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Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice

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Cellular & Molecular Immunology 2018年第10期15卷 875-887页

作者： David Twapokera Mzinza Henrike Fleige Kristin Laarmann Stefanie Willenzon Jasmin Ristenpart Julia Spanier Gerd Sutter Ulrich Kalinke Peter Valentin-Weigand Reinhold Förster Institute of Immunology Hannover Medical SchoolHannoverGermany Institute for Microbiology University of Veterinary Medicine HannoverHannoverGermany Institute for Experimental Infection Research TWINCORECentre for Experimental and Clinical Infection Researcha joint venture between the Helmholtz-Centre for Infection Research and the Hannover Medical SchoolHannoverGermany Institute for Infectious Diseases and Zoonoses University of Munich LMUMunichGermany

Bronchus-associated lymphoid tissue(BALT)develops at unpredictable locations around lung bronchi following pulmonary *** formation and composition of BALT have primarily been investigated by immunohistology that,due to the size of the invested organ,is usually restricted to a limited number of histological *** assess the entire BALT of the lung,other approaches are urgently ***,we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the *** antibody staining of whole lung lobes and optical clearing by organic solvents,we present a method that allows in-depth visualization of the entire bronchial tree,the lymphatic vasculature and the immune cell composition of the induced ***,three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced *** this approach,we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8%of the entire lung volume in mice deficient in CCR7,in contrast to wild type mice(WT).Furthermore,BALT induced by heat-inactivated *** is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice,in contrast to WT mice.

关键词： Lung BALT light sheet microscopy E.coli MVA

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ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION

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Journal of Innovative Optical Health Sciences 2013年第1期6卷 1-15页

作者： L.SILVESTRI A.L.ALLEGRA MASCARO J.LOTTI L.SACCONI F.S.PAVONE European Laboratory for Nonlinear Spectroscopy(LENS)University of Florence Italy National Institute of Optics(INO-CNR) Sesto FiorentinoItaly Department of Physics University of FlorenceItaly International Center for Computational Neurophotonics-ICON Foundation FlorenceItaly

Understanding brain structure and function,and the complex relationships bet ween them,is one of the grand challenges of contemporary *** to their fexiblity,optical techniques could be the key to explore this complex *** this manuscript,we briefly review recent adv ancements in optical methods applied to three main issues:anatomy,plasticity and *** describe novel implement ations of light-sheet microscopy to resolve neuronal anat-omy in whole fixed brains with cellular *** to liv ing samples,we show how real-time dynamics of brain rewiring can be visualized through two-photon microscopy with the spatial resolution of single synaptic *** plasticity of the injured brain can also be dissected through cut ting edge optical methods that specifically ablate single neuronal ***,we report how nonlinear microscopy in combination with novel voltage sensitive dyes allow optical registrations of action potential across a population of neurons opening promising prospective in understanding brain *** knowledge acquired from these complementary optical methods may provide a deeper comprehension of the brain and of its unique features.

关键词： Brain optical microscopy light sheet microscopy two-photon microscopy

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