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laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis

Cell Research 1995年第1期5卷 9-24页

作者： GU GUOYAN (FORMERLY KU KUOYEN) CHENGTANG XU KONGHUA ZHANG QIRONG GAO.(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China) Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.&#x0002A Dedicated to Prefessor Zhen YAO on the occasion of his 80th birthday.† This work was supported by the director's foundation of Shanghai Institute of Cell BiologyReceived 27October1994 Revised 6April1995 Accepted 25April1995

By laser scanning fluorescence microscopy for quan-titative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocyanate duxing cleavage furrow extending forward, it was found that in area of presumptive cleavage furrow the scanning curve became ∨ shape, indicating dark stripe appeared in that place. Then the fluorescence intensity increased at the place where the botton of ∨ shape had located, and the scanning curve tuxned to ∧ shape, indicating single stripe was formed. While enhanced fluorescence appeared on the borders of ∧ shape, an M shape curve was found, show-ing double stripe occurred. During the distance between two borders of M shape incresing from 50 μm to 100μm,a fluorescence peak came to sight in the middle of the M shape, which being the cleavge furrow bottom. The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane. At that time the curve became W shape. By the sides of cleavage furrow the the stress folds became conspicous after double stripe stage, showing the stretching of the egg surface being increased. With our[31, 33] and others[32] reports that polylysine could induce the appearance of nascent membrane and phyto-hemagglutinins could decrease or prevent the appearance of nascent membrane, we believed the idea of Schroeder[25] that increasing mechanical stress could initiate nascent membrane formation and thought that the stress lay to the outsides of cleavage furrow.

关键词： cleavage egg surface movement initiating nascent membrane formation laser scanning fluorescence microscopy Rana Amurensis

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