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Chinese Medical Journal 2000年第3期000卷 36-39页

作者：傅建新陈子兴岑建农阮长耿

Objective To investigate the retrovirus mediated transfer and expression of multidrug resistance gene (mdr1) in hematopoietic cells and to develop a model for studying the possible reversal of the MDR mediated phenotype Methods A retroviral vector HaMDR expressing the human mdr1 gene was packaged by PA317 cells with a titer of up to 8 5×10 5CFU/ml K562 leukemia cells were infected with MDR retrovirus, and transfectant K562/MDR cells were generated The integration and expression of the exogenous mdr1 gene in K562/MDR cells were determined by polymerase chain reaction and flow cytometry The reversal ability of P glycoprotein (P gp) antagonists was analyzed by in vitro drug sensitivity, accumulation and efflux of rhodamine 123 (Rh123) in this model Results Transduction with amphotropic MDR retrovirus resulted in integration and expression of the mdr1 gene in the resistant cells, where an aberrant splicing transcript of the mdr1 gene was found The K562/MDR cells displayed a classic MDR phenotype with a 41-78 fold resistance to vincristine and colchicine in comparison with parental K562 cells The drug sensitivity of K562/MDR cells to vincristine can be completely restored by cyclosporin A (CsA, 2?mg/L) and Cremophor EL (CRE 132?mg/L), either individually or in combination ( P cells Conclusions Retroviral vector HaMDR allows transfection with high level expression of the mdr1 gene in human myeloid progenitor cells K562 The transfected K562/MDR provides a simple, sensitive model for developing antagonists of P gp and studying their mechanism of action

关键词： multidrug resistance gene leukemic cells P glycoprotein antagonists

Inhibition of Telomerase with hTERT Antisense Increases Susceptibility of leukemic cells to CDDP-induced Apoptosis

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The Chinese-German Journal of Clinical Oncology 2004年第1期3卷 42-46,67页

作者：张洹何冬梅广州暨南大学医学院血液病研究所 510632

Objective: To investigated the e?ect of inhibition of telomerase with hTERT antisense on leukemic cells (HL-60 and K562) to CDDP-induced apoptosis. Methods: Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and puri?ed. Telomerase activity was detected by Telomerase PCR ELASA kit and cell apoptosis was observed by morphological method and determined by ?owcytometry. Results: AS PS-ODN could signi?cantly inhibit telomerase activity by down regulat- ing the hTERT expression, and increase the susceptibility of leukemic cells to CDDP-induced apoptosis. Conclusion: Inhibition of telomerase with hTERT antisense can increases the susceptibility of leukemic cells to CDDP-induced apoptosis.

关键词： human telomerase reverse transcriptase Antisense phosphorothioate oligodeoxynucleotide telomerase leukemic cells cis-diamminedichloroplatinum

Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member B(ANP32B),Contributes to Retinoic Acid-induced Differentiation of leukemic cells

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Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member ...

Expressions of IL-10 on leukemic cells in acute leukemic patients and its significance

中国病理生理学会受体、肿瘤和免疫专业委员会联合学术会议

作者： Yun Yu,~1 Shaoming Shen,~2 Lishun Wang,~1 Guoqiang Chen~(1*) Department of Pathophysiology,Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Rui-Jin Hospital,Shanghai Jiao-Tong University School of Medicine

The acidic leucine-rich nuclear phosphoprotein 32B(ANP32B) is a member of a conserved superfamily of nuclear proteins that includes ANP32A/pp32,a factor that binds histones and inhibits their acetylation and regulates cell growth and differentiation in a tissue-specific *** this work,we provide the first demonstration that ANP32B expression is down-regulated during differentiation induction of leukemic cells by all-trans retinoic acid(ATRA).The ectopic expression of ANP32B partially inhibits ATRA-induced leukemic U937 cells differentiation,while knock-down of ANP32B expression by specific small interfering RNA enhances ATRA-induced differentiation to a degree,proposing the role of ANP32B in leukemic cell ***,ANP32B partially inhibits the transcriptional activity of retinoic acid receptor(RARα).Although the exact mechanism how ANP32B regulates the cellular event remains unresolved,our results suggest that ANP32B plays the antagonistic role regarding leukemic cells *** data will shed new insights into understanding the biological functions of ANP32B protein.

关键词： ANP32B differentiation leukemic cells

来源： cnki会议评论

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Journal of Nanjing Medical University 2005年第3期19卷 135-138页

作者： YunYang WanggangZhang YichaoQiao yinxiaChen XingmeiCAO DepartmentofHemotology theSecondHospitalofXi＇anJiaotongUniversityXi＇an710004China DepartmentofHemotology theSecondHospitalofXi＇anJiaotongUniversityXi＇an710004China

Objective: To explore the expressions of IL-10 on leukemic cells in acute leukemia patients and its significance. Methods: The expressions of IL-10 on leukemic cells in thirty patients was measured by indirect immunofluorescence technique. Results: Observed yellow-green bright fluorescence on leukemic cells membrane, the positive rate of cells was 10-80%, there were 18 patients expressing IL-10 (18/30, 60%) positively, among them 11 with ANLL (11/19, 58%) and 7 with ALL (7/11, 64%) respectively while that of peripheral mononucleate cells in control group was 13%. Compared with that in the control group, there was a significant increase of positive rate in ANLL and ALL but with no significant difference between ANLL and ALL. Conclusion: IL-10 secreted by leukemic cells, contributed to the immunosuppressive state at the tumor site. This is probably one of the important mechanisms of acute leukemic escape.

关键词： acute leukemia leukemic cells IL-10

Apoptosis of Leukemia cells Induced by CD34^+ cells Transferred Exogenous Fas Ligand

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第3期22卷 197-199页

作者：肖娟邹萍刘忠文胡中波刘凌波 Institute of Hematology Huazhong University of Science and Technology Xiehe Hospital Tongji Medical College Wuhan 430022 China

To assess the value of CD34 + cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34 + cells transfected with F asL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara C). After l8 h, apoptosis of cells was detected by FCM and TUNEL. Induced for l8 h by CD34 + cells transfected with FasL or without, the ratio of apoptos is of U937 cells was (5.0±1.3) %, (10.8±0.6) % ( P cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.

关键词： Fas ligand CD34 + cells leukemic cells apo ptosis

The effects of IL-10 on acute leukemic immune evasion

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Journal of Medical Colleges of PLA(China) 2005年第3期20卷 171-174页

作者：杨云张王刚曹星梅陈银霞刘捷田玮 Department of Hematology Xi'an Jiaotong University Second Hospital

Objective: To explore the effects of IL-10 on acute leukemic immune ***: Plasma concentrations of IL-10 were measured by ELISA in 56 first-visit acute leukemic *** expressions of IL-10 on leukemic cells in 30 patients were measured by indirect immunofluorescence technique. Results:Compared with those in control group,IL-10 concentrations increased significantly in first-visit acute leukemic *** there was a slight but not significant decrease of IL-10 in patients with acute lymphocytic leukemia(ALL) compared with those with acute non lymphocytic leukemic(ANLL).After intensive chemotherapy,there was a significant decrease of IL-10 in completely remitted(CR) patients,especially in those with ANLL,but there was still a significant increase compared with those in control *** positive rate of cells giving out yellow-green bright fluorescence on membranes was 10%-80%;there were 18 patients expressing IL-10(18/30,60%) positively:among them 11 with ANLL(11/19,58%) and 7 with ALL(7/11,64%) respectively while that of peripheral mononucleate cells in control group was 13%.Compared with that in control group,there was a significant increase of positive rate in ANLL and ALL but with no significant difference between ANLL and ***: Probably as one of important mechanisms of acute leukemic immune evasion,IL-10 secreted by leukemic cells,contributing to the immunosuppressive state at the tumor site,increase significantly in acute leukemic patients.

关键词： acute leukemia leukemic cells IL-10 immune evasion