia/ reperfusion obtained by one hour of superior mesenteric artery occlusion followed by reperfusion;② severe burn injury created by 30% of total body surface area(TBSA)full-thickness scald burn;and ③ ANP induced by continuous inverse infusion of sodium taurocholate and trypsin into main pancreatic duct. Plasma levels of D(-)-lactate in systemic circulation and LPS in portal circulation were measured by enzymatic- spectrophotometric method and limulus amebocyte lysate (LAL)test kit,*** samples of intestine were taken for histological analysis. RESULTS One hour gut ischemia followed by reperfusion injuries resulted in a significant elevation in plasma D(-)- lactate and LPS levels,and there was a significant correlation between the plasma D(-)-lactate and LPS(r =0.719,Pblood paralleled with LPS levels in the portal vein *** measurement of plasma D(-)-lactate level may be a useful marker to assess the intestinal injury and to monitor an increase of intestinal permeability and endotoxemia following severe injuries in early stage.

关键词： gut/injury ischemia-reperfusion/ blood burn/blood acute necrotizing pancreatitis/blood D(-)-lactate/blood lipopolysaccharide/blood intestinal permeability

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