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Selenium distribution and nitrate metabolism in hydroponic lettuce(Lactuca sativa L.): Effects of selenium forms and light spectra

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Journal of Integrative Agriculture 2020年第1期19卷 133-144页

作者： BIAN Zhong-hua LEI Bo CHENG Rui-feng WANG Yu LI Tao YANG Qi-chang Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081P.R.China School of Animal Rural and Environmental SciencesNottingham Trent UniversityBrackenhurst CampusNG250QFUK

A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high content of nitrates, which poses a potential threat to human health. light is an important environmental factor that regulates the uptake and distribution of mineral elements and nitrogen metabolism in plants. However, the effects of Se forms and light conditions, especially light spectra, on the uptake and translocation of Se and on nitrate reduction are poorly understood. In this study, lettuce(Lactuca sativa L.) was treated with exogenous Se applied as selenate(10 mmol L^-1) and selenite(0.5 mmol L^-1) and grown under five different light spectra: fluorescent light(FL), monochromatic red LED light(R), monochromatic blue LED light(B), and mixed red and blue LED light with a red to blue light ratio at 4(R/B=4), 8(R/B=8), and 12(R/B=12), respectively. The effects of light spectra and Se forms on plant growth, photosynthetic performance, Se accumulation and nitrate reduction were investigated. The results showed that the light spectra and Se forms had significant interactions for plant growth, foliar Se accumulation and nitrate reduction. The Se concentration and nitrate content in the leaves were negatively correlated with the percentage of red light from the light sources. Compared to Se applied as selenite, exogenous Se applied as selenate was more effective in reducing nitrate via promoting nitrate reductase and glutamate synthase activities. The lowest nitrate content and highest plant biomass were observed under R/B=8 for both the selenate and selenite treatments. The significant effect of the light spectra on the root concentration factor and translocation factor of Se resulted in marked variations in the Se concentrations in the roots and leaves. Compared with FL, red and blue LED light led to significant decreases in the foliar Se concentration. The r

关键词： selenium light spectra nitrates nitrogen metabolism enzymes LEDs Lactuca sativa L.

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Transcriptome analysis reveals the differential regulatory effects of red and blue light on nitrate metabolism in pakchoi(Brassica campestris L.)

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Journal of Integrative Agriculture 2022年第4期21卷 1015-1027页

作者： FAN Xiao-xue BIAN Zhong-hua SONG Bo XU Hai Institute of Vegetable Crops/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Photobiology Research Center Institute of Urban AgricultureChinese Academy of Agricultural SciencesChengdu 610200P.R.China

Pakchoi(Brassica campestris L. ssp. chinensis) is an important leafy vegetable. Various light spectra, especially red and blue light, play vital roles in the regulation of nitrate metabolism. Information on the effects of red and blue light on nitrate metabolism at the transcriptome level in pakchoi is still limited, so this study used RNA sequencing technology to explore this molecular mechanism. Through pairwise comparisons with white LED light, 3 939 and 5 534 differentially expressed genes(DEGs) were identified under red and blue light, respectively. By Kyoto Encyclopedia of Genes and Genomes(KEGG) and Gene Ontology(GO) analyses, these unigenes were found to be involved in nitrate assimilation, plant-pathogen interaction, biosynthesis of secondary metabolites, and phenylpropanoid biosynthesis. The differential effects of light spectra on the nitrate concentration and metabolism-related enzyme activities were also confirmed at the physiological level. Several signal transduction modules, including Crys/Phys-COP1-HY5/HY5-like, were found to be involved in red and blue light-induced nitrate metabolism, and the transcript levels for this complex were consistent with the observed degree of nitrate assimilation. The expression patterns of 15 randomly selected DEGs were further validated using qPCR. Taken together, the results of this study could help improve our understanding of light spectrumregulated nitrate metabolism in pakchoi at the transcriptome level.

关键词： nitrate metabolism light spectra transcriptome gene expression pakchoi

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