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中国神经科学学会第十二届全国学术会议

作者： Bing-bing Hu Min Chen Yu-bin Huang Rong-chen Huang Wu Yin Yang Xu Lei Li Bing Hu Chinese Academy of Sciences Key Laboratory of Brain Function and Disease Hefei National Laboratory for Physical Sciences at the Microscale School of Life SciencesUniversity of Science and Technology of China Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

Objective Zebrafish is an excellent model to study central nervous system（CNS） axonal degeneration and regeneration since we can observe these processes in vivo and in real time in transparent larvae. Previous studies have shown that Mauthner cell（M-cell） axon regenerates poorly after mechanical spinal cord injury. Inconsistent with this result, however, we have found that M-cell possesses a great capacity for axon regeneration after two-photon laser ablation. Methods By using ZEISS LSM 710 twophoton microscope, we performed specific unilateral axotomy of GFP labeled M-cells in the Tol-056 enhancer trap line larvae. Results Our results showed that distal axons almost degeneratedcompletelyat 24 hours after laser axotomy. After that, the proximal axons initiated a robust regeneration and many of the M-cell axons almost regenerated fully at 4 days post axotomy. Furthermore, we also visualized that regenerated axons wereremyelinated when we severed fluorescent dye labeled M-cells in the Tg（mbp:EGFP-CAAX） line larvae. Moreover, by single M-cell co-electroporation with Syp:EGFP and Ds Red2 plasmids we observed synapses reestablishment in vivo during laser injury-induced axon re-extension which suggested re-innervation of denervated pathways. In addition, we further demonstrated that microtubule destabilization with pharmacological treatment of nocodazole could completely abolish this regeneration capacity. Conclusion These results together suggested that in vivotime-lapse imaging of M-cell axon laser injury may provide a powerful analytical model for understanding the underlying cellular and molecular mechanisms of the CNS axon regeneration.

关键词： Mauthner cell laser axotomy degeneration regeneration remyelination synapse reestablishment microtubule

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Unraveling the mechanisms of synapse formation and axon regeneration: the awesome power of C. elegans genetics

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Science China(Life Sciences) 2015年第11期58卷 1084-1088页

作者： JIN YiShi Howard Hughes Medical Institute Section of Neurobiology Division of Biological Sciences University of California

Since Caenorhabditis elegans was chosen as a model organism by Sydney Brenner in 1960's, genetic studies in this organism have been instrumental in discovering the function of genes and in deciphering molecular signaling network. The small size of the organism and the simple nervous system enable the complete reconstruction of the first connectome. The stereotypic developmental program and the anatomical reproducibility of synaptic connections provide a blueprint to dissect the mechanisms underlying synapse formation. Recent technological innovation using laser surgery of single axons and in vivo imaging has also made C. elegans a new model for axon regeneration. Importantly, genes regulating synaptogenesis and axon regeneration are highly conserved in function across animal phyla. This mini-review will summarize the main approaches and the key findings in understanding the mechanisms underlying the development and maintenance of the nervous system. The impact of such findings underscores the awesome power of C. elegans genetics.

关键词： presynaptic active zone DLK kinase microtubule dynamics EFA-6 RPM-1 SYD-2 Liprin ubiquitin E3 ligase axoninjury laser axotomy

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