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Asian Pacific Journal of Tropical Biomedicine 2018年第4期8卷 217-225页

作者： Aksorn Chantawee Mayura Soonwera Department of Plant Production Technology Faculty of Agricultural Technology King Mongkut's Institute of Technology Ladkrabang

Objective:To evaluate larvicidal,pupicidal and oviposition deterrent activities of four plant essential oils from Alpinia galanga(L.)Willd rhizome,Anethum graveolens L.(***)fruit,Foeniculum vulgare ***,and Pimpinella anisum *** against Aedes aegypti(***).Methods:Four essential oils at 1%,5%and 10%concentrations were assessed for insecticidal activity against larvae and pupae of ***,following the procedure of a dipping method *** deterrent activity of four essential oils was evaluated on gravid female of *** by a dual-choice oviposition ***:The results revealed that *** oil provided the strongest larvicidal activity against *** among four tested plant essential oils with the highest mortality rate of 100%and LC_(50)value of-0.3%.From the pupicidal experiment,*** also showed the highest toxicity against *** pupae with the highest mortality rate of 100%at 72 h and LC_(50)value of2.9%.In addition,10%*** had an oviposition deterrent effect against *** with effective repellency of 100%and an oviposition activity index ***:*** oil has a good potential as a larvicidal,pupicidal and oviposition deterrent agent for controlling ***.

关键词： Aedes aegypti Plant essential oils larvicide Pupicide Oviposition deterrent

Wild chive oil is an extremely effective larvicide against malaria mosquito vector Anopheles stephensi

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Asian Pacific Journal of Tropical Medicine 2019年第4期12卷 170-174页

作者： Alireza Sanei-Dehkordi Moussa Soleimani-Ahmadi Yaser Salim Abadi Azim Paksa Department of Medical Entomology and Vector Control School of Health Hormozgan University of Medical Sciences Infectious and Tropical Diseases Research Center Hormozgan Health Institute Hormozgan University of Medical Sciences Department of Health Services and Health Promotion School of Health Rafsanjan University of Medical Sciences Tabriz University of Medical Sciences

Objective: To assess the chemical composition and mosquito larvicidal potentials of essential oil of wild chive(Allium schoenoprasum L.) against Anopheles ***: In the search for an environmentally safer alternative mosquitoes control, the larvicidal efficacy of essential oil obtained from the leaves of Allium schoenoprasum L. against Anopheles stephensi was determined. The composition of chive essential oil was analyzed by gas chromatography–mass spectrometry. Results: In toxicity assays, the essential oil demonstrated substantial larvicidal activity against larvae of Anopheles stephensi with LC_(50) and LC_(90) values of 2.60, and 7.31 ppm after 24 h of exposure, respectively. Gas chromatography–mass spectrometry analysis of chive essential oil identified 35 components representing more than 97.31% of the total essential oil. The main constituents were sulfur compounds, including diallyl trisulfide(13.72%), 2-ethyl[1,3]dithiane(8.93%), allyl methyl trisulfide(8.77%), and trimethylene trisulfide(6.64%), respectively. Conclusions: This study demonstrated that wild chive essential oil has a rich source of eco-friendly bioactive compounds for use as a mosquito larvicide. The main reason for its extraordinary properties may be related to the high percentage of sulfur compounds.

关键词： larvicide Allium schoenoprasum Chive Essential oil Gas chromatography–mass spectrometry Anopheles stephensi

Bioactivity guided isolation of mosquito larvicide from Piper longum

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Asian Pacific Journal of Tropical Medicine 2011年第2期4卷 112-116页

作者： Madhu.S Kumar Vijayan VA Shaukath AK Vector Biology Research Lab Department of Studies in ZoologyUniversity of MysoreManasagangotriMysore 570 006KarnatakaIndia Department of Studies in Zoology University of MysoreManasagangotriMysore 570 006KarnatakaIndia Department of Chemistry Yuvaraja's CollegeUniversity of MysoreMysore-05India

Objective:To isolate the larvicidal component from the fruits of Piper longum（***） against the filariasis vector,Culex quinquefasciatus（***）.Methods:Pulverized fruits of *** were subjected to soxhlet extraction using series of organic solvents of increasing *** the solvent extracts were verified for their larvicidal efficacy against 4th instar larvae of *** employing standard WHO *** fractionation through column chromatography lead to the isolation of a bioactive amide, pipyahyine from the petroleum ether ***:Petroleum ether extract was found to be the most active fraction among all the extracts tested with LC50 and LC90 being 1.03 and 2.04 ppm ***,pipyahyine,an isolated component of the same fraction was found to be even more effective than the parent extract in terms of LC50 being 0.58 and 1.88 ppm respectively. Conclusions:From the results,it is evident that *** can be considered as a powerful arsenal for the control of mosquito population.

关键词： Piper longum Bioassay Pipyahyine Culex quinquefasciatus Filariasis larvicide

Mosquito larvicidal activity of some common spices and vegetable waste on Culex quinquefasciatus and Anopheles stephensi

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Asian Pacific Journal of Tropical Medicine 2011年第4期4卷 288-293页

作者： Someshwar Singha Goutam Chandra Mosquito and Microbiology Research Units Parasitology LaboratoryDepartment of ZoologyBurdwan University

Objective:To investigate the larvicidal activities of crude and chlor of orm:methanol(1:1 v/ v) extracts of some common spices(Cuminum cyminum,Allium sativum,Zingiber offinale, Curcuma longa) and vegetable waste(Solanum tuberosum germinated tuber) against Anopheles stephensi and Culex quinquefasciatus mosquito ***:Larval mortality of above mosquito species were observed after 24,48 and 72 h of exposure to five concentrations of aqueous extract(0.1%,0.2%,0.3%,0.4%and 0.5%) and four concentrations(25,50,75 ppm) of chlor of orm:methanol(1:1 v/v) *** lethal concentration of individual spices or vegetable waste was determined by log-probit analysis(at 95%confidence level) and effect of crude and chlor of orm:methanol(1:1 v/v) extracts were recorded on non target ***: Relative mortality rate of both larval mosquito species were recorded in the following sequences: Cuminum cyminum> Allium sativum>Zingiber offinale,Curcuma longa > Solanum tuberosum germinated tuber for crude extract,and efficacy of chlor of orm:methanol(1:1 v/v) extract were as follows:Curcuma longa > Zingiber offinale > Solanum tuberosum germinated tuber > Cuminum cyminum> Allium ***:Crude and chlor of orm:methanol(1:1 v/v) extract of Cuminum cyminum,Allium sativum,Zingiber offinale,Curcuma longa and Solanum tuberosum germinated tuber can be recommended effectively in mosquito control programmes at very low *** mortality and other abnormalities were noticed on non target organisms and further studies are needed to investigate the chemical structure of active principal which are responsible for larvicidal activity.

关键词： Mosquito larvicide Cuminum cyminum Allium sativum Zingiber offinale Curcuma longa Solanum tuberosum germinated tuber Anopheles stephensi Culex quinquefasciatus

Susceptibility of Aedes albopictus from dengue outbreak areas to temephos and Bacillus thuringiensis ***

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 295-300页

作者： Ahmad Mohiddin Asmalia Md Lasim Wan Fatma Zuharah School of Biological Science Universiti Sains Malaysia Centre of Chemical Biology Universiti Sains Malaysia Vector Control Research Unit School of Biological ScienceUniversiti Sains Malaysia

Objective:To monitor the current duration of the application rates in vector programme and the level of Aedes albopictus larvae susceptibility from three selected areas in northeast district of Penang on two selected larvicides,temephos and Bacillus thuringiensis ***(Bti) which are commonly used by Penang Health Department for vector ***:The mosquito larvae were tested against two types of larvicides:(1) temephos(Abate®) with diagnostic dosage(0.012 mg/L) and operational dosage(1 mg/L) and(2)Bacillus thuringiensis ***(Vecto Bac®WG) with operational dosage ranging from 6 000 international toxic units per L to 24 000 international toxic unit per L.A total of 20 late third and early forth instar larvae were selected and transferred into paper cup sized 300 m L using wide-mouthed *** larvae were distributed into each 300 m L paper cup containing 50 m L of aged tap *** experiment was replicated five times for each concentration *** test was repeated three *** mortality was recorded after 24 h of exposure and recorded lethal time was based on 2 h for temephos and 6 h for *** control consisted of ethanol for temephos and only seasoned water for ***:The result showed that Aedes albopictus from Flat Hamna,Kampung Sungai Gelugor and Kampung Tanjung Tokong were still susceptible to Bti and ***,higher lethal time and resistance ratio were detected in strain from Flat Hamna which was a known dengue hot spot area in northeast of ***:The application of temephos and Bti in vector control activity in these selected localities is still relevant in the control of Aedes larvae populations.

关键词： Aedes albopictus Mosquito larvicide Susceptibility

Adulticidal, larvicidal, pupicidal and oviposition deterrent activities of essential oil from Zanthoxylum limonella Alston(Rutaceae) against Aedes aegypti(L.) and Culex quinquefasciatus(Say)

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Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 967-978页

作者： Mayura Soonwera Siriporn Phasomkusolsil Department of Plant Production Technology Faculty of Agricultural TechnologyKing Mongkut's Institute of Technology LadkrabangBangkok 10520Thailand Department of Entomology Armed Forces Research Institute of Medical SciencesBangkok 10400Thailand

Objective: To evaluate adulticidal, larvicidal and oviposition deterrent response of the essential oil from dried Zanthoxylum limonella(Z. limonella) fruit against Aedes aegypti(Ae. aegypti) and Culex quinquefasciatus(Cx. quinquefasciatus).Methods: Z. limonella oil was tested by biological assays at 1%, 5% and 10% concentrations in ethanol. Adulticidal efficacy was tested against the 2–3 day old adult females. Larvicidal activity was tested against immature stage of mosquitoes. Oviposition deterrence of the oil was evaluated on gravid ***: The adult mortality was observed after 24 h with the LC50 of 6.0% for ***, and 5.7% for Cx. quinquefasciatus. Larvicidal bioassay was carried out with the 10% Z. limonella oil against immature stages of Ae. aegypti and Cx. quinquefasciatus,which caused 100% mortality after 12 h and 24 h. In the larvicidal experiment,Z. limonella showed effective result at 1%, 5% and 10% concentrations with the values of LT50 Ae. aegypti = 9.78, 5.61, 0.24 h for larvae and LT50= 64.08, 21.23 h for pupae;Cx. quinquefasciatus had LT50= 28.46, 20.25, 1.01 h for larvae and LT50= 67.52, 27.96,4.11 h for pupae, respectively. Oviposition deterrence of the oil was evaluated on gravid females. In the study, 10% Z. limonella showed 100% repellency for Ae. aegypti and99.53% for Cx. quinquefasciatus. Likewise, oviposition activity indexes of these oil concentrations were all negative values ranging from–0.89 to-1.00 for Ae. aegypti and–0.64 to–0.99 for Cx. quinquefasciatus. The oviposition activity indexes values revealed that Z. limonella oil has deterrent effect, and it caused a remarkable negative response resulting in very few ***: This result indicates that Z. limonella oil can be used as an effective adulticide, larvicide and oviposition deterrent against Ae. aegypti and Cx. quinquefasciatus.

关键词： Zanthoxylum limonella Aedes aepypti Culex quinquefasciatus Adulticide larvicide Oviposition deterrent

Larvicidal activity of endocarp and seed crude extracts of Dracaena loureiri Gagnep against Aedes aegypti(L.) mosquito

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 222-226页

作者： Damrongpan Thongwat Supaporn Lamlertthon Urat Pimolsri Nophawan Bunchu Department of Microbiology and Parasitology Faculty of Medical ScienceNaresuan UniversityPhitsanulokThailand Centre of Excellence in Medical Biotechnology Faculty of Medical ScienceNaresuan UniversityPhitsanulokThailand Centre of Excellence in Fungal Research Faculty of Medical ScienceNaresuan UniversityPhitsanulokThailand

Objective: To evaluate the larvicidal activity of the ethanolic and aqueous extracts of the endocarp and seeds of Dracaena loureiri(D. loureiri) against the dengue mosquito vector, Aedes ***: Bioassays were performed by exposing late third-stage to early fourth-stage larvae of Aedes aegypti to various concentrations of the extracts from D. loureiri. The larval mortality was observed after 24-and 48-h ***: The larvicidal bioassay in this study demonstrated that the ethanolic endocarp extract was the most effective with the LC50 value of 84.00 mg/L after 24 h exposure andlarvicide for controlling this mosquito species.

关键词： Aedes aegypti Dracaena loureiri larvicide Plant extracts Crude extracts

Evaluation of biological control agents for mosquitoes control in artificial breeding places

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 276-277页

作者： Salim Abadi Yaser Vatandoost Hassan Rassi Yavar Abai Mohammad Reza Sanei Dehkordi Ali Reza Paksa Azim Department of Medical Entomology and Vector Control School of Public HealthTehran University of Medical SciencesTehranIran

Objective:To evaluate the entomological impact of chlorpyrifos-methyl,Bacillus thuringiensis, and Gambusia affinis on mosquitoes control in artificial breeding ***:A Latin square design with 4 replicates was performed in order to evaluate the efficacy of chlorpyrifosmethyl, Bacillus thuringiensis,and Gambusia affinis on *** larvicide was applied at the dosage of 100 mg a.h/ha,Bacillus thuringiensis at the recommended dosage and 10 fishes per m^2 were applied at 1×1 m^2 artificial breeding *** larval densities for both anopheline and culicine were counted according to larvae /10 dippers prior and 24 h after ***: All three control agents are effective for mosquito density reduction,and the difference between the three agents and the control is significant(Plarvicide(P<0.05).Conclusions: Bacillus thuringiensis in comparison with two other agents is the appropriate method for larviciding in the breeding *** long term assessing for biological activities as well as monitoring and mapping of resistance is required.

关键词： larvicide Biological control Mosquito Iran

Mosquito larvicidal activity of Rauvolfia serpentina *** against Culex quinquefasciatus Say

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Asian Pacific Journal of Tropical Medicine 2012年第1期5卷 42-45页

作者： Dipanwita Das Goutam Chandra Mosquito and Microbiology Research Units Parasitology LaboratoryDepartment of ZoologyBurdwan UniversityWest Bengal713104India

Objective:To establish the larvicidal activities,if any of solvent extracts of Rauvolfia serpentina (***) *** against Culex quinquefasciatus(***) Say.1823 as target ***:Seeds of *** were extracted with five solvents graded according to the polarity[*** ***,ethyl acetate,acetone and absolute alcohol] continuing one after another with the same ***:Mortality rate with petroleum ether extract was significantly higher than other *** mortality rates of late 3rd instar larvae wore 50.33±5.***.10.00±1.***.0.00±0.***.21.33±1.53 and 0.00±0.00 in 100 ppm concentration of petroleum ether,benzene,ethyl acetate,acetone and absolute alcohol respectively,after 24 h of exposure ***:Results of this study show that petroleum ether extract of R. serpentina seed may be considered as a potent source of mosquito larvicidal agent.

关键词： Mosquito larvicide Rauvolfia serpentine Seeds Culex quinquefasciatus

Larvicidal activities of chinaberry,neem and Bacillus thuringiensis israelensis(Bti) to an insecticide resistant population of Anopheles arabiensis from Tolay,Southwest Ethiopia

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Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 554-561页

作者： Assalif Demissew Meshesha Balkew Melaku Girma Department of Medicine College of Medicine and Health SciencesAmbo University Aklilu Lemma Institute of Pathobiology Addis Ababa University Human Health Malaria Control DepartmentInternational Center of Insect Physiology and Ecology

Objective:To elucidate the larvicidal potency of neem, chinaberry and Bacillus thuringiensis israelensis(Bti) to larvae of Anopheles arabiensis under semi-field condition and adult susceptibility/resistance to the conventionally used insecticides in Tolay,Southwestern ***: Wild collected 3rd and 4th stage larvae were exposed to neem, and chinaberry seed powder dissolved in water and Bti in artificial containers at three treatment levels:0.2, 0.1 and 0.05 g/m^2 and controls were free of treatments. Larval and pupal mortalities were monitored daily and residual activities were determined. The experiments were replicated three times. The World Health Organization tube test for all classes of insecticides was conducted on adult Anopheles arabiensis reared from field collected larvae and pupae. Data were analyzed using STATA software version ***: In the first application, neem powder caused 88.9%, 87.9% and 79.4% larval and pupal mortality at 0.2, 0.1 and 0.05 g/m^2 after 4.3, 6.0 and 5.7 days, respectively. The corresponding killing effect of chinaberry was 80.3%, 62.1% and 30.3% after 7.0, 7.7 and 8.3days respectively. Bti at all treatments killed 100% after 24 h except 2.7 days for 0.05 g/m^*** mosquitoes were susceptible only for fenitrothion and pirimiphos-methyl with 100%mortality while resistant to deltamethrin, alpha-cypermethrin, etofenprox and dichlorodiphenyl-tricgloroethane with only 9.0%, 3.0%, 5.1% and 2.0% mortalities ***: Neem, chinaberry and Bti showed potent larvicidal and pupicidal activities. However, in the area, high level of mosquito resistance to pyrethroids and dichlorodiphenyl-tricgloroethane was seen which will pose serious challenge to vector control in the future. Therefore, using integrated approach including these botanical larvicides is warranted to manage insecticide resistance.

关键词： larvicide Anopheles arabiensis Tolay Insecticide Resistance Botanical Bio-efficacy