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Dietary sodium acetate and sodium butyrate improve high-carbohydrate diet utilization by regulating gut microbiota, liver lipid metabolism, oxidative stress, and inflammation in largemouth bass(Micropterus salmoides)

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1704-1722页

作者： Qiao Liu Liangshun Cheng Maozhu Wang Lianfeng Shen Chengxian Zhang Jin Mu Yifan Hu Yihui Yang Kuo He Haoxiao Yan Liulan Zhao Song Yang College of Animal Science and Technology Sichuan Agricultural UniversityChengdu 611130SichuanChina

Background Adequate level of carbohydrates in aquafeeds help to conserve protein and reduce cost. However, studies have indicated that high-carbohydrate(HC) diet disrupt the homeostasis of the gut–liver axis in largemouth bass, resulting in decreased intestinal acetate and butyrate *** Herein, we had concepted a set of feeding experiment to assess the effects of dietary sodium acetate(SA) and sodium butyrate(SB) on liver health and the intestinal microbiota in largemouth bass fed an HC diet. The experimental design comprised 5 isonitrogenous and isolipidic diets, including LC(9% starch), HC(18% starch), HCSA(18% starch;2 g/kg SA), HCSB(18% starch;2 g/kg SB), and HCSASB(18% starch;1 g/kg SA + 1 g/kg SB). Juvenile largemouth bass with an initial body weight of 7.00 ± 0.20 g were fed on these diets for 56 *** We found that dietary SA and SB reduced hepatic triglyceride accumulation by activating autophagy(ATG101, LC3B and TFEB), promoting lipolysis(CPT1α, HSL and AMPKα), and inhibiting adipogenesis(FAS, ACCA, SCD1 and PPARγ). In addition, SA and SB decreased oxidative stress in the liver(CAT, GPX1α and SOD1) by activating the Keap1-Nrf2 pathway. Meanwhile, SA and SB alleviated HC-induced inflammation by downregulating the expression of pro-inflammatory factors(IL-1β, COX2 and Hepcidin1) through the NF-κB pathway. Importantly, SA and SB increased the abundance of bacteria that produced acetic acid and butyrate(Clostridium_sensu_stricto_1). Combined with the KEGG analysis, the results showed that SA and SB enriched carbohydrate metabolism and amino acid metabolism pathways, thereby improving the utilization of carbohydrates. Pearson correlation analysis indicated that growth performance was closely related to hepatic lipid deposition, autophagy, antioxidant capacity, inflammation, and intestinal microbial *** In conclusion, dietary SA and SB can reduce hepatic lipid deposition;and alleviate oxidative stress and inflammation in largemout

关键词： High carbohydrate diet Intestinal microbiota largemouth bass Lipid deposition Sodium acetate Sodium butyrate

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Differential expression of miRNAs and mRNAs in the livers of largemouth bass Micropterus salmoides under heat stress

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Journal of Oceanology and Limnology 2024年第2期42卷 594-608页

作者： Xuqian ZHAO Zijie LIN Caijuan LI Hao ZHU Lingling LI Wenjia MAO Qufei LING School of Biology and Basic Medical Sciences Suzhou Medical College of Soochow UniversitySuzhou 215000China

Global warming threatens freshwater ecosystems and compromises fish *** elucidate the role of miRNAs in the livers of heat stressed largemouth bass,juvenile fish was subject to heat stress under 37°*** mRNA-seq and miRNA-seq were conducted on the liver tissues under control and heat stress *** gene expression analysis and enrichment analysis were performed on mRNA and miRNA expression profiles.A total of 406 differentially expressed genes(DEGs)were discovered,of which 212 were up-regulated and 194 were *** of the DEGs were significantly implicated in the regulation of“protein processing in endoplasmic reticulum”,“proteasome”,“steroid biosynthesis”,and“ornithine decarboxylase inhibitor activity”*** addition,47 differentially expressed miRNAs(DEMs)were identified in largemouth bass livers under heat stress,including 21 up-regulated and 25 down-regulated miRNAs.A negatively regulated miRNA-mRNA network including 12 miRNAs and 19 mRNAs was constructed with DEMs involved in“protein degradation”,“calcium ion regulation”,“cell apoptosis”,and“lipid metabolism”.Moreover,this study indicated novel-miR-144 activated the IRE1 signaling pathway by targeting txndc5 to induce liver apoptosis in largemouth bass under heat *** study revealed the involvement of miRNA regulation in largemouth bass in response to heat stress.

关键词： heat stress largemouth bass miRNA-mRNA interaction

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Whole-genome resequencing reveals recent signatures of selection in five populations of largemouth bass(Micropterus salmoides)

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Zoological Research 2023年第1期44卷 78-89页

作者： Cheng-Fei Sun Xin-Hui Zhang Jun-Jian Dong Xin-Xin You Yuan-Yuan Tian Feng-Ying Gao He-Tong Zhang Qiong Shi Xing Ye Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation Ministry of Agriculture and Rural AffairsPearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouGuangdong 510380China Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic AnimalsBGI Academy of Marine SciencesBGI MarineShenzhenGuangdong 518081China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

largemouth bass(Micropterus salmoides) is an economically important fish species in North America, Europe, and China. Various genetic improvement programs and domestication processes have modified its genome sequence through selective pressure, leaving nucleotide signals that can be detected at the genomic level. In this study,we sequenced 149 largemouth bass fish, including protospecies(imported from the US) and improved breeds(four domestic breeding populations from China). We detected genomic regions harboring certain genes associated with improved traits, which may be useful molecular markers for practical domestication, breeding, and selection. Subsequent analyses of genetic diversity and population structure revealed that the improved breeds have undergone more rigorous genetic changes. Through selective signal analysis, we identified hundreds of putative selective sweep regions in each largemouth bass line. Interestingly, we predicted 103 putative candidate genes potentially subjected to selection,including several associated with growth(psst1 and grb10), early development(klf9, sp4, and sp8), and immune traits(pkn2, sept2, bcl6, and ripk2). These candidate genes represent potential genomic landmarks that could be used to improve important traits of biological and commercial interest. In summary, this study provides a genome-wide map of genetic variations and selection footprints in largemouth bass, which may benefit genetic studies and accelerate genetic improvement of this economically important fish.

关键词： largemouth bass Whole-genome resequencing Signatures of selection Growth Immunity

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The direct and gut microbiota-mediated effects of dietary bile acids on the improvement of gut barriers in largemouth bass(Micropterus salmoides)

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Animal Nutrition 2023年第3期14卷 32-42页

作者： Rui Xia Qingshuang Zhang Dongmei Xia Qiang Hao Qianwen Ding Chao Ran Yalin Yang Aizhi Cao Zhen Zhang Zhigang Zhou China-Norway Joint Lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway-China Joint Lab on Fish Gut Microbiota Institute of BiologyNorwegian University of Science and TechnologyTrondheimNorway Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Shandong Longchang Animal Health Care Co. Ltd.Jinan 251100China Institute of Animal Husbandry and Veterinary Jiangxi Academy of Agricultural ScienceJiangxi 330000China

Fish gut barrier damage under intensive culture model is a significant concern for aquaculture *** study aimed to investigate the effects of bile acids(BAs)on gut barriers in Micropterus salmoides.A germ-free(GF)zebrafish model was employed to elucidate the effects of the direct stimulation of BAs and the indirect regulations mediated by the gut microbiota on gut barrier *** diets were formulated with BAs supplemented at 0,150,300 and 450 mg/kg,and these 4 diets were defined as control,BA150,BA300 and BA450,*** 5 weeks of feeding experiment,the survival rate of fish fed with BA300 diet was increased(P0.05).The results of the gut microbiota transferring experiment demonstrated an upregulation of gut barrier-related genes,including immunoglobulin Z/T(Ig Z/T),IL-6,IL-1βand IL-10,by the gut microbiota transferred from the BA300 group compared with the control(P<0.05).Feeding the BA300 diet directly to GF zebrafish resulted in enhanced expression of Ig M,Ig Z/T,lysozyme

关键词： Bile acids Gut barrier Gut microbiota largemouth bass Germ-free zebrafish

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Effects of dietary vitamin K_(3) levels on growth,coagulation,calcium content,and antioxidant capacity in largemouth bass,Micropterus salmoides

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Aquaculture and Fisheries 2023年第2期8卷 159-165页

作者： Xiang Wei Ying Hang Xiang Li Xueming Hua Xiangming Cong Wanting Yi Xu Guo Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityShanghai201306China Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of AgricultureShanghai Ocean UniversityShanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China

An 8-week feeding trial investigated the optimum dietary vitamin K3 requirements of largemouth bass.A total of 600 healthy fish(12.96±0.07 g)were fed diets containing varying levels of vitamin K3 at 0.78(K0),5.80(K5),10.82(K10),15.84(K15),and 20.85(K20)mg/kg with four replicates per *** showed that dietary vitamin K3 enhanced growth,with weight gain rate and specific growth rate in K15 trials significantly higher than K0 trials(P0.05).However,the supplementation of vitamin K3 significantly contributed to calcium accumulation in muscle tissues and vertebrae(P0.05).The antioxidant capacity was improved with the addition of vitamin K3,which was inconsistent with the changes in malondialdehyde and MK-4(a form of menaquinone)within liver and serum,suggesting that the toxicity of excessive artificial vitamin K3 may account for this *** regression analysis,the appropriate dietary vitamin K3 levels within a largemouth bass diet was determined to be in the range of 9.93-15.22 mg/kg,which will provide a reference for the preparation of vitamin premix and artificial diet for largemouth bass.

关键词： Growth Blood coagulation Antioxidant capacity largemouth bass Vitamin K3

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The potential of Clostridium autoethanogenum,a new single cell protein,in substituting fish meal in the diet of largemouth bass(Micropterus salmoides):Growth,feed utilization and intestinal histology

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Aquaculture and Fisheries 2023年第1期8卷 67-75页

作者： Pinxian Yang Xiaoqin Li Bowen Song Ming He Chaoyi Wu Xiangjun Leng National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityShanghai 201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 201306China

This study investigated the effects of replacing fishmeal with Clostridium autoethanogenum protein(CAP)on growth,feed utilization and intestinal histology of largemouth bass(Micropterus salmoides).The control diet contained 350 g/kg fishmeal,and then dietary fishmeal was decreased to 300 g/kg,250 g/kg,200 g/kg,150 g/kg and 100 g/kg by CAP inclusion,respectively(FM-30,FM-25,FM-20,FM-15,FM-10).The largemouth bass(110.0±1.0 g)were fed one of the six diets for 8 *** groups of FM-30,FM-25 and FM-20 showed the similar weight gain(WG)and feed conversion ratio(FCR)to the control(P>0.05),while the WG of FM-10 group was decreased by 15.9%,and FCR increased by 0.12 when compared to the control(P0.05).In conclusion,CAP could replace 150 g/kg fishmeal in diet(350 g/kg fishmeal)without adverse effects on growth,feed utilization and intestinal histology of largemouth bass.

关键词： largemouth bass Clostridium autoethanogenum protein Growth performance Feed utilization Intestinal histology

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The digestible phosphorus requirement in practical diet for largemouth bass(Micropterus salmoides)based on growth and feed utilization

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Aquaculture and Fisheries 2022年第6期7卷 632-638页

作者： Pu Wang Xiaoqin Li Zhen Xu Dong Ji Ming He JiangYu Dang Xiang-jun Leng National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityChina Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityChina Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityChina

The present study was conducted to estimate the optimum requirement of phosphorus in practical diets for largemouth bass(Micropterus salmoides).Six iso-energetic and iso-nitrogenous diets were formulated with the supplementation of monocalcium phosphate(MCP)of 0,5,10,15,20 and 25 g/kg in basal diet containing 350 g/kg fish meal,*** digestible phosphorus of the six diets was measured as 5.7,6.7,7.8,8.7,9.3 and 10.0 g/kg,*** diet was randomly fed to triplicate groups of 25 juvenile fish(initial body weight,16.0±0.2 g)for 60 *** results showed that weight gain increased,and feed conversion ratio decreased with the increase of dietary *** dietary MCP exceeded 15 g/kg(digestible phosphorus of 8.7 g/kg),the WG and FCR were maintained at the similar *** crude ash,phosphorus and calcium contents in whole body,apparent digestibility of phosphorus and protein retention also increased with the increasing phosphorus level in *** phosphorus and calcium levels and plasma phosphorus showed no further increase when dietary MCP reached 20,20 and 10 g/kg with digestible phosphorus of 9.3,9.3 and 7.8 g/kg,***-line analysis indicated that the digestible phosphorus requirement for largemouth bass were 8.9,8.7 and 9.6 g/kg based on weight gain,feed conversion ratio and vertebral phosphorus *** conclusion,the supplementation of MCP in a diet containing 350 g/kg fish meal improved the growth and feed utilization of largemouth bass,and the digestible phosphorus requirement was suggested to be 8.7–8.9 g/kg with MCP supplementation of 15.0–15.8 g/kg.

关键词： largemouth bass Digestible phosphorus Growth Feed utilization

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Effect of chicken haemoglobin powder on growth, feed utilization, immunity and haematological index of largemouth bass (Micropterus salmoides)

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Aquaculture and Fisheries 2020年第4期5卷 187-192页

作者： Guitao Ding Songlin Li An Wanga Naisong Chen National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Research Centre of the Agriculture Ministry on Environmental Ecology and Fish Nutrition Shanghai Ocean UniversityShanghai20136China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai201306China

A 12-week feeding trial was conducted to evaluate the effects of replacing fish meal by chicken haemoglobin powder in largemouth bass *** isonitrogenous(48%)and isolipidic(12%)diets were formulated to replace 0%(control),9.80%,19.61%and 29.41%of fish meal with chicken haemoglobin *** diet was fed to triplicate groups of fish(initial weight:49.50±0.07 g)twice *** fish specific growth rate was significantly reduced when diet replacement level was up to 19.61%,which may be related to the feed intake and apparent digestibility coefficient of protein and amino ***,the feed efficiency ratio and protein efficiency ratio were not significantly decreased until the replacement level up to 29.41%.The activity of CCP was significantly reduced when 19.61%of fish meal was ***,the activity of lysozyme and serum protein content was significantly altered in fish fed with the diets up to 29.41%***,the red blood cell count and haemoglobin content was significantly decreased when the replacement level was up to 19.61%.In overall,the diet with 9.80%of chicken haemoglobin powder was more suitable for largemouth bass.

关键词： Chicken haemoglobin meal Growth Feed utilization Non-specific immunity largemouth bass

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Evaluation of Stocking All Female largemouth bass (Micropterus salmoides) in Alabama (USA) Ponds

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Natural Resources 2016年第6期7卷 315-325页

作者： Michael J. Maceina Steven M. Sammons Ronald P. Phelps School of Fisheries Aquaculture and Aquatic Sciences Auburn University Auburn Alabama USA

Excessive largemouth bass ( Micropterus salmoides ) recruitment in small impoundments leads to density-dependent growth depression, causing populations to have length distributions skewed towards smaller fish. To address this issue, we conducted three trials that evaluated stocking all female largemouth bass (F-LMB) in two small Alabama ponds (0.5 and 2.0 ha) where the drainage basin was isolated. Age-1 fish were individually identified for sex using a micro pipette inserted in the urogenital pore, tagged, and stocked at 38-40 F-LMB/ha into ponds that contained pre-established sunfish ( Lepomis spp.) populations. Female largemouth bass were collected with electrofishing, angling, and rotenone over a 2.5- to 5-year period among the three trials. Sex was correctly identified for 179 of the 180 F-LMB stocked. One male was detected in the first trial which resulted in successful largemouth bass reproduction and this trial was terminated after two years. Growth was rapid the first 2 years after stocking as 3-year-old fish reached an average of 429 to 459 mm and 1.37 to 1.66 kg in all 3 trials. However, 2 years after stocking, growth was nil in the second trial even though relative weights of F-LMB were generally greater than 100. In this second trial, removal of about 30% of the F-LMB inhabiting the pond and stocking small Nile tilapia Oreochromis niloticus (25 - 75 mm) resulted in average weights increasing from 1.30 to 2.34 kg in 1 year. Growth of F-LMB continued in the third trail, and average size approached 500 mm and 2.2 kg three years after stocking. Annual survival rates of F-LMB were high and ranged from 0.78 to 0.93 among the 3 trials. Stocking F-LMB offers an attractive alternative in ponds to create a low density largemouth bass population that displays fast growth and high survival when catch-and-release fishing is primarily practiced.

关键词： largemouth bass Relative Weight Growth Survival Ponds Program MARK

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Assessment of dietary lysine requirement in largemouth bass,Micropterus salmoides

Assessment of dietary lysine requirement in largemouth bass,...

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The 14th International Symposium on Fish Nutrition & Feeding

作者： Naisong Chen~*,Hengyong Zhou,Jianzhong Ma,Jie Zhou Shanghai Ocean University,999 Huchen Guan Road,Shanghai 201306,PR China

An 89-day feeding trial was conducted to evaluate the quantitative requirement of dietary lysine in largemouth bass(initial weight 32±1g).Eight isonitrogenous and isoenergetic diets(diets 1-8) were formulated to contain crude protein 45.36%and gross energy 19.73 MJ/kg of dry diet. Diets 1-7 were formulated using plant protein and crystalline amino acids as a primary protein source to mold the EAA profile of largemouth bass body except for lysine under investigation. Seven levels of dietary lysine ranging from 1.88%to 4.18%of dry diet at an about 0.4% increment were achieved by adding zein-coated crystalline lysine at the expense of zein-coated crystalline *** 8 which was formulated using 48%white fishmeal as a major protein source without adding any crystalline amino acids was served as a positive control to examine whether feed intake and resulting growth of fish fed diets 1-7 would be affected by incorporating crystalline amino *** diet was randomly assigned to triplicate tanks connected to an indoor flow-through and aerated freshwater *** each tank,22 fish were fed by hand to apparent satiation twice daily(09:00 and 15:00).Water temperature was maintained at 25±1℃and light cycle 12:12 h.

关键词： largemouth bass Lysine requirement none-special immunity

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