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Neural Regeneration Research 2014年第1期9卷 92-100页

作者： Xin Zhou Chun Yang Yufeng Liu Peng Li Huiying Yang Jingxing Dai Rongmei Qu Lin Yuan Department of Human Anatomy Histology and EmbryologySchool of Basic Medical SciencesSouthern Medical University

Amyloid-beta peptide is the main component of amyloid plaques, which are found in Alzhei- mer＇s disease. The generation and deposition of amyloid-beta is one of the crucial factors for the onset and progression of Alzheimer＇s disease. lipid rafts are glycolipid-rich liquid domains of the plasma membrane, where certain types of protein tend to aggregate and intercalate. lipid rafts are involved in the generation of amyloid-beta oligomers and the formation of amyloid-beta peptides. In this paper, we review the mechanism by which lipid rafts disturb the aberrant deg- radative autophagic-lysosomal pathway of amyloid-beta, which plays an important role in the pathological process of Alzheimer＇s disease. Moreover, we describe this mechanism from the view of the Two-system Theory of fasciology and thus, suggest that lipid rafts may be a new target of Alzheimer＇s disease treatment.

关键词： nerve regeneration lipid rafts amyloid precursor protein autophagy lysosome Alzhei-mer's disease Two-system Theory amyloid beta peptide autophagosome National Financial MajorProject of China neural regeneration

lipid rafts Identified on Synaptic Vesicles from Rat Brain

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Tsinghua Science and Technology 2006年第4期11卷 452-458页

作者：何立吕继华周庆华隋森芳 State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

For a long time, lipid rafts have been thought to participate in regulating neurotransmitter release. However, the existence of lipid rafts on synaptic vesicles （SVs） and the mechanism by which exocytosisrelative proteins distribute on this structure have not been fully investigated. There is also much controversial data concerning rafts on SVs and synaptic vesicle proteins which makes the results difficult to interpret. This study systematically analyzed the existence and properties of lipid rafts on purified SVs by sucrose density gradient centrifugation, cholesterol depletion, and temperature variation. The data reveals that typical lipid rafts on SVs are both cholesterol dependent and temperature sensitive. Previous confusing results may have been caused by improper treatment or side effects of particular reagent. We also screened the lateral distribution of major exocytosis-related SV proteins and found that only the synaptobrevin （syb） and synaptotagmin （syt） produce detectable association with lipid rafts in 1% Triton X-100.

关键词： synaptic vesicles lipid rafts cholesterol depletion synaptophysin synaptobrevin

Involvement of lipid rafts and Cellular Actin in AcMNPV GP64 Distribution and Virus Budding

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Virologica Sinica 2009年第4期24卷 333-349页

作者： F. J. Haines C. M. Griffiths R. D. Possee C. R. Hawes L. A. King School of Life Science Oxford Brookes University Veterinary Laboratories Agency-Wey-bridge New Haw Addlestone Surrey KT15 3NB UK. NERC Centre for Ecology & Hydrology (Oxford)

GP64 is the major envelope glycoprotein associated with the budded virus (BV) of Autographa californica nucleopolyhedrovirus (AcMNPV) and is essential for attachment and budding of BV particles. Confocal microscopy and flotation assays established the presence of lipid raft domains within the plasma membranes of AcMNPV-infected Sf9 cells and suggested the association of GP64 with lipid rafts during infection. GP64 and filamentous actin (F-actin) were found to co-localise at the cell cortex at 24 and 48 hpi and an additional restructuring of F-actin during infection was visualised, resulting in a strongly polarised distribution of both F-actin and GP64 at the cell cortex. Depletion of F-actin, achieved by treatment of Sf9 cells with latrunculin B (LB), resulted in the redistribution of GP64 with significant cytoplasmic aggregation and reduced presence at the plasma membrane. Treatment with LB also resulted in reduced production of BV in Sf9 cells. Analysis of virus gene transcription confirmed this reduction was not due to decreased trafficking of nucleocapsids to the nucleus or to decreased production of infectious progeny nucleocapsids. Reduced BV production due to a lack of GP64 at the plasma membrane of AcMNPV-infected Sf9 cells treated with LB, suggests a key role for F-actin in the egress of BV.

关键词： Autographa californica nucleopolyhedrovirus (AcMNPV) Actin lipid rafts Egress

Apatinib induces apoptosis of breast cancer cells by redistribution of Fas into lipid rafts

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Journal of Chinese Pharmaceutical Sciences 2019年第11期28卷 778-785页

作者： Shunchao Yan Xin Jiao Na Li Yangfan Du Chengyao Jiang Department of Oncology Shengjing Hospital of China Medical UniversityShenyang 110022China Department of Respiratory Medicine Shenyang Chest HospitalShenyang 110044China

Apatinib,an oral anti-angiogenic agent,has been shown to have anti-cancer effects for several cancer cell ***,little is known about the direct anti-tumor activity of apatinib for breast cancer ***,the direct effect of apatinib on breast cancer cells and its mechanism of action were *** viability was measured with a Cell Counting *** apoptosis was assessed by flow *** expression of caspase-8 and the cleavage of poly ADP ribose polymerase were assessed by Western blotting *** rafts and Fas distribution were determined by immunofluorescence *** suppressed breast cancer cell proliferation in a dose-dependent ***,apatinib enhanced the aggregation of lipid rafts and the redistribution of Fas into lipid *** with methyl-β-cyclodextrin,a cholesterol-sequestering agent,significantly reversed Fas redistribution and apatinib-induced *** conclusion,these results demonstrated that apatinib induced apoptosis of breast cancer cells partially through recruitment of Fas into lipid rafts.

关键词： Apatinib Breast cancer lipid rafts Fas

The Stability of lipid rafts-Like Micro-Domains Is Dependent on the Available Amount of Cholesterol

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Journal of Biophysical Chemistry 2016年第3期7卷 74-85页

作者： Thi Thuy Minh Nguyen Vasudeva R. Chintamsetti Sindhura Chennuru Department of Chemistry and Biochemistry Lamar University Beaumont TX USA

lipid rafts are sterol and sphingolipid rich membrane domains that possibly may play roles in multiple cellular processes. These domains are still the matter of debate and it is still unknown by which mechanism if any and organisms promote their formation. This study centers on the ease of in vitro formation of lipid rafts-like structures as it relates to the relative availability of sphingolipids, phospholipids, cholesterol, and membrane proteins. Following a 12 h incubation period, isolation and extraction of the lipid rafts-like assemblies, the composition of the structures was evaluated using HPLC. Cholesterol and sphingomyelin were detected at 206 nm and phosphatidylcholine was detected at 254 nm. Identification of lactose permease, a typical membrane protein, was done using FTIR. The thermal stability of the produced structures was also determined. Results show that the addition of cholesterol significantly increased both the amount of insoluble lipid rafts-like structures and their stability, and that the availability of a minimum amount of sphingolipid was necessary to produce larger amounts of more stable structures. However, the addition of phospholipids hindered the formation of lipid rafts-like assemblies and those formed were generally less stable.

关键词： lipid rafts Membrane Domains Cholesterol

Toy model that explains the regulation of cholesterol on lipid rafts

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Communications in Theoretical Physics 2022年第8期74卷 134-139页

作者： Dongyu Lyu Tanlin Wei Lei Zhang Yong Zhang School of Physics Sun Yat-sen UniversityGuangzhou 510275China

Cholesterol,as a common lipid on mammalian cell membranes,plays an important role in the formation of lipid *** experiments suggest that the strength of cholesterol's regulation on lipid rafts can be affected by the length of the unsaturated phospholipid acyl chain on the *** order to understand this observation,a simplified toy model containing three different molecules is proposed in this paper,where the tail length of phospholipids is *** model shows the regulation of membrane cholesterol on the phase separation of the lipid mixture and the formation of nano-domains,and also suggests that the configuration entropy of phospholipid tails is an essential factor.

关键词： cholesterol tail length of phospholipids lipid lattice model phase separation lipid rafts

Ethanol prevents damage of skin, kidney and joint in lupus-prone mice by regulating lipid rafts

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Ethanol prevents damage of skin, kidney and joint in lupus-p...

第十一届全国免疫学学术大会

作者： Dou Hui Liu Lena Qiao Wei Guo Xuanxuan Deng Guo-Min Nanjing Medical University Beth Israel Deaconess Medical Center

Objective:Ethanol has been elaborated to have a beneficial effect on destructive ***,the effect of ethanol on the development of systemic lupus erythematosus(SLE)remains *** study was performed to determine the potent... 详细信息

关键词： Systemic lupus erythematosus Ethanol organ tissue damage lipid rafts

Rta2p is required for the calcineurin-mediated resistance to fulconazole and the localization of proteins to lipid rafts in Candida albicans

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Rta2p is required for the calcineurin-mediated resistance to...

中国药理学会第十一次全国学术会议

作者： JIA Xin-ming~1,WANG Yu-jia~2,TANG Ren-jie~2,CAO Yong-bing~2,JIANG Yuan-ying~2 (1.Department of Immunology,Tongji University School of Medicine,Shanghai 200092,China 2.School of Pharmacy,Second Military Medical University,Shanghai 200433,China)

Candida albicans,the major opportunistic fungi of humans,causes life-threatening infections in immuno-compromised individuals such as AIDS patients,organ transplant patients,patients receiving parenteral nutrition,and cancer patients undergoing radiation *** to limited available therapy options and the emergence of drug resistance,treating fungal infections is challenging. To improve current treatment strategies,we have identified that Rta2p significantly reduced the sensitivity of fluconazole(FLC) to *** modulated by calcium signaling and the expression of RTA2 was under the control of the calcium-activated-calcineurin via its transcriptional factor Crz1p in *** the present study,we found genetically Rta2p was responsible for the reduced effect of calcium on the sensitivity of FLC to *** in a calcineurin dependent *** impairment of FLC to the plasma membrane of *** was calcineurin-dependently attenuated by ***,Rta2p was predominantly localized in lipid rafts,but not involved in its ***2p was required for the localization of 10 proteins to lipid *** these,5 proteins(Pma1p,Gas1p homologue,Erg11p,Pmt2p and Ali1p) have been reported to be raft-associated and Erg11p is a well-known target of azoles in ***. Rta2p was not involved in ***,genetically compromising the function of Rta2p significantly increased therapeutic efficacy of FLC against *** bloodstream ***-introduction of either one RTA2 allele or the fusion of oestradiol-inducible promoter and ORF of RTA2 significantly reduced FLC efficacy against ***-expression of RTA2 induced by oestradiol in vivo also significantly reduced FLC *** therapeutic potential of the influence of Rta2p on FLC efficacy was further demonstrated by in vivo studies that FLC therapy was significantly more efficacious when the function of Crz1p and Rta2p was alternatively and genetically *** conclus

关键词： Candida albicans azole resistance calcineurin pathway RTA2 lipid rafts

Activation of Integrin alpha5 Mediated By Flow Requires Its Translocation to Membrane lipid rafts in Vascular Endothelial Cells

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Activation of Integrin alpha5 Mediated By Flow Requires Its ...

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者： Xiaoli Sun Zhu Yi

Background-Local flow patterns determine the uneven distribution of atherosclerotic *** mechanical forces,membrane lipid rafts are crucial for shear stress-regulated endothelial *** investigate the role of lipid rafts in regulating endothelial function under shear stress,we compared the translocation of proteins in lipid rafts under different flow patterns by quantitative *** and Results-lipid raft proteins were isolated from endothelial cells（ECs）after 2-hr exposure to pulsatile shear stress（PS,12+/-4 dyn/cm2）or oscillatory shear stress（OS,0.5+/-4 dyn/cm2）.Among 396 proteins redistributed in lipid rafts,the level of integrin alpha5 was most significantly elevated in lipid rafts under ***,OS increased the level of activated integrin alpha5,which was exclusively located in lipid *** of F-actin-based cytoskeleton and knockdown of caveolin-1 prevented the OS-inducedintegrin alpha5 translocation and activation,suggesting significant roles of the cytoskeleton and caveolin-1 protein in integrin alpha5 ***,OS activation of integrin alpha5 induced the NLRP3 inflammasome,leading to increased inflammatory *** vivo,activation of integrin alpha5 and expression of adhesion molecules were increased in the atheroprone areas of LDLR-/-*** of integrin alpha5 with adenoviral short-hairpin RNA greatly attenuated EC dysfunction in partially ligated carotid arteries of the ***-Atheroprone flow activates integrin alpha5through lipid raft anchoring with ensuing induction of NLRP3 inflammasome in *** findings reveal a novel mechanotransduction mechanism by which disturbed flow causes endothelial dysfunction.

关键词： Integrin lipid rafts Shear Stress Proteomics Endothelial Dysfunction

Self-assembly on lipid rafts mechanically suppresses cancer cell migration

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Self-assembly on lipid rafts mechanically suppresses cancer ...

第十届全国化学生物学学术会议

作者： Guanying Li Ye Zhang Bioinspired Soft Matter Unit Okinawa Institute of Science and Technology

＜正＞lipid rafts play vital roles in cell mechanical stability that contributes to cancer metastasis rate,considered as promising membrane targets for cancer *** rafts show modified size,receptor composition,and link... 详细信息

关键词： Self-Assembly GP1-anchored PLAP lipid rafts Cytoskeleton Cancer Metastasis