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NBS Profiling Identifies Potential Novel Locus from Solanum demissum That Confers Broad-Spectrum Resistance to Phytophthora infestans

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Journal of Integrative Agriculture 2014年第8期13卷 1662-1671页

作者： ZHANG Kun XU Jian-fei DUAN Shao-guang PANG Wan-fu BIAN Chun-song LIU Jie JIN Li-ping Institute of Vegetables and Flowers Chinese Academy of Agriculture Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop Ministry of Agriculture

Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates（race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11）. This coupled with nucleotide binding site（NBS） profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene（s）. Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region（SCAR） marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.

关键词： potato late blight resistance gene NBS profiling broa d-spectrum resistance

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Insect and Diseases Resistance in Tomato Entries

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American Journal of Plant Sciences 2021年第11期12卷 1646-1657页

作者： A. K. M. Quamruzzaman Ferdouse Islam Sharmilla Rani Mallick Olericulture Division Horticulture Research Center Bangladesh Agricultural Research Institute Gazipur Bangladesh

The study was conducted with 75 tomato entries at the farm of Olericulture Division, Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh during the winter season of 2020-21 to evaluate insect and disease reaction. Among the various insect and diseases of tomatoes, the late blight, TYLCV, bacterial wilt infection and leaf miner, fruit borer infestation are most common in Bangladesh. The TYLCV infection was observed 0% to 27% infection, while 47 entries showed zero percent infection. The range of bacterial wilt infection was 0% to 10% and zero percent infection was observed in 62 entries. In case of leaf miner infestation and fruit borer infestation, the range was 0% to 43% and 0% to 10%, respectively. Considering tolerance to late blight, TYLCV, bacterial wilt infection and leaf miner, fruit borer infestation, fruit size, fruit shape, plant growth nature, cluster nature of fruit, type of fruit ten entries AVTO 1010, AVTO 1706, AVTO 1713, AVTO 1829, AVTO 1909, AVTO 1911, AVTO 1915, AVTO 1921, AVTO 1954 and SLA 011 were found zero percent late blight, TYLCV, bacterial wilt infection and leaf miner, fruit borer infestation. So, these ten entries can be selected for disease and insect tolerant tomato varieties development as well as developing disease and insect tolerant hybrid tomato varieties.

关键词： Insect Diseases late blight TYLCV Bacterial Wilt Leaf Miner Fruit Borer Infestation Resistance Tomato

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Comparative Experiments of Potato Resistance to Potato late Bright in Zhaotong City

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Agricultural Science & Technology 2014年第4期15卷 561-563页

作者：马永翠杨艳丽石安宪雷崇义宋家雄昭通市植保植检站云南昭通657000 云南农业大学云南昆明650201 昭通农业学校云南昭通657000

A comparative test was conducted on 6 potato varieties in Songshan Vil- lage in Zhaoyang District during 3 years, and PB06, a new variety, was selected, which provides references for selecting highly-yielding, highly-... 详细信息

关键词： Potato late blight Tolerance Experiment

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