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Universal probe with oriented antibody to improve the immunochromatographic assay of lead ions in Procambarus clarkii

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Food Quality and Safety 2023年第2期7卷 309-316页

作者：李杨阳朱正伟曲文莉杨庆刘言王桥段烁伍金娥宫智勇许琳 College of Food Science and Engineering Wuhan Polytechnic UniversityWuhanChina Hubei Provincial Institute for Food Supervision and Test WuhanChina Key Laboratory for Deep Processing of Major Grain and Oil Ministry of EducationWuhanChina Hubei Key Laboratory for Processing and Transformation of Agricultural Products WuhanChina Food Safety Research Center Key Research Instituteof Humanities and Social Sciences of Hubei ProvinceWuhanChina

Objectives:Based on the information from the random inspection of foods by the China Food and Drug Administration in 2022,the contamin-ation levels of lead ions are high in many edible *** methods of detecting lead ions cannot meet the requirements of on-site analysis of food due to the need for large *** immunochromatographic assay(iCA)is an effective,rapid,on-site analytical technique for determining lead ions in ***,the performance of ICA based on the traditional probe(AuNP-mAb)is limited by ignoring the influ-ence of theantibody *** and Methods:In this study,we developed an efficient technology for constructing a universal probe(AuNP-PrA-mAb)based on the oriented immobilization of *** performance of ICA was largely improved due to specific binding of the Fc region of the antibody with recombinant protein A(PrA)on the surface of a gold nanoparticle(AuNP).The ICA based on a universal probe was applied for the qualitative and quantitative detection of lead ions in Procambarus clarki within 30 ***,a simple and fast pretreatment method based on dilute acid extraction was developed forpretreating thePclarkii containing ***:The visual limit of detection and the scanning limit of quantization of the developed iCA strip for lead ions were O.5 ng/mL and 0.28 ng/mL,*** sensitivity of ICA based on universal probe was 10-fold higher than that of the ICA using traditional ***,the detection results had no obvious difference between the ICA and ICP-MS with t-test statistical ***:The developed ICA based on a universal probe presented broad application prospects in detecting contaminants in foods.

关键词： Oriented antibody universal probe immunochromatographic assay dilute acid extraction lead ions

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Synthesis of b-type Strontium Hydrogen Phosphate Nanosheets and Its Immobilization of Pb^(2+) in Acidic Aqueous Solution

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Acta Metallurgica Sinica(English Letters) 2015年第4期28卷 438-443页

作者： Fu-Qiang Zhuang Rui-Qin Tan Wen-Feng Shen Xian-Peng Zhang Wei Xu Wei-Jie Song Faculty of Electrical Engineering and Computer Science Ningbo University Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences

Highly crystalline β-type strontium hydrogen phosphate （β-SrHPO4） nanosheets were prepared by a hydro- thermal method and used for the immobilization of lead ions （Pb2＋） from acidic aqueous solution. The effects of various parameters on the immobilization process, including solution pH value, contact time, initial ion concentrations, and coexistent competing cations, were studied to optimize the conditions for maximum immobilization. The β-SrHPO4 nanosheets exhibited a capacity of （1,120 ± 22） mg/g toward Pb2＋ in acidic solution （pH value is 3.0）, and the equilibrium was achieved within 8 rain. The competing cations such as Cu2＋, Zn2＋, Cd2＋, and Co2＋ affected slightly on the selective immobilization of Pb2＋. The results revealed that the removal mechanism of Pb2＋ by the β-SrHPO4 nanosheets was the dissolution/precipitation process in the acidic solution.

关键词： Strontium hydrogen phosphate Nanosheets Immobilization lead ions Dissolution/precipitation

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Synthesis and adsorption performance of lead ion-imprinted micro-beads with combination of two functional monomers

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Journal of Environmental Sciences 2011年第12期23卷 1955-1961页

作者： Linyan Zhu Zhiliang Zhu Ronghua Zhang Jun Hong Yanling Qiu State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China Department of Chemistry Tongji University Shanghai 200092 China Key Laboratory of Yangtze River Water Environment Ministry of Education of China Shanghai 200092 China

A novel ion-imprinted polymer, lead ion-imprinted micro-beads with combination of two functional monomers, was synthesized using the W/O/W polymerization method. Two functional monomers, 1,12-dodecanediol-O,O’-diphenyl-phosphonic acid （DDDPA） and 4-vinylpyridine, were used to form a suitable construction with micro-pores fitting the template and recognition sites. The eflects of adsorbent dosage, solution pH and the competitive ions on the adsorption and separation eflciency of lead ions were investigated. The lead ion-imprinted micro-beads were eflcient for lead ions removal from aqueous solution in a broad pH range （4–9）, when the adsorbent dosage was above 0.1 g/L. The adsorption process obeyed the pseudo second-order kinetics model and it only took half an hour to reach the equilibrium. The adsorption isotherm of lead ion was described by the Langmuir model （R2 0.99） with a maximum adsorption capacity of 116.9 mg/g. In the presence of competitive ions Co2＋ and Cd2＋, the lead ion-imprinted micro-beads showed a high selectivity for lead ions. The selectivity coeficient of Pb2＋/Cd2＋ and Pb2＋/Co2＋are 99.3 and 114.7, respectively.

关键词： lead ions adsorption lead ion-imprinted micro-beads selective separation recognition

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lead removal from aqueous solution by employing natural brucite

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Journal of University of Science and Technology Beijing 2004年第5期11卷 394-397页

作者： LeiShao JiwenFu YueZhou JianfengChen ShouciLu ResearchCenteroftheMinistryofEducationforHighGravityEngineeringandTechnology CollegeofChemicalEngineeringBeijingUniversityofChemicalTechnologyBeijing100029China CivilandEnvironmentalEngineeringSchool UniversityofScienceandTechnologyBeijingBeijing100083China

The effect and mechanism of the removal of Pb^(2+) from an aqueous solutionby using brucite as the adsorbent were studied. It was revealed that the increase in pH of brucitesuspension, as a result of the release of magnesium hydroxide into the suspension, leads to a sharprise of the adsorption amount of Pb^(2+) on brucite. The synergism of buffering and adsorptioncapacities of brucite is responsible for the removal of Pb^(2+) from the aqueous solution. Thecoexistence of Cu^(2+) with Pb^(2+) causes a decrease of their adsorption on brucite due to acompetition for surface sites and brucite exhibits a higher adsorption capacity for Pb^(2+) than forCu^(2+). The percentage adsorption of Pb^(2+) on brucite could reach 96.38 percent, 97.20 percentand 94.09 percent respectively with the initial pH of the suspension pH_i = 1.76 (initial Pb^(2+)concentration [Pb^(2+)]_i = 20 mumol/L), 1.82 ([Pb^(2+)]_i = 100 mumol/L) and 1.84 ([Pb^(2+)]_i =500 mumol/L). It was concluded that brucite is a very efficient mineral adsorbent for Pb^(2+)removal from polluted acidic water.

关键词： lead ions brucite buffering adsorption removal

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Preparation of Chelating Polymer Grafted Magnetic Adsorbent and Its Application for Removal of Pb(Ⅱ) ions

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Journal of Wuhan University of Technology(Materials Science) 2011年第6期26卷 1108-1113页

作者：彭红黄开勋 School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

A novel magnetic chelating adsorbent （CPMS） with iminodiacetate functionality was prepared by polymerization of glycidyl methacrylate-iminodiacetic acid （GMA-IDA） monomer with N, N-methylenebisacrylamide as crosslinker in the presence of monodisperse magnetic silica microspheres （MS）. CPMS was characterized by IR, SEM, VSM and TGA. The experimental results revealed that MS was embedded in the gel polymer, but the morphology of CPMS was irregular. The saturation magnetization for CPMS was found to be 28.4 emu/g, and the percentage of GMA-IDA polymer grafted on MS was about 46.5%. CPMS were shown to be efficient for the removal of Pb（II） ions at pH 3.0 - 6.0, and the adsorption data obeyed the Langmuir equation with a maximum adsorption capacity of 54.4 mg？g？1 at pH 5.0. Moreover, the adsorption rate of CPMS was fast and it took about 5 minutes to achieve adsorption equilibrium in aqueous solution of lower lead ions concentration.

关键词： magnetic adsorbents chelating polymer grafted removal of lead ions

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Design of multiplex logic gates:combining regulation of DNA structure with logical calculation

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Science China Chemistry 2014年第3期57卷 453-458页

作者： TAO Jia ZHENG Jing LI JiShan ZHAO Peng LI JuanPing MA Cheng YI Mei YANG RongHua State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

In this paper,we proposed a facile and accurate way for controlling multiplex fluorescent logic gates through changing the exciting and the observing *** proof-of-principle,a Pb2+-specific DNAzyme probe and a thymine(T)-rich DNA probe were introduced to a double-stranded(ds-)*** addition style of the two ions served as the four inputs by changing the distance of the three fluorophores,6-carboxyfluorescein(FAM),ALEXA 532(ALEXA)and carboxytetramethylrhodamine(TAMRA),all of which were modified on the dsDNA *** with the previous methods,the present approach needed neither different inputs nor the change of sequence of the probe to achieve multiplex logic ***,the modularity of the strategy may allow it to be extended to other types of logic gates.

关键词： logic gates FRET DNA mercury ions(Ⅱ) lead ions

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Electrochemical sensor for Cd2+ and Pb2+ detection based on nano-porous pseudo carbon paste electrode

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Chinese Chemical Letters 2019年第12期30卷 2211-2215页

作者： Yuan Liu Taotao Li Chuxuan Ling Zhu Chen Yan Deng Nongyue He State Key Laboratory of Bioelectronics Southeast UniversityNanjing 210096China Hunan Key Laboratory of Biomedical Nanomaterials and Devices Hunan University of TechnologyZhuzhou 412007China Hunan Provincial Key Lab of Dark Tea and Jin-Hua School of Materials and Chemical EngineeringHunan City UniversityYiyang 413000China School of Chemistry and Chemical Engineering Southeast UniversityNanjing 211189China

An electrochemical sensor based on self-made nano-porous pseudo carbon paste electrode(nano-PPCPE)has been successfully developed,and used to detect Cd^2+ and Pb^2+.The experimental results showed that the electrochemical performance of nanoPPCPE is evidently better than both glassy carbon electrode(GCE)and pure carbon paste electrode(CPE).Then the prepared nano-PPCPE was applied to detect Cd^2+ and Pb^2+in standard solution,the results showed that the electrodes can quantitatively detect trace Cd^2+ and Pb^2+,which has great significance in electrochemical analysis and *** linear ranges between the target ions concentration and the D PASV current were from 0.1-3.0 μmol/L,0.05-4.0 μmol/L for Cd^2+ and Pb^2+,*** the detection limits were 0.0780 μmol/L and 0.0292 μmol/L,***,the preparation of the nano-PPCPE is cheap,simple and has important practical value.

关键词： Nano-porous pseudo carbon paste electrode (nano-PPCPE) Cadmium ions lead ions Sensor Detection

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