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Single-cell RNA sequencing in cornea research:Insights into limbal stem cells and their niche regulation

World Journal of stem cells 2023年第5期15卷 466-475页

作者： Di Sun Wei-Yun Shi Sheng-Qian Dou State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of OphthalmologyEye Institute of Shandong First Medical UniversityQingdao 266000Shandong ProvinceChina Qingdao Eye Hospital of Shandong First Medical University Qingdao 266000Shandong ProvinceChina Eye Hospital of Shandong First Medical University Jinan 250000Shandong ProvinceChina School of Ophthalmology Shandong First Medical UniversityQingdao 266000Shandong ProvinceChina

The corneal epithelium is composed of stratified squamous epithelial cells on the outer surface of the eye,which acts as a protective barrier and is critical for clear and stable *** continuous renewal or wound healing depends on the proliferation and differentiation of limbal stem cells(LSCs),a cell population that resides at the limbus in a highly regulated *** of LSCs or their niche can cause limbal stem cell deficiency,a disease that is manifested by failed epithelial wound healing or even ***,compared to stem cells in other tissues,little is known about the LSCs and their *** the advent of single-cell RNA sequencing,our understanding of LSC characteristics and their microenvironment has grown *** this review,we summarized the current findings from single-cell studies in the field of cornea research and focused on important advancements driven by this technology,including the heterogeneity of the LSC population,novel LSC markers and regulation of the LSC niche,which will provide a reference for clinical issues such as corneal epithelial wound healing,ocular surface reconstruction and interventions for related diseases.

关键词： Cornea limbal stem cells Single cell RNA sequencing Heterogeneity Novel markers Niche regulation

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多孔复合水凝胶负载角膜缘干细胞重建眼表

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Science China Materials 2024年第7期67卷 2103-2114页

作者：王付燕许悦荷石德鹏程钧赵龙祁霞周庆军谢立信 Eye Institute of Shandong First Medical University State Key Laboratory Cultivation BaseShandong Provincial Key Laboratory of OphthalmologySchool of OphthalmologyShandong First Medical UniversityQingdao 266071China

干细胞疗法是一种用于重建角膜缘干细胞缺乏症(LSCD)患者眼表的新技术.然而,目前报道的干细胞疗法治疗LSCD仅局限于二维(2D)培养体系,不能模拟干细胞在体内生存的原生微环境.本研究基于多孔明胶甲基丙烯酰(GelMA)/丝素蛋白甲基丙烯酸缩... 详细信息

干细胞疗法是一种用于重建角膜缘干细胞缺乏症(LSCD)患者眼表的新技术.然而,目前报道的干细胞疗法治疗LSCD仅局限于二维(2D)培养体系,不能模拟干细胞在体内生存的原生微环境.本研究基于多孔明胶甲基丙烯酰(GelMA)/丝素蛋白甲基丙烯酸缩水甘油酯(SilMA),制备了一种可注射的光交联水凝胶用于负载角膜缘干细胞(LSCs)以重建眼表.多孔GelMA/SilMA水凝胶具有优异的透明度和良好的粘附性.更重要的是,该水凝胶保持了兔LSCs的活力,支持了兔LSCs的粘附,并促进了它们在体外3D培养环境中的迁移.蛋白质组学分析证实,多孔GelMA/SilMA水凝胶3D细胞培养体系能够保持LSCs的自我更新和分化能力.此外,组织学分析表明,负载LSCs的多孔GelMA/SilMA水凝胶能够有效的促进LSCD再上皮化.多孔水凝胶能够简化连续细胞培养的相关操作,以构建具有细胞均匀分布的眼表.这些发现为干细胞疗法和再生医学用于眼表重建提供了有价值的见解.

关键词： porous gelatin silk fibroin hydrogel limbal stem cells ocular surface reconstruction

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Ocular stem cells:a narrative review of current clinical trials

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International Journal of Ophthalmology(English edition) 2022年第9期15卷 1529-1537页

作者： Konstadinos Sotiropulos Dimitrios Kourkoutas Diamantis Almaliotis Katherine Ploumidou Vasileios Karampatakis Department of Ophthalmology 417 Army Shared Fund HospitalAthens 11521Greece Department of Ophthalmology 401 Army General Hospital of AthensAthens 11525Greece Laboratory of Experimental Ophthalmology School of MedicineAristotle University of ThessalonikiThessaloniki 54124Greece Department of Otolaryngology Children’s Hospital“Aglaia Kyriakou”Athens 11527Greece

·stem cells are undifferentiated cells showcasing a remarkable capacity of self-replenishing and differentiating into mature *** ability to proliferate connotes that a designated stem cell source is capable of generating an unrestricted number of mature *** ever-increasing comprehension of position,activity,and function of ocular stem cells has led to rapid progress and incessant improvement of possible procedures and therapies.A narrative review was conducted to summarize the current evidence on clinical trials and respective literature,regarding current evolution in the field of ocular regenerative *** tried to ascertain the safety of experimental and clinical procedures,their effectiveness,and the ethical repercussion of their use.

关键词： stem cells cell-based therapies limbal stem cells h ESC-derived retinal pigment epithelium human induced pluripotent stem cells olfactory mucosa mesenchymal stem cells

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